Positive Feedback Stimulation of Ccnb1 and Mos mRNA Translation by MAPK Cascade During Mouse Oocyte Maturation

Cao, Lan-Rui; Jiang, Jun‐Chao; Fan, Heng‐Yu

doi:10.3389/fcell.2020.609430

Cited by 28 publications

(23 citation statements)

References 37 publications

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“…As oocyte nuclear maturation progression determines PBE, it was postulated that CPF could inhibit oocyte nuclear maturation progression, and this hypothesis was supported by the results that oocyte nuclear maturation progression was inhibited in the CPF group, with the obviously downregulated percentages of oocytes at the time points of MI, AI/TI and MII events and arrested proportions of oocytes at GVBD and MI when AI/TI and MII events occurred. The reason may be due to the interference of CPF on the expression of meiotic cell cycle factors, as meiotic progression is mainly regulated by MPF and MAPK (Cao et al, 2020). The obviously downregulated expression of Cdk1, Ccnb1, and MAPK3 confirmed this point.…”

Section: Discussionmentioning

confidence: 71%

Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation

Guo¹,

Zhao²,

Ye³

2021

Front. Cell Dev. Biol.

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Chlorpyrifos (CPF), a widely used organophosphate pesticide, is reported to severely impair mammalian reproductive system. Pterostilbene (PTS), an effective free radical scavenger, is considered as beneficial for mammalian reproduction. However, the toxicity of CPF on oocyte maturation and whether PTS can eliminate the detrimental effect of CPF on oocytes remain unclear. Here, porcine oocytes were applied to investigate the potential effect and possible mechanism of CPF and PTS during oocyte maturation. This work demonstrated that CPF significantly delayed the meiotic progression and decreased the polar body extrusion by disturbing spindle assembly and chromosome alignment and causing DNA damage in oocytes (p < 0.05). And, CPF significantly impaired oocyte cytoplasmic maturation by inducing the high level of reactive oxygen species and decreasing glutathione content (p < 0.05). Moreover, CPF significantly triggered embryo apoptosis and reduced the blastocyst rate and cell number following parthenogenetic activation (p < 0.05). Whereas CPF-exposed oocytes were treated with PTS, these defects caused by CPF were obviously rescued, and oocyte maturation and subsequent embryonic development were also significantly ameliorated (p < 0.05). In conclusion, these results revealed that CPF exerted the toxic effect on porcine oocytes, while PTS effectively alleviated CPF-induced damage on oocytes. This work provides a potential strategy to protect oocyte maturation in mammalian species.

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Section: Discussionmentioning

confidence: 71%

Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation

Guo¹,

Zhao²,

Ye³

2021

Front. Cell Dev. Biol.

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“…Despite MOS protein’s translation after GVBD, previous studies have revealed that precocious MOS translation in GV oocytes facilitates meiotic maturation (Cao et al , 2020 ). To test whether these MOS variants altered this function, we microinjected mCherry and MOS variants mRNAs (500 ng/ul for the homozygous variants, and each 250 ng/µl with mixed total 500 ng/µl for the compound heterozygous variants) in GV oocytes and maintained in milrinone‐treated medium for 24 h, and then counted the numbers of GV, GVBD, and MII oocytes (Fig EV1A ).…”

Section: Resultsmentioning

confidence: 99%

“…The MOS mRNA is highly expressed in oocyte, and MOS protein is actively translated during oocyte maturation and rapidly degraded after fertilization, in several vertebrates, including human (Sagata et al , 1988 ; Watanabe et al , 1991 ; Sha et al , 2020b ). Despite that MOS protein is nearly untranslated in oocyte at GV stage, precocious activation MOS‐ERK signal cascade by microinjection of Mos mRNAs in mouse immature oocytes could promote cyclin B1 translation and maturation promoting factor (MPF) activation, leading to oocyte meiotic maturation resumption (Choi et al , 1996b ; Cao et al , 2020 ). Deficiency of Mos or Erk1/2 in mice results in oocyte MII arrest failure, spindle abnormality, large polar body, and early embryo developmental arrest (Colledge et al , 1994 ; Hashimoto et al , 1994 ; Araki et al , 1996 ; Choi et al , 1996a ; Zhang et al , 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Biallelic mutations in MOS cause female infertility characterized by human early embryonic arrest and fragmentation

et al. 2021

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Early embryonic arrest and fragmentation (EEAF) is a common phenomenon leading to female infertility, but the genetic determinants remain largely unknown. The Moloney sarcoma oncogene (MOS) encodes a serine/threonine kinase that activates the ERK signaling cascade during oocyte maturation in vertebrates. Here, we identified four rare variants of MOS in three infertile female individuals with EEAF that followed a recessive inheritance pattern. These MOS variants encoded proteins that resulted in decreased phosphorylated ERK1/2 level in cells and oocytes, and displayed attenuated rescuing effects on cortical F‐actin assembly. Using oocyte‐specific Erk1/2 knockout mice, we verified that MOS‐ERK signal pathway inactivation in oocytes caused EEAF as human. The RNA sequencing data revealed that maternal mRNA clearance was disrupted in human mature oocytes either with MOS homozygous variant or with U0126 treatment, especially genes relative to mitochondrial function. Mitochondrial dysfunction was observed in oocytes with ERK1/2 deficiency or inactivation. In conclusion, this study not only uncovers biallelic MOS variants causes EEAF but also demonstrates that MOS‐ERK signaling pathway drives human oocyte cytoplasmic maturation to prevent EEAF.

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“…These proteins have been well elucidated as maternal proteins necessary for oogenesis. For instance, cyclin B1 (CCNB1) and proto-oncogene serine/threonine-protein kinase mos (MOS) are required for maturation promoting factor activation and mitogen-activated protein kinase cascade (58). The spindle checkpoint signaling depends on (BUB1) and serine/threonine-protein kinase PLK (PLK1) (59).…”

Section: Discussionmentioning

confidence: 99%

Proteomic Exploration of Porcine Oocytes During Meiotic Maturation in vitro Using an Accurate TMT-Based Quantitative Approach

et al. 2022

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The dynamic changes in protein expression are well known to be required for oocyte meiotic maturation. Although proteomic analysis has been performed in porcine oocytes during in vitro maturation, there is still no full data because of the technical limitations at that time. Here, a novel tandem mass tag (TMT)-based quantitative approach was used to compare the proteomic profiles of porcine immature and in vitro mature oocytes. The results of our study showed that there were 763 proteins considered with significant difference−450 over-expressed and 313 under-expressed proteins. The GO and KEGG analyses revealed multiple regulatory mechanisms of oocyte nuclear and cytoplasmic maturation such as spindle and chromosome configurations, cytoskeletal reconstruction, epigenetic modifications, energy metabolism, signal transduction and others. In addition, 12 proteins identified with high-confidence peptide and related to oocyte maturation were quantified by a parallel reaction monitoring technique to validate the reliability of TMT results. In conclusion, we provided a detailed proteomics dataset to enrich the understanding of molecular characteristics underlying porcine oocyte maturation in vitro.

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Positive Feedback Stimulation of Ccnb1 and Mos mRNA Translation by MAPK Cascade During Mouse Oocyte Maturation

Cited by 28 publications

References 37 publications

Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation

Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation

Biallelic mutations in MOS cause female infertility characterized by human early embryonic arrest and fragmentation

Proteomic Exploration of Porcine Oocytes During Meiotic Maturation in vitro Using an Accurate TMT-Based Quantitative Approach

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