Positive interaction between amino and sulfhydryl groups for acrylamide removal

Elimination of Acrylamide by Moderate Heat Treatment below 120°C with Lysine and Cysteine

“…The E a of lysine and cysteine reactions with aqueous acrylamide were 34.1 kJ/mol and 45.3 kJ/mol, respectively. Hidalgo et al (2011) reported that the E a for the reaction between acrylamide and cysteine was 24.4 kJ/mol. Their reaction was, however, carried out at pH 7.0 using a phosphate buffer and at a water activity of 0.95.…”

“…Claeys et al (2005) kinetically analyzed the effect of amino acids on acrylamide formation/ elimination by heating a mixture of asparagine and glucose at temperatures between 140 and 200℃, and reported that the presence of cysteine or lysine significantly reduced acrylamide formation, whereas glutamine promoted the formation of acrylamide. There are also several studies reporting the reaction of acrylamide with amino acids such as glycine, lysine and cysteine at high temperatures (Adams et al, 2010;Liu et al, 2011;Zamora et al, 2010;Hidalgo et al, 2011). The addition of amino acids other than asparagine reduced acrylamide formation in homogenized potatoes heated at 180℃ for 20 min, probably due to the competitive consumption of acrylamide precursors and/or elimination of produced acrylamide by nucleophilic components in the amino acids (Rydberg et al, 2003).…”

Use of Nucleophilic Compounds, and Their Combination, for Acrylamide Removal

“…In this sense, Hidalgo et al [38] studied the reaction of acrylamide with glutamic acid (as an amino acid having only an amino group), N-acetylcysteine (as an amino acid having only a thiol group), the mixture of glutamic acid and N-acetylcysteine (where both amino and thiol groups are present but in different molecules), glutathione (where both amino and thiol groups are present in the same molecule but not close together), and cysteine (where amino and thiol groups are closer than in glutathione). However, there are very few studies that try to determine if this combination of nucleophilic groups is positive for acrylamide removal.…”

Relationship between Antioxidants and Acrylamide Formation

“…For further understanding, Hidalgo et al [41] tested the removing effect of amino and sulfhydryl groups toward acrylamide using glutamic acid (Glu, with an amino group),…”