Possibilities of extraction and characterization of ancient plasma proteins in archaeological bones

Brandt, Elisabeth; Wiechmann, Ingrid; Grupe, Gisela

doi:10.1127/anthranz/58/2000/85

Cited by 4 publications

(1 citation statement)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Auch Proteine aus Knochen konnten nachgewiesen werden (z.B. Brandt et al 2000, Poinar und Stankiewicz 1999, Tuross und Stathoplos 1993. Sogar in fossilem Material gelang die Bindung spezifischer Proteine im Radioimmunoassay (Lowenstein 1980(Lowenstein , 1991.…”

Section: Endogene Biomoleküleunclassified

Steroidhormone in bodengelagertem Skelettmaterial

Holger¹

View full text Add to dashboard Cite

Biosyntheseweg von Steroidhormonen im menschlichen Körper Abb. 2.1.3.B Chemische Struktur von 17 β-Estradiol Abb. 2.1.3.C Veränderungen der Serum-Konzentrationen wichtiger Sexualhormone einschließlich E2 bei Eintritt in die Menopause Abb. 2.1.3.D Anstieg des E2-Spiegels in Serum während der Schwangerschaft Abb. 3.1.2.A Skizze der archäologischen Ausgrabungsfläche "Hinter den Brüdern" in Goslar. Eingezeichnet sind die ergrabenen Bestattungen, gelb markiert die Individuen, deren Knochen auf Estradiol untersucht wurden Abb. 3.2.1.B Grundriß der Lichtensteinhöhle Abb. 3.2.1.C Gemauerte Gruft der Herzogin Anna von Brandenburg im Mittelschiff der Klosterkirche zu Bordesholm Abb. 3.2.1.D Proximales Ende eines der beiden Ossa metatarsalia von BO 9 Abb. 3.2.1.E Vergleich der beiden Ossa metatarsalia von BO 9.

show abstract

Section: Endogene Biomoleküleunclassified

Steroidhormone in bodengelagertem Skelettmaterial

Holger¹

View full text Add to dashboard Cite

show abstract

How reliable are immunological tools for the detection of ancient proteins in fossil bones?

Brandt

Wiechmann

Grupe

2002

Intl J of Osteoarchaeology

Self Cite

View full text Add to dashboard Cite

100 Pre-Contact human skeletons from six different excavation sites on the Peruvian coast, which showed an excellent state of preservation both on the macro-and the microscopic level, have been investigated for the preservation of three plasma proteins (α 2 -HS-glycoprotein, albumin and α 1 -antitrypsin) by enzyme-linked immunosorbent assay. A subsample of 40 skeletons out of this skeletal series had been analysed for the state of preservation of polymorphic plasma proteins by electrophoretic methods (Brandt et al., 2000), revealing extensive alterations of the target proteins by diagenesis. The question remained, therefore, as to the reliability of common immunological tools for the unambiguous detection and quantification of non-collagenous proteins in archaeological bone. In this study, we succeeded in the immunological detection of three target proteins in the skeletal remains. However, a variety of non-specific reactions were also observed with bone extracts from non-human mammals, some collagen types, and also bacterial cells. This wide range of cross-reactions with antigens not normally tested in the course of the production of commercially available antibodies adds a cautionary note to immunological approaches towards the study of ancient proteins.

show abstract

Molecular paleontology: some current advances and problems

Schweitzer

2004

Annales de Paléontologie

View full text Add to dashboard Cite

Possibilities of extraction and characterization of ancient plasma proteins in archaeological bones

Cited by 4 publications

References 4 publications

Steroidhormone in bodengelagertem Skelettmaterial

Steroidhormone in bodengelagertem Skelettmaterial

How reliable are immunological tools for the detection of ancient proteins in fossil bones?

Molecular paleontology: some current advances and problems

Contact Info

Product

Resources

About