Positive effect of AgNPs and AuNPs in in vitro cultures of Lavandula angustifolia Mill.

Abstract

The aim of this study was determined how an addition of gold nanoparticle (AuNPs) and silver NPs (AgNPs) into culture media affects plant development and formation of oil glands in narrow-leaved lavender (Lavandula angustifolia) cv. 'Munstead'. Plant shoots were propagated on media supplemented with 1, 2, 5, 10, 20 and 50 mg dm −3 AuNPs or AgNPs (diameter of 24.2 ± 2.4 nm and 27.5 ± 4.8 nm). Both of NPs positively influenced the growth and development of lavender propagated in vitro. The culture media with NPs stimulated formation of shoots and increased plant weight. Roots of plants propagated on the media supplemented with NPs were usually longer than those in the control. Only high concentrations of NPs (20 and 50 mg dm −3) in the culture media were toxic to plants, as demonstrated by restricted shoot length and gradual decrease in the value of other morphological features. Increases in AgNPs concentration caused the number of secretory trichomes to decrease. The diameter of the trichomes on both sides of the leaf blade was larger when the plants were propagated on the media supplemented with 1 and 2 mg dm −3 NPs. The diameter of trichomes formed on the adaxial surface of the leaf blade was greatest in the media enriched with 2 mg dm −3 AgNPs and 5 mg dm −3 AuNPs, and smallest in the media enriched with 5 mg dm −3 AgNPs. The diameter of trichomes formed on the abaxial surface was largest in plants exposed to 1, 2, 5 and 10 mg dm −3 AuNPs, 1 mg dm −3 AgNPs, and smallest in plants exposed to 5 mg dm −3 AgNPs. Key message Silver and gold nanoparticles had a significantly improved the growth and development of narrow-leaved lavender propagated in vitro. The number and size of secretory trichomes formed on the leaves of narrow-leaved lavender grown in in vitro cultures depends on the concentration of silver or gold nanoparticles in the media. Keywords Micropropagation • Metal nanoparticles • Oil glands • Nanosilver • Nanogold Abbreviations NPs Nanoparticles AgNPs Silver nanoparticles AuNPs Gold nanoparticles MS Murashige and Skoog medium KIN Kinetin IAA 3-Indoleacetic acid Narrow-leaved lavender (Lavandula angustifolia Mill.) belonging to Lamiaceae family is an evergreen perennial plant with a high number of beneficial properties. The herb is native to the Mediterranean region, but is also grown in many other countries in the world (Wesołowska et al. 2019). Lavender infusions show carminative, diuretic, antirheumatic and anti-epileptic properties, and are effective painkillers, especially for nervous headaches and migraines (Gilani et al. 2000). Essential oils and extracts of the plant demonstrate antibacterial and antifungal properties (D'Auria et al. 2005). Apart from medicinal applications, lavender is Communicated by Nokwanda Pearl Makunga.

Impact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: Lavandula viridis L’Hér and Thymus lotocephalus G. López and R. Morales

“…Additionally, CuO and ZnO NPs also caused a significant increase in total antioxidant capacity, reducing power and DPPH radical scavenging capacity of the extracts from shoots of the same species [29,52]. There are no studies about the impact of the same type of NPs on the antioxidant activity of plants from the genus Lavandula and Thymus however, it was reported that Ag and Au NPs increased the ABTS free radical scavenging capacity in cultures of L. angustifolia [35].…”

“…Moreover, it has been shown that the application of nano-iron oxide increases the absorption and uptake of important macronutrients in several plant species [32][33][34]. On the other hand, the addition of other NPs such as Au and Ag at different concentrations positively affected in vitro growth and development of another Lavandula species, L. angustifolia [35].…”

Response of Phalaenopsis Orchid to Selenium and Bio-Nano-Selenium: In Vitro Rooting and Acclimatization

“…This previous method did not apply for all cases of orchid rooting, which needs a special handling. The use of nanomaterials in enhancing the plant rooting and its growth under in vitro conditions has become recently a promising tool such as silver nanoparticles (Thangavelu et al 2018;Saha and Gupta 2018;Jadczak et al 2019;Ha et al 2020;Tymoszuk•and Kulus 2020), iron nanoparticles (Mozafari et al 2018 and Khan et MAYADA K. SELIEM et al al. 2020), zinc oxide nanoparticles (Nalci et al 2019;Abdel Wahab et al 2020; Mazaheri-Tirani and Dayani 2020), cobalt nanoparticles (Ha et al 2020), gold nanoparticle (Jadczak et al 2019), copper oxide nanoparticles (Javed et al 2017 andNalci et al 2019), copper nanoparticles (Ibrahim et al 2019), nickel oxide nanoparticles (Pinto et al 2019), and nano-Se (Sotoodehnia-Korani et al 2020).…”

Nanotechnology in Plant Metabolite Improvement and in Animal Welfare

“…These molecules are of biological or non-biological origin and can recognize cytoplasmic membrane receptors on the plant cells. Recognition and binding led to signal elicitation, which stimulates the expression of genes related to secondary metabolite production [20][21][22]. The elicitors work as plant stressors in a natural environment: they can be represented by fungi, bacteria, activated enzymes, and other biological compounds [22,23].…”