Positron Emission Tomography-Computed Tomography and Magnetic Resonance Imaging Assessments in a Mouse Model of Implant-Related Bone and Joint Staphylococcus aureus Infection

Aguilera-Correa, John Jairo; Salinas, Belén; González-Arjona, Mario; Pablo, D. de; Muñoz, Patricia; Bouza, Emilio; Aceñero, María Jesús Fernández; Esteban, Jaime; Desco, Manuel; Cussó, Lorena

doi:10.1128/spectrum.04540-22

Cited by 2 publications

(1 citation statement)

References 36 publications

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“…An infective osteoarthritis model employs female RjOrl:SWISS (CD1) mice ( n = 4, 30-32 g, 11 weeks old). This model was induced following the methodology described by Aguilera-Correa et al [ 36 ]. Briefly, female RjOrl:SWISS (CD1) mice ( n = 4, 30-32 g, 11 weeks old) underwent surgery which consisted of inserting a 1 cm long resorbable implant (Biosyn 0; Synature) into the medullary cavity of the right femur through the joint.…”

Section: Methodsmentioning

confidence: 99%

In Vivo Detection of Staphylococcus aureus Infections Using Radiolabeled Antibodies Specific for Bacterial Toxins

González,

González-Arjona,

Cussó

et al. 2024

International Journal of Biomedical Imaging

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Purpose. The Gram-positive Staphylococcus aureus bacterium is one of the leading causes of infection in humans. The lack of specific noninvasive techniques for diagnosis of staphylococcal infection together with the severity of its associated complications support the need for new specific and selective diagnostic tools. This work presents the successful synthesis of an immunotracer that targets the α-toxin released by S. aureus. Methods. [89Zr]Zr-DFO-ToxAb was synthesized based on radiolabeling an anti-α-toxin antibody with zirconium-89. The physicochemical characterization of the immunotracer was performed by high-performance liquid chromatography (HPLC), radio-thin layer chromatography (radio-TLC), and electrophoretic analysis. Its diagnostic ability was evaluated in vivo by positron emission tomography/computed tomography (PET/CT) imaging in an animal model of local infection-inflammation (active S. aureus vs. heat-killed S. aureus) and infective osteoarthritis. Results. Chemical characterization of the tracer established the high radiochemical yield and purity of the tracer while maintaining antibody integrity. In vivo PET/CT image confirmed the ability of the tracer to detect active foci of S. aureus. Those results were supported by ex vivo biodistribution studies, autoradiography, and histology, which confirmed the ability of [89Zr]Zr-DFO-ToxAb to detect staphylococcal infectious foci, avoiding false-positives derived from inflammatory processes. Conclusions. We have developed an immuno-PET tracer capable of detecting S. aureus infections based on a radiolabeled antibody specific for the staphylococcal alpha toxins. The in vivo assessment of [89Zr]Zr-DFO-ToxAb confirmed its ability to selectively detect staphylococcal infectious foci, allowing us to discern between infectious and inflammatory processes.

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Section: Methodsmentioning

confidence: 99%

In Vivo Detection of Staphylococcus aureus Infections Using Radiolabeled Antibodies Specific for Bacterial Toxins

González,

González-Arjona,

Cussó

et al. 2024

International Journal of Biomedical Imaging

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Disability-adjusted life years from bone and joint infections associated with antimicrobial resistance: an insight from the 2019 Global Burden of Disease Study

Lewandrowski,

da Silva,

Elfar

et al. 2024

International Orthopaedics (SICOT)

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Positron Emission Tomography-Computed Tomography and Magnetic Resonance Imaging Assessments in a Mouse Model of Implant-Related Bone and Joint Staphylococcus aureus Infection

Abstract: Our results encourage future investigations on the utility of the model for testing different therapeutic procedures for osteomyelitis.

Cited by 2 publications

References 36 publications

In Vivo Detection of Staphylococcus aureus Infections Using Radiolabeled Antibodies Specific for Bacterial Toxins

In Vivo Detection of Staphylococcus aureus Infections Using Radiolabeled Antibodies Specific for Bacterial Toxins

Disability-adjusted life years from bone and joint infections associated with antimicrobial resistance: an insight from the 2019 Global Burden of Disease Study

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