Positive effects of ceftriaxone on pentylenetetrazol-induced convulsion model in rats

Uyanıkgil, Yiğit; Özkeşkek, Kübra; Çavuşoğlu, Türker; Solmaz, Volkan; Tümer, Mehmet Kemal; Erbaş, Oytun

doi:10.3109/00207454.2014.991821

Cited by 26 publications

(25 citation statements)

References 23 publications

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“…In acute conditions, PTZ-induced elevated MDA levels in brain have been reported in various studies such as Uyanikgil et al 26 and Cevik et al 45 . In these studies, 70 mg/kg of PTZ was used to induce convulsions in rats, as in our study, and findings of the present study are in good accordance with the results of the above-mentioned studies.…”

Section: Discussionmentioning

confidence: 86%

“…The electrodes (polyamide-coated stainless steel wires, 0.1 mm diameter and electrical resistance <1Ω/10 mm) were implanted on dura over left frontal cortex (2.0 mm lateral to the midline, 1.5 mm anterior to the bregma 24 ) and the reference electrode was implanted over cerebellum (1.5 mm posterior to the lambda, on midline 24 ). The electrodes were fixed by using dental acrylic following the implantation 25,26 .…”

Section: Methodsmentioning

confidence: 99%

“…All groups, except Group-A1, were received 35 mg/kg PTZ and EEG was recorded. EEG recordings were taken in awake rats in a special container after 5 minutes from PTZ administration 26 . The EEG recordings were taken in 60 minutes 26 , the signals were amplified 10.000 times and also filtered with a range of 1-60 Hz.…”

Section: Methodsmentioning

confidence: 99%

“…EEG recordings were taken in awake rats in a special container after 5 minutes from PTZ administration 26 . The EEG recordings were taken in 60 minutes 26 , the signals were amplified 10.000 times and also filtered with a range of 1-60 Hz. EEG records were taken by using the BIOPAC MP150 Data Acquisition System (Biopac System Inc., Santa Barbara, USA) and spike percentage (We defined "spike percentage" as a reproducible way of quantifying epileptiform activity is to quantify the percentage of 1-second bins with at least one spike-wave 27 ) was evaluated.…”

Section: Methodsmentioning

confidence: 99%

“…All administrations were done ip. Racine's Convulsion Scale (RCS) and onset times of "first myoclonic jerk" (FMJ) was used to evaluate the seizures (for only 70 mg/kg PTZ) as follows: 0 = no convulsion; 1 = twitching of vibrassae and pinnae; 2 = motor arrest with more pronounced twitching; 3 = motor arrest with generalized myclonic jerks (this time was recorded for evaluating FMJ onset time); 4 = tonic-clonic seizure while the animal remained on its feed; 5 = tonic-clonic seizure with loss of the righting reflex; 6 = lethal seizure 25,26 . The onset times were recorded as seconds.…”

Section: Methodsmentioning

confidence: 99%

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Acute Administration of High Dose Trimetazidine Inhibits Pentylenetetrazol-Induced Seizures in Rats

Gürgül¹,

Erten

Gül³

et al. 2017

Cumhuriyet Medical Journal

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SUMMARYObjective: Resistant epilepsy is widely seen in clinical settings manipulate the researchers to pursuit novel anti-epileptic treatments. Although trimetazidine (TMZ) is commonly used in the treatment of angina and ischemic diseases, it is considered that the drug may be beneficial in the treatment of epileptic seizures and convulsions. We aimed to evaluate electrophysiologically the protective effects of high dose acute TMZ on epileptic seizures and convulsions in a rat model of epilepsy induced by pentilentetrazol (PTZ). Method: Forty-eight male Sprague-Dawley rats were used for the EEG evaluation (Group-A; n=24) and behavioral assessment (Group-B; n=24). Grup-A1 and Grup-B1 were determined as control group and given no medication. 35 mg/kg (Group-A2, -A3 and -A4) and 70 mg/kg (Group-B2, -B3 and -B4) of PTZ were administered intraperitoneally (ip.) so as to generate seizures and convulsions. Group-A2 and Group-B2 were treated with saline. 40 mg/kg (Grup-A3 and Grup-B3) and 80 mg/kg of TMZ (Group-A4 and Group-B4) were administered to the treatment groups by ip. Brain malondialdehyde (MDA) levels, spike-percentage values, Racine's Convulsion Scale (RCS) scores and "first myoclonic jerk (FMJ)" latencies of each rat were determined. Results: Brain MDA levels, spike-percentage values, RCS scores, and FMJ latencies of Group-A2 and Group-B2 were significantly increased compared to those of the control's (Grup-A1 and Grup-B1) (P<0.05 for all comparisons). However, in TMZ treated groups (Group-A3 and -A4; Group-B3 and -B4) the elevated values of these parameters were found to be significantly decreased (P<0.05 for all comparisons). Conclusions:This study has demonstrated that the high dose acute TMZ administration may prevent PTZ-induced seizures and convulsions in rats. Furthermore, it can be also said that TMZ has a neuroprotective effects on oxidative stress associated with PTZ. Therefore, it may be considered for use with therapeutic purposes of TMZ in resistant epilepsy patients in the future. Keywords: Electroencephalography (EEG), Epilepsy, Malondialdehyde, Pentylenetetrazol, Rat, Trimetazidine.ÖZET Amaç: İlaca-dirençli epilepsi, klinikte yaygın olarak görülen ve araştırmacıları yeni anti-epileptik tedavilerin saptanması için harekete geçiren bir durumdur. Trimetazidin (TMZ) anjina ve iskemik hastalıkların tedavisinde yaygın olarak kullanılmasına rağmen, epileptik nöbetler ve konvülsiyonların tedavisinde de yararlı olabileceği düşünülmektedir. Bu çalışmada, pentilentetrazol (PTZ) ile indüklenen bir sıçan epilepsi modelinde yüksek doz akut TMZ'nin epileptik nöbetler ve konvülsiyonlar üzerine koruyucu etkilerini elektrofizyolojik parametreler açısından değerlendirmeyi amaçladık. 381Yöntem: 48 adet erkek Sprague-Dawley cinsi sıçan EEG (Group-A; n=24) ve davranış analizlerinde (Group-B; n=24) kullanıldı. Grup-A1 ve Grup-B1 kontrol grubu olarak belirlendi ve ilaç verilmedi. 35 mg/kg (Group-A2, -A3 ve -A4) ve 70 mg/kg PTZ (Group-B2, -B3 ve -B4), nöbet ve konvülsiyon oluşturmak amacıyla, intraperitoneal olarak (i...

