Positive Direct Coombs Test Induced by Phenylhydrazine1

Muirhead, E. E.; Groves, M.; Bryan, Sandra

doi:10.1172/jci103050

Cited by 37 publications

(14 citation statements)

References 27 publications

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“…Heinz bodies were particularly obvious during the first three days of treatment with acetylphenylhydrazide, but almost disappeared by the end of the experimental period. Analogous results were obtained in a previous study involving smaller numbers of animals [Calvey, 1968]; similar findings have also been reported by other authors [Burt et al, 1951;Muirhead, Groves and Bryan, 1954;Finch, Hanson and Donohue, 1959 Fig. 4.…”

Section: Res'ultssupporting

confidence: 91%

INTRACELLULAR pH IN MATURE AND IMMATURE RABBIT RED CELLS

Calvey

1970

Exp Physiol

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The intracellular pH of rabbit red cells was determined from the distribution of (2-14C) DMO. Evidence is presented that the method provides a valid estimate of intracellular pH. In phosphate buffer at pH 7 40, the mean intracellular pH of the rabbit erythrocyte was 7-27. There was a close correspondence between the distribution ratios for hydrogen and chloride ions in normal rabbit blood. Values for intracellular pH, based on forty experiments in normal animals, were consistent with recent measurements of the membrane potential of mammalian red cells.The intracellular pH of the reticulocyte was deduced from the effect of acetylphenylhydrazide on the internal pH of rabbit red cells. At an external pH of 7 40, mean reticulocyte pH (7.32) was 0-06 pH units higher than mean erythrocyte pH (7.26).In preparations of reticulocytes, the distribution ratio for chloride ions was lower than the corresponding ratio for hydrogen ions. It is suggested that the presence in reticulocytes of small numbers of relatively alkaline mitochondria may be responsible for this phenomenon.Estimates Borsook, 1956], and may thus be relatively deficient in free non-penetrating protein anions and so able to equilibrate at a higher cell pH than the erythrocyte. The Gibbs-Donnan equilibrium across the red cell membrane might also be affected by the enhanced 238

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Section: Res'ultssupporting

confidence: 91%

INTRACELLULAR pH IN MATURE AND IMMATURE RABBIT RED CELLS

Calvey

1970

Exp Physiol

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“…The red blood cell count, hemoglobin concentration (17), hematocrit reading (18,19), reticulocyte count (20), white blood cell count, and -platelet count (direct and indirect methods) (20,21) were determined before the first nephrectomy, before the second nephrectomy, and in all but one group (explantation of renal cortex) 4 days after the second nephrectomy. On these occasions the direct antiglobulin (Coombs') test was also conducted on the circulating erythrocytes with the use of a canine antiglobulin serum prepared in -rabbits in our laboratory (22).…”

Section: Technique Of Analysismentioning

confidence: 99%

Renal Autoexplantation and Protection Against Renoprival Hypertensive Cardiovascular Disease and Hemolysis *

Muirhead¹,

Stirman²,

Jones³

1960

J. Clin. Invest.

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“…Muirhead, Groves and Bryan [6] were able to produce a positive Coombs tcst in dogs after phenylhydrazine administration which they considered to be due to some form of alteration of the erythrocytc by the drug or by its breakdown product but not to be dependant upon an immunological mechanism. Perna, Robinson and Steward [7] although using the same techniques were unable to confirm the results of Muirhead and coll.…”

Section: Discussionmentioning

confidence: 97%

“…Perna, Robinson and Steward [7] although using the same techniques were unable to confirm the results of Muirhead and coll. [6].…”

Section: Discussionmentioning

confidence: 99%