Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur

Toğral, Güray; Arıkan, Murat; Korkusuz, Petek; Hesar, Ramin Hashemi; Ekşioğlu, Mehmet Fatih

doi:10.5606/ehc.2015.29

Cited by 23 publications

(27 citation statements)

References 32 publications

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“…Several experimental studies testing the positive or negative effects of different drugs or chemicals on bone healing have been published in the literature. [1][2][3] Pregabalin is a widely used drug for the treatment of diabetic peripheral neuropathy, postherpetic neuralgia, spinal cord-originated nerve pain, fibromyalgia, and neuropathic arthropathies. As an adjunct drug for reducing postoperative pain, it may also be administered in surgically treated patients.…”

mentioning

confidence: 99%

Pregabalin does not affect fracture healing adversely

Koçkara

Sofu

Issın

et al. 2017

Eklem Hastalik Cerrahisi

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mentioning

confidence: 99%

Pregabalin does not affect fracture healing adversely

Koçkara

Sofu

Issın

et al. 2017

Eklem Hastalik Cerrahisi

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“…This implication is particularly important in a clinical sense because an increasing number of studies are assessing the influence of medication (e.g. rivaroxaban and tadalafil) on fracture healing and bone remodelling, respectively [25, 26]. …”

Section: Discussionmentioning

confidence: 99%

Fully automated segmentation of callus by micro-CT compared to biomechanics

et al. 2017

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BackgroundA high percentage of closed femur fractures have slight comminution. Using micro-CT (μCT), multiple fragment segmentation is much more difficult than segmentation of unfractured or osteotomied bone. Manual or semi-automated segmentation has been performed to date. However, such segmentation is extremely laborious, time-consuming and error-prone. Our aim was to therefore apply a fully automated segmentation algorithm to determine μCT parameters and examine their association with biomechanics.MethodsThe femura of 64 rats taken after randomised inhibitory or neutral medication, in terms of the effect on fracture healing, and controls were closed fractured after a Kirschner wire was inserted. After 21 days, μCT and biomechanical parameters were determined by a fully automated method and correlated (Pearson’s correlation).ResultsThe fully automated segmentation algorithm automatically detected bone and simultaneously separated cortical bone from callus without requiring ROI selection for each single bony structure. We found an association of structural callus parameters obtained by μCT to the biomechanical properties. However, results were only explicable by additionally considering the callus location.ConclusionsA large number of slightly comminuted fractures in combination with therapies that influence the callus qualitatively and/or quantitatively considerably affects the association between μCT and biomechanics. In the future, contrast-enhanced μCT imaging of the callus cartilage might provide more information to improve the non-destructive and non-invasive prediction of callus mechanical properties. As studies evaluating such important drugs increase, fully automated segmentation appears to be clinically important.

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“…The specimens were then embedded in paraffin and cut longitudinally. Sagittal sections (5 µm) were stained with H&E for assessment of basic morphology and with Masson's trichrome for analysis of bone and cartilage using a standard histological staining protocol (25,26). …”

Section: Methodsmentioning

confidence: 99%

Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice

Huang

et al. 2018

Exp Ther Med

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The advantages of combining local delivery of bone morphogenetic protein (BMP)-2 with systemic or local anti-osteoporosis treatments have also been studied for enhancing osteoporotic fracture healing. The aim of the present study was to evaluate the effect of combination therapy with BMP-2 and psoralen on fracture repair in ovariectomized mice. At 6 weeks after bilateral ovariectomy, mice (n=30) underwent unilateral transverse osteotomy on the femur and were divided into 3 groups. In the model group (n=10), animals were implanted with an absorbable collagen sponge (ACS) alone and administered physiological saline intragastrically (i.g.). In the recombinant human (rh)BMP-2 group (n=10), animals were implanted with an ACS loaded with 2.5 µg rhBMP-2 and administered physiological saline i.g. In the psoralen + rhBMP-2 group (n=10) animals were implanted with an ACS loaded with 2.5 µg rhBMP-2 and administered psoralen i.g. The mice were euthanized after 21 days and their fractured femurs were assessed by micro computed tomography, histological analysis and biomechanical testing. Furthermore, the serum of the animals was analyzed. Psoralen + rhBMP-2 exerted more beneficial effects on callus consolidation and biomechanical strength. In addition, increased bone-specific alkaline phosphatase levels and decreased C-terminal telopeptide of type-1 collagen were observed in the Psoralen + rhBMP-2 group. However, no difference in estrogen levels was detected between the groups. In conclusion, the present study demonstrated that in ovariectomized mice, combination of locally delivered BMP-2 and systemically administered psoralen improved bone healing compared with BMP-2 alone.

show abstract

Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur

Cited by 23 publications

References 32 publications

Pregabalin does not affect fracture healing adversely

Pregabalin does not affect fracture healing adversely

Fully automated segmentation of callus by micro-CT compared to biomechanics

Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice

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