Possibilities of Optimizing the Purity of Mannose in Continuous Chromatographic Separation

Abstract

The development of separation technology based on continuous simulated moving bed chromatography in sugar technology is described. This is a potential economic alternative method both in terms of separation efficiency and process performance, and in terms of the high purity of the resulting products. This work focuses in detail on the isolation of mannose from natural polysaccharides, such as galactomannans; however, the developed procedures and equipment can be further applied to the separation of various liquid mixtures of carbohydrates. The main aim was to find optimal operating conditions for hydrolytic and separation steps of the whole technology. The obtained results provide a basic data set for further development of the industrial application.

Comprehensive Characterization of Micronized Wholemeal Flours: Investigating Technological Properties across Various Grains

“…Sugars were measured by anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) (electrochemical detector ED50, Dionex, Thermo Fisher Scientific), column CarboPac PA1 (2 mm × 250 mm, Dionex, Thermo Fisher Scientific, Sunnyvale, CA, USA), and pump (model GS50, Dionex, Thermo Fisher Scientific, Sunnyvale, CA, USA) providing a flow rate of 0.25 mL/min at 25 °C (thermostat TCC-100 Dionex, Thermo Fisher Scientific). The mobile phase composition was 16 mmol/L, and an aqueous solution of sodium hydroxide was used for isocratic elution (for 20 min), followed by 20 min of column regeneration in 200 mmol/L to obtain the aqueous solution of sodium hydroxide [ 13 ].…”