“Positive Regulation of RNA Metabolic Process” Ontology Group Highly Regulated in Porcine Oocytes Matured<i> In Vitro</i>: A Microarray Approach

Celichowski, Piotr; Nawrocki, Mariusz J.; Dyszkiewicz-Konwińska, Marta; Jankowski, Maurycy; Budna, Joanna; Bryja, Artur; Kranc, Wiesława; Borys, Sylwia; Knap, Sandra; Ciesiółka, Sylwia; Ješeta, Michal; Piasecka-Stryczyńska, Karolina; Khozmi, Ronza; Bukowska, Dorota; Antosik, Paweł; Brüssow, Klaus‐Peter; Bruska, M; Nowicki, Michał; Zabel, Maciej; Kempisty, Bartosz

doi:10.1155/2018/2863068

Cited by 13 publications

(14 citation statements)

References 41 publications

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“…The main mechanism for the proper maturation and growth of mammalian oocytes is the organization by adequate storage of a large amount of mRNA and proteins. As shown in previous studies of our team [6], both nuclear (reaching MII) and cytoplasmic maturation of oocytes are a key step before successful fertilization, zygote formation, and then the growth and development of early embryos.…”

Section: Discussionsupporting

confidence: 58%

Protein oligomerization is the biochemical process highly up-regulated in porcine oocytes before in vitro maturation (IVM)

Borys-Wójcik

Kocherova

Celichowski

et al. 2018

Medical Journal of Cell Biology

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A wide variety of mechanisms controlling oligomerization are observed. The dynamic nature of protein oligomerization is important for bioactivity control. The oocyte must undergo a series of changes to become a mature form before it can fully participate in the processes associated with its function as a female gamete. The growth of oocytes in the follicular environment is accompanied by surrounding somatic cumulus (CCs) and granulosa cells (GCs). It has been shown that oocytes tested before and after in vitro maturation (IVM) differ significantly in the transcriptomic and proteomic profiles. The aim of this study was to determine new proteomic markers for the oligomerization of porcine oocyte proteins that are associated with cell maturation competence. The Affymetrix microarray assay was performed to examine the gene expression profile associated with protein oligomerization in oocytes before and after IVM. In total, 12258 different transcriptomes were analyzed, of which 419 genes with lower expression in oocytes after IVM. We found 9 genes: GJA1, VCP, JUP, MIF, MAP3K1, INSR, ANGPTL4, EIF2AK3, DECR1, which were significantly down-regulated in oocytes after IVM (in vitro group) compared to oocytes analyzed before IVM (in vivo group). The higher expression of genes involved in the oligomerization of the protein before IVM indicates that they can be recognized as important markers of biological activation of proteins necessary for the further growth and development of pig embryos.

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Section: Discussionsupporting

confidence: 58%

Protein oligomerization is the biochemical process highly up-regulated in porcine oocytes before in vitro maturation (IVM)

Borys-Wójcik

Kocherova

Celichowski

et al. 2018

Medical Journal of Cell Biology

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“…It promotes angiogenesis in the ovulatory follicle [20]. The studies on bovine oocytes after in vitro maturation showed that supplementation with VEGF had a positive impact on cytoplasm maturation and oocyte developmental capacity [21]. We discovered that VEGFA interacts with other genes including INSR and KIT, which also belong to both ontology groups (Fig.…”

Section: Discussionmentioning

confidence: 83%

Analysis of expression of genes responsible for regulation of cellular proliferation and migration – microarray approach based on porcine oocyte model

Chamier-Gliszczyńska

Kałużna

Stefańska

et al. 2019

Medical Journal of Cell Biology

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The formation of mammalian oocytes begins in the ovary during fetal development. The proper development of oocytes requires close communication with surrounding somatic cells, the substances they emit allow proper maturation of oocytes. Somatic cumulus (CC) cells and oocytes form cumulus-oocyte (COC) complexes.In this study, the Affymetrix microarray analysis was used to investigate changes in gene expression occurring in oocytes before and after in vitro maturation (IVM). The aim of the study was to examine oocyte genes involved in two ontological groups, “regulation of cell migration” and “regulation of cell proliferation” discovered by the microarray method.We found a reduced expression of all 28 genes tested in the ontological groups: ID2, VEGFA, BTG2, CCND2, EDNRA, TGFBR3, GJA, LAMA2, RTN4, CDK6, IHH, MAGED1, INSR, CD9, PTGES, TXNIP, ITGB1, SMAD4, MAP3K1, NOTCH2 , IGFBP7, KLF10, KIT, TPM1, PLD1, BTG3, CD47 and MITF. We chose the most regulated genes down the IVM culture, and pointed out those belonging to two ontological groups.Increased expression of the described genes before IVM maturation may indicate the important role of these genes in the process of ovum maturation. After the maturation process, the proteins produced by them did not play such an important role. In summary, the study provides us with many genes that can serve as molecular markers of oocyte processes associated with in vitro maturation. This knowledge can be used for detailed studies on the regulation of oocyte maturation processes.Running title: Genes regulating cellular migration and proliferation in porcine oocytes

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“…9 STRING-generated interaction network between genes that belongs to the "cellular response to hormone stimulus" and "cellular response to unfolded protein". The intensity of the edges reflects the strength of the interaction score on gene expression as compared to EGR1 (Celichowski et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration

Chermuła

Ješeta

Sujka-Kordowska

et al. 2020

Histochem Cell Biol

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Genes influencing oocyte maturation may be valuable for predicting their developmental potential, as well as discerning the mechanistic pathways regulating oocyte development. In the presented research microarray gene expression analysis of immature and in vitro matured porcine oocytes was performed. Two groups of oocytes were compared in the study: before (3 × n = 50) and after in vitro maturation (3 × n = 50). The selection of viable oocytes was performed using the brilliant cresyl blue (BCB) test. Furthermore, microarrays and RT-qPCR was used to analyze the transcriptome of the oocytes before and after IVM. The study focused on the genes undergoing differential expression in two gene-ontology groups: "Cellular response to hormone stimulus" and "Cellular response to unfolded protein", which contain genes that may directly or indirectly be involved in signal transduction during oocyte maturation. Examination of all the genes of interest showed a lower level of their expression after IVM. From the total number of genes in these gene ontologies ten of the highest change in expression were identified: FOS, ID2, BTG2, CYR61, ESR1, AR, TACR3, CCND2, EGR2 and TGFBR3. The successful maturation of the oocytes was additionally confirmed with the use of lipid droplet assay. The genes were briefly described and related to the literature sources, to investigate their potential roles in the process of oocyte maturation. The results of the study may serve as a basic molecular reference for further research aimed at improving the methods of oocyte in vitro maturation, which plays an important role in the procedures of assisted reproduction.

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“Positive Regulation of RNA Metabolic Process” Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach

Cited by 13 publications

References 41 publications

Protein oligomerization is the biochemical process highly up-regulated in porcine oocytes before in vitro maturation (IVM)

Protein oligomerization is the biochemical process highly up-regulated in porcine oocytes before in vitro maturation (IVM)

Analysis of expression of genes responsible for regulation of cellular proliferation and migration – microarray approach based on porcine oocyte model

Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration

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