Possibilities and limitations of using low biomass samples for urologic disease and microbiome research

Kim, Jung Kwon; Song, Sang Hun; Jung, Gyoohwan; Song, Byeongdo; Hong, Sung Kyu

doi:10.1016/j.prnil.2022.10.001

Cited by 6 publications

(1 citation statement)

References 101 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, genome-wide association and candidate gene studies regarding the contribution of polygenic influences from multiple loci have reported that multiple genes and molecular pathways contribute to stone formation. Overall, the pathophysiology of stone formation is currently accepted to have a complex etiology that can involve a monogenic disorder or polygenic traits, dietary and hormonal components, and environmental factors [ [7] , [8] , [9] , [10] ].…”

Section: Introductionmentioning

confidence: 99%

Monogenic features of urolithiasis: A comprehensive review

Koo

Halawani

Wong

et al. 2024

Asian Journal of Urology

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Monogenic features of urolithiasis: A comprehensive review

Koo

Halawani

Wong

et al. 2024

Asian Journal of Urology

View full text Add to dashboard Cite

A prospective evaluation of the prostate microbiome in malignant and benign tissue using transperineal biopsy

Chen,

James,

Khemmani

et al. 2024

The Prostate

View full text Add to dashboard Cite

BackgroundThe link between the prostate microbiome and prostate cancer remains unclear. Few studies have analyzed the microbiota of prostate tissue, and these have been limited by potential contamination by transrectal biopsy. Transperineal prostate biopsy offers an alternative and avoids fecal cross‐contamination. We aim to characterize the prostate microbiome using transperineal biopsy.MethodsPatients with clinical suspicion for prostate cancer who were to undergo transperineal prostate biopsy with magnetic resonance imaging (MRI) fusion guidance were prospectively enrolled from 2022 to 2023. Patients were excluded if they had Prostate Imaging Reporting and Data System lesions with scores ≤ 3, a history of prostate biopsy within 1 year, a history of prostate cancer, or antibiotic use within 30 days of biopsy. Tissue was collected from the MRI target lesions and nonneoplastic transitional zone. Bacteria were identified using 16S ribosomal RNA gene sequencing.ResultsAcross the 42 patients, 76% were found to have prostate cancer. Beta diversity indices differed significantly between the perineum, voided urine, and prostate tissue. There were no beta diversity differences between cancerous or benign tissue, or between pre‐ and postbiopsy urines. There appear to be unique genera more abundant in cancerous versus benign tissue. There were no differences in alpha diversity indices relative to clinical findings including cancer status, grade, and risk group.ConclusionsWe demonstrate a rigorous method to better characterize the prostate microbiome using transperineal biopsy and to limit contamination. These findings provide a framework for future large‐scale studies of the microbiome of prostate cancer.

show abstract

Our Knowledge of the Relationship of the Urinary Microbiome and Overactive Bladder: Past, Present, Future

Jacobsen,

Amin

2023

Curr Bladder Dysfunct Rep

View full text Add to dashboard Cite

Possibilities and limitations of using low biomass samples for urologic disease and microbiome research

Cited by 6 publications

References 101 publications

Monogenic features of urolithiasis: A comprehensive review

Monogenic features of urolithiasis: A comprehensive review

A prospective evaluation of the prostate microbiome in malignant and benign tissue using transperineal biopsy

Our Knowledge of the Relationship of the Urinary Microbiome and Overactive Bladder: Past, Present, Future

Contact Info

Product

Resources

About