Positive Response of Isolated Rat Pancreatic Islets to IMOD; Hopes for Better Transplant Outcome and Graft Function

Larijani, Bagher; Salimi, Mina; Pourkhalil, Nazila; Mohammadir, Azadeh; Baeeri, Maryam; Nili-Ahmad, A.; Abdollahi, Mohammad

doi:10.3923/ajava.2011.1019.1025

Cited by 21 publications

(2 citation statements)

References 6 publications

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“…IMOD with its mechanism of action has been found useful in oxidative-stress-related disorders and immunoinflammatory-based diseases like type 1 diabetes [18], colitis [19], pancreatic Langerhans islet transplant [20] and polycystic ovary syndrome [21]. In addition, IMOD has been found safe in preclinical [22] and clinical studies [23] that favors its advantages if supplemented chronically to those getting old.…”

Section: Discussionmentioning

confidence: 99%

Effects of IMOD™ and Angipars™ on mouse D-galactose-induced model of aging

et al. 2012

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The aim of this study was to evaluate the effects of two registered herbal drugs called IMOD and Angipars on mouse model. Aging was induced by D-galactose (500 mg/kg) administered to animals for 6 weeks through drinking water. Male BALB/c mice were randomly divided into 5 groups receiving D-galactose (D-galactose, 500 mg/kg) for 6 weeks; positive control (D-galactose [500 mg/kg] for 6 weeks + Vitamin E [200 mg/kg/day] intraperitoneally for 4 weeks); IMOD (D-galactose [500 mg/kg] for 6 weeks + IMOD [20 mg/kg/day] intraperitoneally for 4 weeks), Angipars (D-galactose [500 mg/kg] for 6 weeks + Angipars [2.1 mg/kg/day] by gavage for 4 weeks); and the fifth group that was sham and not given D-galactose. At the end of treatment, pro-inflammatory markers including tumor necrosis factor-α (TNF-α), interlukine-1β (IL-β), interlukine-6 (IL-6), Nuclear Factor-kappaB (NF-κb), total antioxidant power (TAP), lipid peroxides (LPO) and male sex hormones i.e. testosterone and dehydroepiandrosterone-sulfate (DHEA-S) were measured in the blood.Results showed that D-Galactose induces a significant oxidative stress and proinflammatory cascade of aging while both IMOD and Angipars recovered all of them. Interestingly, IMOD and Angipars were better than Vitamin E in improving male sex hormones in aged mice. This effect is so important and should be considered as an advantage although it cannot be explained with current knowledge. The conclusion is that IMOD and Angipars have marked anti-aging effect on D-galactose-induced model of aging.

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Section: Discussionmentioning

confidence: 99%

Effects of IMOD™ and Angipars™ on mouse D-galactose-induced model of aging

et al. 2012

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“…2 To overwhelm oxidative stress during islet transplantation, various antioxidants like vitamin supplements, trace elements and drugs have been widely examined. 2 –4 On the basis of evidences, we recently hypothesized that the combination of cerium oxide (CeO 2 ) and yttrium oxide (Y 2 O 3 ) nanoparticles with a potential free radical scavenger behavior should be useful to make isolated islets survive until transplanted. 5 Therefore, in the present work, we studied the protective effects of this combination in isolated islets by measuring the biomarkers involved in the survival of islets in an ex vivo model.…”

Section: Introductionmentioning

confidence: 99%

Antiapoptotic effects of cerium oxide and yttrium oxide nanoparticles in isolated rat pancreatic islets

et al. 2013

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Type I diabetes mellitus is a metabolic disease caused by the impairment of pancreatic β-cells mainly mediated through oxidative stress and related apoptosis. Islets transplantation seems a promising treatment for these patients, but during islets transplant, various types of stresses related to the isolation and transplantation procedure compromise the function and viability of islets. We recently hypothesized that the combination of cerium oxide (CeO2) and yttrium oxide (Y2O3) nanoparticles with a potential free radical scavenger behavior should be useful to make isolated islets survive until transplanted. In the present study, oxidative stress-induced apoptosis in isolated rat pancreatic islets exposed to hydrogen peroxide (H2O2) and the protective effects of CeO2 and Y2O3 nanoparticles were investigated. Exposure of islets to H2O2 (50 µm, 2 h) increased intracellular oxidant formation such as reactive oxygen species and subsequently apoptosis and decreased viability, glucose-induced adenosine triphosphate (ATP) production and glucose-stimulated insulin secretion. Pretreatment with CeO2 and/or Y2O3 nanoparticles reduced the oxidant formation and apoptosis and increased viability, glucose-induced ATP production and glucose-stimulated insulin secretion. These results suggest that this combination may protect β-cell apoptosis by improving the oxidative stress-mediated apoptotic pathway.

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Zinc Oxide Nanoparticles Reduce Apoptosis and Oxidative Stress Values in Isolated Rat Pancreatic Islets

Shoae-Hagh

Rahimifard

Navaei-Nigjeh

et al. 2014

Biol Trace Elem Res

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Although toxic effects of zinc oxide nanoparticles (ZnO NPs) have been previously studied, there are still controversies in terms of dose, size, shape, and affecting cells. By such a perspective, in this study, small size of ZnO NPs with a diameter of 10 nm at low concentrations was studied for any effect on the viability and function of isolated rat pancreatic islets. Islets of Langerhans were isolated and assessed for viability, functionality (insulin secretion), cytosolic reactive oxygen species (ROS), and apoptosis by flow cytometry. The LC50 of ZnO NPs was found at 1,400 ng/mL at the first phase of the study. A meaningful increase in viability of islets and insulin secretion in basal and even stimulated concentrations of glucose was found by ZnO NPs (70 ng/mL) with p < 0.001 and p < 0.05, respectively. Likewise, ZnO NPs in 70 ng/mL concentration decreased cytosolic ROS generation (p < 0.05). In the meantime, the percentage of early stage of apoptotic cells dropped down to 17 % (from 29 % of control). These results for the first time confirm that ZnO NPs are not only safe when used at dose of 70 ng/mL but also improve viability and function of pancreatic islets and meanwhile reduce oxidative stress and prevent cells from entering the apoptotic phase.

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Positive Response of Isolated Rat Pancreatic Islets to IMOD; Hopes for Better Transplant Outcome and Graft Function

Cited by 21 publications

References 6 publications

Effects of IMOD™ and Angipars™ on mouse D-galactose-induced model of aging

Effects of IMOD™ and Angipars™ on mouse D-galactose-induced model of aging

Antiapoptotic effects of cerium oxide and yttrium oxide nanoparticles in isolated rat pancreatic islets

Zinc Oxide Nanoparticles Reduce Apoptosis and Oxidative Stress Values in Isolated Rat Pancreatic Islets

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