Possibilities for Application of Photoautotrophy in Micropropagation of Dzhanka 4 (Prunus Cerasifera Ehrt.) Rootstock

Nacheva, L.; Ivanova, K. P.; Manolov, P.; Zlatev, Z.

doi:10.17660/actahortic.2002.577.33

Cited by 3 publications

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“…Stanley have shown that the production of own-rooted planting material trees have had many advantages over the conventional graft propagation, thus providing an opportunity for rapid restoration of plum orchards with PPV free high-tech cultivars. Positive results in PPV elimination in two plum cultivars, Kyustendilska sinya and Valejevka using the method of clonal micropagation have been obtained (72). The authors have shown the significant importance of number of subcultures for tissue virus elimination and the good opportunity for PPV elimination.…”

Section: Production Of Virus Free Plant Materialsmentioning

confidence: 94%

Sharka Disease in Bulgaria: Past, Present and Future

Kamenova

Milusheva

2005

Biotechnology & Biotechnological Equipment

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This review is an attempt to synthesize almost 70 years investigations of Sharka disease in Bulgaria. The results obtained from first virus discovery, disease dissemination throughout stone fruit growing regions, present virus incidence on different Prunus species, natural hosts of the virus, virus detection, strain characterization, susceptibility of plum, peach and apricot cultivars to Sharka disease and virus control measures applied in the past and today are presented.

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Section: Production Of Virus Free Plant Materialsmentioning

confidence: 94%

Sharka Disease in Bulgaria: Past, Present and Future

Kamenova

Milusheva

2005

Biotechnology & Biotechnological Equipment

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“…[1] where a is maximal CO 2 assimilation (A max ) at saturated zone; b is parameter which is used for the calculation of CO 2 evolved during the dark respiration (R) at A max (R = a + b) (Nacheva et al, 2002); c is constant.…”

Section: Gas Exchange Measurementsmentioning

confidence: 99%

Drought-induced changes and recovery of photosynthesis in two bean cultivars<br>(Phaseolus vulgaris L.)

Zlatev

2013

Emir. J. Food Agric

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The effects of soil drought on photosynthesis and chlorophyll fluorescence in the leaves of two common bean (Phaseolus vulgaris L.) cultivars -Zarya and Tangra were studied, as well as recovery of photosynthesis after re-watering. Drought was imposed 14 days after the emergency by withholding water for 10 days in which soil water potential reached -0.9 MPa. Water stress led to a noticeable decrease in both the initial slope of the A n /C i curve and A max in the primary leaf of the studied cultivars. The most marked reduction in leaf gas exchange was observed in cv. Tangra.  was reduced more than three folds and A max -more than six folds. Exposure of bean plants to soil drought and provoked leaf water deficit resulted in a dramatic reduction (with an 84.17%) of A n at normal ambient CO 2 concentration (C a =370 mol mol -1 ). The lower reduction in leaf gas exchange parameters were observed in cv. Zarya. Drought stress induces an increase of F 0 accompanied by a decrease of F m in the studied cultivars, being cv. Zarya less affected. The F v /F m ratio was significantly decreased in cv. Tangra and only showed a slight tendency to a decrease in cv. Zarya. Cv. Tangra presented a decrease of 56% in qP in the primary leaf, while in cv. Zarya qP decreased with 32%. Accordingly, Y strongly decreased in cv. Tangra, while in cv. Zarya Y was less affected. 3 days after re-watering photosynthesis of cv. Zarya was about 87% from the control plants, while in cv. Tangra photosynthesis was only 68% from control. Chlorophyll fluorescence parameters were recovered to the greater extent. On the basis of the data obtained we could arrange photosynthetic apparatus of cv. Zarya as relatively drought tolerant and that of cv. Tangra as drought sensitive.

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“…Successful in vitro clonal propagation methods are reported in many rootstocks, including plum (Morini et al 1990;Fortuna et al 1996;Nacheva et al 2002;Vujović et al 2007), cherry (Ružić, Cerović 1987;Muna et al 1999;Ružić et al 2003;Sedlák et al 2008) and pear rootstocks (Yeo, Reed 1995;De Paoli et al 2002;Ružić et al 2008). While most of the studies were focused on the influence of nutrient media, including mineral composition, carbohydrates content and type/ concentration of plant growth regulators, the influence of repeated subculturing on shoot multiplicaIn vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks T. Vujović 1 tion and growth received less attention in literature (Grant, Hammat 1999).…”

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confidence: 99%

In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks

Vujović¹,

Ružić²,

Cerović³

2012

Hortic. Sci.

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Vujović T., Ružić Dj., Cerović R., 2012. In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks. Hort. Sci. (Prague), 39: 101-107.In vitro shoots of vegetative rootstocks for cherry (Gisela 5 and Gisela 6), plum (Fereley Jaspi) and pear (Pyrodwarf ) were repeatedly subcultured for 10 subcultures on Murashige and Skoog medium of unchanged hormonal composition. Shoot formation capacity decreased over repeated subculturing in all genotypes. The first significant decrease in multiplication index was observed after first subculture in Gisela 6 and Fereley Jaspi, while in Gisela 5 the decline occurred after second subculture, and remained at that level. As for Gisela 6 and Fereley Jaspi, multiplication index was mainly stable from second to forth subculture, whereupon the second decline in shoot formation was observed. Although Pyrodwarf showed very low multiplication capacity, shoot multiplication slightly increased over the first three subcultures and then declined. This irreversible decline could be due to residual effects of hormones. However, no visible morphological variations or aberrations of shoots were found in successive subcultures in any genotype. Quality of shoots in terms of shoot length varied during subculturing, but the highest quality was observed in later subcultures (from fifth subculture onwards). After subculturing, several media were evaluated for induction of rhizogenesis in order to achieve high rooting rates in tested rootstocks. The highest rooting ability (100%) among genotypes was observed in Fereley Jaspi, followed by Pyrodwarf and Gisela 6 (the best rooting percentage being 90% in both) and Gisela 5 (70%). Rooted shoots were successfully acclimatized under the mist system in greenhouse.

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Possibilities for Application of Photoautotrophy in Micropropagation of Dzhanka 4 (Prunus Cerasifera Ehrt.) Rootstock

Cited by 3 publications

References 0 publications

Sharka Disease in Bulgaria: Past, Present and Future

Sharka Disease in Bulgaria: Past, Present and Future

Drought-induced changes and recovery of photosynthesis in two bean cultivars<br>(Phaseolus vulgaris L.)

In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks

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