Positive effect of ethanol-induced Lactococcus lactis on alcohol metabolism in mice

Chen, Sisi; Jia, Shimin; Suo, Keke; Kang, Qiaozhen; Hao, Limin; Lu, Laizheng; Liu, Xin; Huang, Jinyong; Lu, Jian

doi:10.1016/j.fshw.2022.04.016

Cited by 7 publications

(6 citation statements)

References 35 publications

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“…In humans, ROS act on lipids to cause peroxidation and the end product is malondialdehyde (MDA). Therefore, hepatic oxidative levels can be indicated by MDA levels [ 9 , 15 ]. As shown in Figure 2 g, the MDA level in the model group increased to 157.1% ( p < 0.05) of the normal group and decreased after 200 mg/kg·bw and 400 mg/kg·bw EPs treatments.…”

Section: Resultsmentioning

confidence: 99%

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Preparation, Structural Identification, and Screening of Egg-Derived Peptides with Facilitating Alcohol Metabolism Activity

Tan,

Wang,

Wan

et al. 2024

Foods

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The aim of this study was to obtain egg-derived peptides with facilitating alcohol metabolism (EPs) by enzymolysis, to identify their structures, and screen small polypeptides with higher activity by molecular docking. The optimum conditions for preparing EPs with facilitating alcohol metabolism were obtained by a single factor experiment, adding 2% Protamex and performing enzymolysis for 3 h with a liquid–material ratio of 35:1. The dose–response relationship experiment showed that 800 mg/kg·bw EPs played a better role in facilitating alcohol metabolism. EPs contained 40% hydrophobic amino acids (HAA), including 9.24% Leu. Eighty-four peptides were identified by HPLC-MS/MS and four peptides with potential activation of alcohol dehydrogenase were further selected by molecular docking. The tetrapeptide Trp-Ile-Val-Asp (WIVD) with the highest binding energy reached −7.16 kcal/mol. These findings suggest that egg is a good source for the preparation of peptides with facilitating alcohol metabolism activity.

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Section: Resultsmentioning

confidence: 99%

“…In recent years, many studies have been carried out on food-borne biomolecules with anti-alcohol effects [ 15 , 16 ]. Currently, these biomolecules can be roughly divided into the following four categories: polyphenols, polysaccharides, peptides, and microbial anti-alcohol metabolites [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation, Structural Identification, and Screening of Egg-Derived Peptides with Facilitating Alcohol Metabolism Activity

Tan,

Wang,

Wan

et al. 2024

Foods

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“…The mouse model of ALI, as previously described, was utilized. 15 After 7 days of acclimatization, all mice were randomly assigned 4 groups (n = 8), including the control group (Control), model group (Model), positive group (Bifendate), and FAPEG group (FAPEG). The control and model groups received saline treatment, the bifendate group was administered bifendate (150 mg/kg•bw), and the FAPEG group received FAPEG peptides solution (30 mg/kg•bw).…”

Section: Hepatoprotective Effects Of Fapeg Identified Through Virtual...mentioning

confidence: 99%

“…The mice had free access to sterile feed pellets (Henan Huanyu hekang Biotechnology Co., Ltd.) and distilled water. The mouse model of ALI, as previously described, was utilized …”

Section: Hepatoprotective Effects Of Fapeg Identified Through Virtual...mentioning

confidence: 99%

“…14 Our previous research observed that intracellular extracts of Lactococcus lactis (L. lactis) induced by ethanol increased ADH activity and improved alcohol metabolism in mice, suggesting that BAPs presented in stress-related proteins might play a relevant role. 15,16 In another study, proteins related to stress and metabolism, which were abundantly synthesized during yeast propagation, were identified as significant precursors of BAPs. 17 It was hypothesized that the ethanol stress-related proteins of L. lactis might serve as valuable precursors for ADH-activating peptides (Figure 1A).…”

Section: Introductionmentioning

confidence: 99%

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Identification of a Novel Peptide with Alcohol Dehydrogenase Activating Ability from Ethanol-Induced Lactococcus lactis: A Combined In Silico Prediction and In Vivo Validation

Chen,

Yi,

Kang

et al. 2024

J. Agric. Food Chem.

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Alcohol dehydrogenase (ADH) is a crucial rate-limiting enzyme in alcohol metabolism. Our previous research found that ethanol-induced intracellular extracts of Lactococcus lactis (L. lactis) could enhance alcohol metabolism in mice, but the responsible compounds remain unidentified. The study aimed to screen potential ADH-activating peptides from ethanol-induced L. lactis using virtual screening and molecular docking calculation. Among them, the pentapeptide FAPEG might bind to ADH through hydrophobic interaction and hydrogen bonds, then enhancing ADH activity. Spectroscopy analysis further investigated the peptide-enzyme interaction between FAPEG and ADH, including changes in the amino acid residue microenvironment and secondary structural alterations. Furthermore, FAPEG could protect against alcoholic liver injury (ALI) in mice by reducing blood alcohol concentration, enhancing the activity of antioxidant and alcohol metabolism enzymes, and attenuating alcohol-induced hepatotoxicity, which was related to the activation of the Nrf2/keap1/HO-1 signaling pathway. The study provided preliminary evidence that the generation of ADH-activating peptides in ethanol-induced L. lactis has the potential in preventing ALI in mice using in silico prediction and in vivo validation approaches.

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Probiotic properties and proteomics analysis of ethanol-induced Lactococcus lactis subsp. lactis IL1403

Chen,

Yi,

Suo

et al. 2023

World J Microbiol Biotechnol

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Positive effect of ethanol-induced Lactococcus lactis on alcohol metabolism in mice

Cited by 7 publications

References 35 publications

Preparation, Structural Identification, and Screening of Egg-Derived Peptides with Facilitating Alcohol Metabolism Activity

Preparation, Structural Identification, and Screening of Egg-Derived Peptides with Facilitating Alcohol Metabolism Activity

Identification of a Novel Peptide with Alcohol Dehydrogenase Activating Ability from Ethanol-Induced Lactococcus lactis: A Combined In Silico Prediction and In Vivo Validation

Probiotic properties and proteomics analysis of ethanol-induced Lactococcus lactis subsp. lactis IL1403

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