Studied the content the content of phenolic compounds (flavonols, flavans (catechins), tannins), polysaccharides (pectins, protopectins), tetraterpenes (carotenoids) and antioxidant activity of Cichorium intubus L., Taraxacum officinale Wigg., Arnica iljinii (Maguire) Iljin., Echinops sphaerocephalus L. cultivated in conditions of Western Siberia. A significant part of the biologically active substances are tannins (up 28.25%) and protopectins (up 11.11%). The content of flavonoids in the studied samples of C. intybus plants corresponds to the indicators of chicory grass for other regions of the Russian Federation, including the regions of its industrial cultivation. The flowers and leaves of the studied samples of T. officinale are characterized by approximately the same content of flavonols (1.02–1.43%), pectins (0.80–1.08%) and protopectins (7.94–8.87%). The leaves and flowers of A. iljinii are distinguished by a rather high content of flavonols (up to 4.23%) and tannins (up to 28.25%). In terms of the content of tannins (8.34%), catechins (36.3 mg/%), protopectins (10.06%) and carotenoids (64.60 mg/%), the leaves of E. sphaerocephalus are significantly superior to the flowers. Flavonols are present in equal proportions in leaves (0.68%) and flowers (0.64%). Indicators of the total content of phenolic antioxidants in the leaves and flowers of C. intubus, T. officinale, A. iljinii, E. sphaerocephalus do not differ significantly.