Possibilité de diagnostic sérologique macroscopique des leptospiroses à l'aide d'un antigène unique

Mazzonelli, J.; Mailloux, M

doi:10.1016/s0399-077x(74)80135-6

Cited by 10 publications

(2 citation statements)

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“…These cross-reactive antigens could include proteins and/or components of leptospiral LPS (30). Currently available serologic assays include the macroscopic agglutination (31), indirect hemagglutination (28), and microcapsule agglutination (2) tests, all of which are less sensitive than the MAT and identify less than 50% of patients presenting with early-phase leptospirosis. Assays that detect immunoglobulin M (IgM) and are based upon crude antigen (1,14,27,38,44) appear to be more sensitive for serodiagnosis but may be subject to variations in specificity.…”

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confidence: 99%

Leptospiral Proteins Recognized during the Humoral Immune Response to Leptospirosis in Humans

et al. 2001

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Leptospirosis is an emerging zoonosis caused by pathogenic spirochetes belonging to the genus Leptospira. An understanding of leptospiral protein expression regulation is needed to develop new immunoprotective and serodiagnostic strategies. We used the humoral immune response during human leptospirosis as a reporter of protein antigens expressed during infection. Qualitative and quantitative immunoblot analysis was performed using sera from 105 patients from Brazil and Barbados. Sera from patients with other diseases and healthy individuals were evaluated as controls. Seven proteins, p76, p62, p48, p45, p41, p37, and p32, were identified as targets of the humoral response during natural infection. In both acute and convalescent phases of illness, antibodies to lipopolysaccharide were predominantly immunoglobulin M (IgM) while antibodies to proteins were exclusively IgG. Anti-p32 reactivity had the greatest sensitivity and specificity: positive reactions were observed in 37 and 84% of acute-and convalescent-phase sera, respectively, while only 5% of community control individuals demonstrated positive reactions. Six immunodominant antigens were expressed by all pathogenic leptospiral strains tested; only p37 was inconsistently expressed. Two-dimensional immunoblots identified four of the seven infection-associated antigens as being previously characterized proteins: LipL32 (the major outer membrane lipoprotein), LipL41 (a surface-exposed outer membrane lipoprotein), and heat shock proteins GroEL and DnaK. Fractionation studies demonstrated LipL32 and LipL41 reactivity in the outer membrane fraction and GroEL and DnaK in the cytoplasmic fraction, while p37 appeared to be a soluble periplasmic protein. Most of the other immunodominant proteins, including p48 and p45, were localized to the inner membrane. These findings indicate that leptospiral proteins recognized during natural infection are potentially useful for serodiagnosis and may serve as targets for vaccine design.

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confidence: 99%

Leptospiral Proteins Recognized during the Humoral Immune Response to Leptospirosis in Humans

et al. 2001

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“…A titre of 1:80 was considered positive. (11,13,14) MSAT was performed by adding one drop of patient serum and one drop of polyvalent antigen together. A 4+ agglutination titre by MSAT was considered to be a significant titre.…”

Section: Diagnostic Criteriamentioning

confidence: 99%

Clinical Impact of Leptospirosis on Pregnancy: An Analysis

Udawat¹,

Sumathi²,

Nithyalakshmi³

et al. 2014

jemds

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BACKGROUND: Leptospirosis is one of the most widespread zoonotic diseases in the world. Infection may range from subclinical infection to death of the patient, although majority of the cases are mild and self-limited. AIMS & OBJECTIVES: The present study was carried out to determine the clinical impact of leptospirosis in pregnant women attending antenatal clinic of our tertiary care hospital. METHODS & MATERIALS: In this case control study, blood samples from pregnant women who attended routine antenatal checkup during March 2008 to March 2010 were included the study. A total of 33 cases and 237 controls were compared during the study period. The diagnosis was made by performing the two serological tests: (i) The rapid screening test-Macroscopic Slide Agglutination Test (MSAT) and the other (ii) the confirmatory test-Microscopic Agglutination Test (MAT). RESULTS: Out of 270 patients, 33 (17.9%) were found to be positive for leptospirosis by Microscopic Agglutination Test (MAT) and 35 (19%) by Macroscopic Slide Agglutination Test (MSAT).The biochemical parameters like SGPT, SGOT were found to be elevated. Increased awareness among pregnant women is of utmost important for early detection & treatment of the disease and thus, for the safety of the fetus.

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Leptospirosis

Baranton¹

2003

Encyclopedia of Environmental Microbiology

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Bacteriology Physiology—Metabolism Epidemiology Risk Factors (27) Transmission Mechanisms (27) Virulence and Pathogenesis (27) The Disease Control and Treatment of Leptospirosis (15,27)

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Possibilité de diagnostic sérologique macroscopique des leptospiroses à l'aide d'un antigène unique

Cited by 10 publications

References 1 publication

Leptospiral Proteins Recognized during the Humoral Immune Response to Leptospirosis in Humans

Leptospiral Proteins Recognized during the Humoral Immune Response to Leptospirosis in Humans

Clinical Impact of Leptospirosis on Pregnancy: An Analysis

Leptospirosis

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