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Section: Discussionmentioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Acute Administration of High Dose Trimetazidine Inhibits Pentylenetetrazol-Induced Seizures in Rats

Gürgül¹,

Erten

Gül³

et al. 2017

Cumhuriyet Medical Journal

Self Cite

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Antiallodynic Activity of Ceftriaxone and Clavulanic Acid in Acute Administration is Associated with Serum TNF‐α Modulation and Activation of Dopaminergic and Opioidergic Systems

Ochoa-Aguilar

Sotomayor-Sobrino

Jaimez

et al. 2017

Drug Development Research

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Preclinical Research The aim of this study was to determine the antiallodynic effect of acute administration of the β-lactam antimicrobials, ceftriaxone (CFX) and clavulanic acid (CLAV), for the control of established pain on a model of neuropathic pain (NP). We also investigated the involvement of dopaminergic and opioidergic pathways as well as alterations in serum concentrations of TNF-α in the antiallodynic actions of these drugs. CFX, CLAV, or gabapentin (GAP), a reference drug, were administered i.p. twelve days after constriction of the sciatic nerve in rats. Mechanic and cold allodynia were evaluated for 3 h and alterations in serum concentration of TNF-α determined. Both CFX and CLAV had antiallodynic effects in response to mechanical and cold stimulation, similar to GAP. The antiallodynic effects of CFX and CLAV were blocked by haloperidol (HAL), a D2 receptor antagonist, and by naloxone (NLX), an opioid receptor antagonist. Additionally, serum TNF-α levels were attenuated following CFX and CLAV administration. These results suggest that acute administration of CFX and CLAV may represent a promising approach for treating the acute allodynia of NP, and that the mechanisms involved in these effects involve activation of dopaminergic and opioidergic pathways as well as modulation of TNF-α production. Drug Dev Res 78 : 105-115, 2017. © 2017 Wiley Periodicals, Inc.

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Ceftriaxone pretreatment confers neuroprotection in rats with acute glaucoma and reduces the score of seizures induced by pentylenotetrazole in mice

Fachim

Guizzo

Cunha

et al. 2020

J Biochem & Molecular Tox

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β‐Lactam antibiotics such as ceftriaxone, are potent stimulators of the expression of l‐glutamate transporter GLT‐1 and may exert neuroprotective effects when chronically used in rats and mice. In this study, we used two animal models to test the neurological effect of subchronic treatment with ceftriaxone: experimental acute glaucoma in Wistar rats and induction of acute seizures with pentylenetetrazole in mice. We also assessed the performance of mice in the rotarod to calculate therapeutic indexes and exploratory activity in the open field. Our results showed that subchronic use of ceftriaxone was neuroprotective in both models, reducing injury in acute ischemia and ischemia/reperfusion in specific layers of retina and leading to a decrease in the seizure severity score. In behavioral experiments, we observed that ceftriaxone increased hyperactivity followed by a decrease in exploratory behavior in the open field, and there was no motor impairment in the rotarod test. We conclude that ceftriaxone may be useful as a tool in the development of new neuroprotective drugs targeting diseases which present a possible dysfunction in the balance of glutamatergic neurotransmission.

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Positive effects of ceftriaxone on pentylenetetrazol-induced convulsion model in rats

Abstract: Our study demonstrated that ceftriaxone has protective effects on PTZ-induced convulsions and on oxidative damage associated with PTZ.

Cited by 26 publications

References 23 publications

Acute Administration of High Dose Trimetazidine Inhibits Pentylenetetrazol-Induced Seizures in Rats

Acute Administration of High Dose Trimetazidine Inhibits Pentylenetetrazol-Induced Seizures in Rats

Antiallodynic Activity of Ceftriaxone and Clavulanic Acid in Acute Administration is Associated with Serum TNF‐α Modulation and Activation of Dopaminergic and Opioidergic Systems

Ceftriaxone pretreatment confers neuroprotection in rats with acute glaucoma and reduces the score of seizures induced by pentylenotetrazole in mice

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