Positively charged dextran resin inhibits trabecular bone repair in the rabbit tibial physis

Cobos, J.A.; Yang, Jinping; Zhang, Renwen; Krukowski, Marilyn; Simmons, David J.

doi:10.1002/(sici)1097-4636(19980305)39:3<458::aid-jbm16>3.0.co;2-6

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…In all the groups, no phagocytic reaction with multinucleated giant cells was found in the gap areas 7 days after implantation. Because in almost all reports of histologic investigations concerning the charged surfaces of polymer beads, the inflammatory reaction was dominant on day 7, regardless of the polarity and the magnitude of the charges, 4,6,17,18 it is difficult to compare the present results of the charged HAp surfaces with previous studies on charged polymers. At 14 days, the newly formed bone layers directly bonding to the N-surfaces had thickened 2-3 times compared with their thickness on day 7 [ Fig.…”

Section: Cell Reactions To Charged Hap Ceramic Surfacesmentioning

confidence: 68%

“…Cobos et al reported, based on implantation tests using rabbit physis for 1 to 10 weeks, that positively charged dextran resin inhibits trabecular bone repair. 17 Cherico et al studied the effect of electrically charged GTAM on bone formation using rabbit calvarial defects and reported that negatively charged GTAM membranes accelerate and maintain bone neogenesis for terms of 5 days-20 weeks and that positively charged membranes show the lowest new bone formation. 18 These suppressive effects of the positively charged polymer surfaces on bone formation are contrary to the present results.…”

Section: Bone Formation In the Vicinity Of The Positively Charged Surmentioning

confidence: 99%

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Extended bioactivity in the proximity of hydroxyapatite ceramic surfaces induced by polarization charges

Kobayashi

Yamashita

2002

J. Biomed. Mater. Res.

103

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Surface charges of electrically polarized hydroxyapatite (HAp) ceramics were demonstrated to enhance osteoconductivity in the wide gap of the canine bone whereas the bone formation processes varied according to the charge polarity. Cell reactions in the vicinity of the charged surfaces of HAp ceramics were not phagocytic absorption but rather bone formation by stimulated osteoblasts surrounding newly formed bone 7 days after implantation. The bone formation in direct contact with negatively charged ceramic surfaces suggests that the negative charges enhanced the osteobonding ability of the HAp ceramics. In the vicinity of the positively charged HAp surface, the formation of bones derived from osteoid tissues entirely occupied the 0.2-0.3-mm gaps between the HAp and the bone at 7 days. Surface charges induced by electrical polarization significantly cooperated with the innate bioactivity of HAp and reconstructed the wide bone defects more promptly than did the nonpolarized HAp ceramic surface.

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Section: Cell Reactions To Charged Hap Ceramic Surfacesmentioning

confidence: 68%

Section: Bone Formation In the Vicinity Of The Positively Charged Surmentioning

confidence: 99%

Extended bioactivity in the proximity of hydroxyapatite ceramic surfaces induced by polarization charges

Kobayashi

Yamashita

2002

J. Biomed. Mater. Res.

103

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“…The primary interaction between a biomaterial and the surrounding bone involves the outermost molecular layers of the implant [2]. Thus, the surface micro-roughness [3,4], surface energy [5,6], and surface charge [7,8] of the biomaterial play important roles in influencing cellular response.…”

Section: Introductionmentioning

confidence: 99%

Use of polyelectrolyte thin films to modulate Osteoblast response to microstructured titanium surfaces

et al. 2012

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The microstructure and wettability of titanium (Ti) surfaces directly impact osteoblast differentiation in vitro and in vivo. These surface properties are important variables that control initial interactions of an implant with the physiological environment, potentially affecting osseointegration. The objective of this study was to use polyelectrolyte thin films to investigate how surface chemistry modulates response of human MG63 osteoblast-like cells to surface microstructure. Three polyelectrolytes, chitosan, poly(l-glutamic acid), and poly(l-lysine), were used to coat Ti substrates with two different microtopographies (PT, Sa = 0.37 µm and SLA, Sa = 2.54 µm). The polyelectrolyte coatings significantly increased wettability of PT and SLA without altering micron-scale roughness or morphology of the surface. Enhanced wettability of all coated PT surfaces was correlated with increased cell numbers whereas cell number was reduced on coated SLA surfaces. Alkaline phosphatase specific activity was increased on coated SLA surfaces than on uncoated SLA whereas no differences in enzyme activity were seen on coated PT compared to uncoated PT. Culture on chitosan-coated SLA enhanced osteocalcin and osteoprotegerin production. Integrin expression on smooth surfaces was sensitive to surface chemistry, but microtexture was the dominant variable in modulating integrin expression on SLA. These results suggest that surface wettability achieved using different thin films has a major role in regulating osteoblast response to Ti, but this is dependent on the microtexture of the substrate.

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Bovine osteoblasts cultured on polyanionic collagen scaffolds: An ultrastructural and immunocytochemical study

et al. 2012

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Collagen is the most abundant protein in the body and is also the most important component of the extracellular matrix. Collagen has several advantages as a biomaterial such as lack of toxicity, biocompatibility, biodegradability, and easy reabsorption. In this study, we examined bovine osteoblasts cultured on native or anionic collagen scaffolds prepared from bovine pericardium after selective hydrolysis of glutamine and asparagine side chain amides for periods from 24 (BP24) and 48 h (BP48). The cells were cultured in control and mineralization medium at 37 °C in the presence of 5% CO(2). Transmission and scanning electron microscopy, energy dispersive spectroscopy, and an immunocytochemical marker were used for analysis. Cells with an irregular morphology forming a confluent multilayer were observed on matrices kept in control medium. Most of these cells presented a polygonal or elongated flattened morphology. Several spherical deposits of calcium crystal associated with phosphorus were observed on the native and BP48 matrices. Similar results were observed in samples kept in control medium except with lower calcium/phosphorus ratio. Vesicles actively expelled from the cell membrane were also seen (do this vesicles corresponds to calcium/phosphorus deposits). Osteocalcin was clearly visible on matrices kept in mineralization medium and was more expression on the surface of BP48 matrices. The results showed that anionic collagen is able to support osteoblastic differentiation, regardless of the medium used. Finally, the BP48 matrix promoted better osteoblast differentiation than the native matrix.

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Positively charged dextran resin inhibits trabecular bone repair in the rabbit tibial physis

Cited by 4 publications

References 15 publications

Extended bioactivity in the proximity of hydroxyapatite ceramic surfaces induced by polarization charges

Extended bioactivity in the proximity of hydroxyapatite ceramic surfaces induced by polarization charges

Use of polyelectrolyte thin films to modulate Osteoblast response to microstructured titanium surfaces

Bovine osteoblasts cultured on polyanionic collagen scaffolds: An ultrastructural and immunocytochemical study

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