Positron‐emitting tracer imaging of fluoride transport and distribution in tea plant

Niu, Huiliang; Peng, Chuanyi; Zhu, Xudong; Dong, Yangyang; Li, Yeyun; Tang, Ling‐ling; Wan, Xiaochun; Cai, Hao

doi:10.1002/jsfa.10367

Cited by 7 publications

(6 citation statements)

References 30 publications

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“…Root application of the bolus confirmed the ability of [ 18 F]fluoride to enter the transpiration stream through the root tissue bypassing the endodermis. This was consistent with other studies where [ 18 F]fluoride supplied to the roots of tea (Niu et al 2020 ) and cowpea (Furukawa et al 2001 ) that also showed upward mobility into the stem and leaves. The observation that the fluoride entered both WT and fex with equally efficiency (WT: 2.9 1.4 (μCi/g)/μCi bolus, fex : 2.2 0.9 (μCi/g)/μCi bolus), suggested that the main role of FEX was not to keep fluoride out of the root tissue.…”

Section: Resultssupporting

confidence: 93%

“…The source buffer showed negligible loss of radioactivity after administration. These results were consistent with observations made with larger plants which established that movement of [ 18 F]fluoride bolus within plant tissues could be monitored by PET ( Glycine max , McKay et al 1988 ; rice, Kang et al 2009 ; Brassica oleracea , Converse et al 2015 ; tea, Niu et al 2020 ).…”

Section: Resultssupporting

confidence: 89%

“…The initial velocity of fluoride movement within Arabidopsis was slower than the rate of 36 cm/min originally calculated in soybean by McKay et al ( 1988 ), the same as 2.2 cm/min reported for Brassica oleracea (Converse et al 2015 ), and faster than tea (Niu et al 2020 ). The speed was similar to that (1.9–1.1 cm/min) calculated for [ 15 O]H 2 O in rice leaves (Kiyomiya et al 2001 ).…”

Section: Resultssupporting

confidence: 57%

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Fluoride transport in Arabidopsis thaliana plants is impaired in Fluoride EXporter (FEX) mutants

Tausta,

Fontaine,

Hillmer

et al. 2024

Plant Mol Biol

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Fluoride is an environmental toxin prevalent in water, soil, and air. A fluoride transporter called Fluoride EXporter (FEX) has been discovered across all domains of life, including bacteria, single cell eukaryotes, and all plants, that is required for fluoride tolerance. How FEX functions to protect multicellular plants is unknown. In order to distinguish between different models, the dynamic movement of fluoride in wildtype (WT) and fex mutant plants was monitored using [18F]fluoride with positron emission tomography. Significant differences were observed in the washout behavior following initial fluoride uptake between plants with and without a functioning FEX. [18F]Fluoride traveled quickly up the floral stem and into terminal tissues in WT plants. In contrast, the fluoride did not move out of the lower regions of the stem in mutant plants resulting in clearance rates near zero. The roots were not the primary locus of FEX action, nor did FEX direct fluoride to a specific tissue. Fluoride efflux by WT plants was saturated at high fluoride concentrations resulting in a pattern like the fex mutant. The kinetics of fluoride movement suggested that FEX mediates a fluoride transport mechanism throughout the plant where each individual cell benefits from FEX expression.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 57%

See 1 more Smart Citation

Fluoride transport in Arabidopsis thaliana plants is impaired in Fluoride EXporter (FEX) mutants

Tausta,

Fontaine,

Hillmer

et al. 2024

Plant Mol Biol

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“…Most fluoride taken up by tea roots is readily transported through the xylem as F − and F–Al complexes to the leaves, 42 where it is retained in the cell wall or vacuole in the form of F–Al complexes, 43–45 or less prevalent F–Ca complexes, 45 consistent with other hyperaccumulator plants 46,47 . Furthermore, the exogenous addition of calcium and amendments (charcoal or bamboo charcoal) significantly decreases fluoride concentration in tea leaves through reducing the amount of available fluoride in soil, staying in the roots as F–Ca complexes or affecting the properties of leaf cell wall and membrane permeability 48,49 …”

Section: Absorption Transportation and Accumulation Of Fluoride In mentioning

confidence: 99%

Fluoride absorption, transportation and tolerance mechanism in Camellia sinensis, and its bioavailability and health risk assessment: a systematic review

Peng

Ren

et al. 2020

J Sci Food Agric

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Tea is the one of the most popular non-alcoholic caffeinated beverages in the world. Tea is produced from the tea plant (Camellia sinensis (L.) O. Kuntze), which is known to accumulate fluoride. This article systematically analyzes the literature concerning fluoride absorption, transportation and fluoride tolerance mechanisms in tea plants. Fluoride bioavailability and exposure levels in tea infusions are also reviewed. The circulation of fluoride within the tea plantation ecosystems is in a positive equilibrium, with greater amounts of fluoride introduced to tea orchards than removed. Water extractable fluoride and magnesium chloride (MgCl 2) extractable fluoride in plantation soil are the main sources of absorption by tea plant root via active transmembrane transport and anion channels. Most fluoride is readily transported through the xylem as F − /F-Al complexes to leaf cell walls and vacuole. The findings indicate that tea plants employ cell wall accumulation, vacuole compartmentalization, and F-Al complexes to co-detoxify fluoride and aluminum, a possible tolerance mechanism through which tea tolerates higher levels of fluoride than most plants. Furthermore, dietary and endogenous factors influence fluoride bioavailability and should be considered when exposure levels of fluoride in commercially available dried tea leaves are interpreted. The relevant current challenges and future perspectives are also discussed.

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“…Positron emission tomography (PET) is a non-invasive technology for radionuclide imaging, and it is sensitive to and informative of biological processes in vivo . A significant advantage of PET technology is that it is non-invasive, and that this powerful tool has been used not only for the diagnosis of various cancers ( Phelps, 2000 ) but also the study of plant science ( McKay et al, 1988 ; Kang et al, 2009 ; Niu et al, 2020 ), bacterial infections ( Northrup et al, 2019 ; Welling et al, 2019 ) and even the subsurface microbial processes of soils ( Kinsella et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Synthetic Methodologies to Form C-18F Bonds

2022

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Positron emission tomography (PET) is an important technique for the early diagnosis of disease. Due to the specific physical and chemical properties of Fluorine-18, this important isotope is widely used in PET for labelling and molecular imaging, and its introduction into medicine molecules could produce PET tracers. Developing with the development of organic synthetic methodologies, the introduction of Fluorine-18 into drug molecules efficiently and rapidly under mild conditions, and the formation of C-18F chemical bonds, has become one of the leading topics in both organic synthetic chemistry and radiochemistry. In this mini-review, we review a series of recent advances in the organic synthesis of C-18F bonds (2015–2021), including non-catalytic radiofluorinations via good leaving functional groups, transition metal-catalyzed radiofluorinations, and photo- or electro-catalytic synthetic radiofluorinations. As a result of the remarkable advancements in this field, organic synthetic methods for forming C-18F bonds are expected to continue growing.

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Positron‐emitting tracer imaging of fluoride transport and distribution in tea plant

Cited by 7 publications

References 30 publications

Fluoride transport in Arabidopsis thaliana plants is impaired in Fluoride EXporter (FEX) mutants

Fluoride transport in Arabidopsis thaliana plants is impaired in Fluoride EXporter (FEX) mutants

Fluoride absorption, transportation and tolerance mechanism in Camellia sinensis, and its bioavailability and health risk assessment: a systematic review

Recent Advances in Synthetic Methodologies to Form C-18F Bonds

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