Possibilities to intensify and integrate aqueous two-phase extraction for IgG purification

Mündges, J.; Zierow, Jessica; Langer, Uwe; Zeiner, Tim

doi:10.1016/j.seppur.2015.09.008

Cited by 17 publications

(11 citation statements)

References 30 publications

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“…Previous studies on the purification of this enzyme are mainly focused on highly selective techniques with the final purpose of enzyme characterization instead of enzyme recovery and industrial application [17][18][19]. ATPSs are regarded as a suitable approach to recover and concentrate biomolecules in a single extraction step [48]. Furthermore, these biphasic systems can be successfully used in combination with other separation techniques, generally allowing the scale up of the process with excellent reproducibility [48,49].…”

Section: Partial Purification Of Esterasementioning

confidence: 99%

“…ATPSs are regarded as a suitable approach to recover and concentrate biomolecules in a single extraction step [48]. Furthermore, these biphasic systems can be successfully used in combination with other separation techniques, generally allowing the scale up of the process with excellent reproducibility [48,49]. The few studies reported on esterase purification by ATPS are focused on esterases from plant origin.…”

Section: Partial Purification Of Esterasementioning

confidence: 99%

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Integrated strategy for purification of esterase from Aureobasidium pullulans

Lemes

Silvério

Rodrigues

et al. 2019

Separation and Purification Technology

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Esterases catalyze the cleavage and formation of ester bonds of a broad range of substrates presenting a widespread spectrum of industrial applications. This work aimed to partially purify and characterize an esterase from Aureobasidium pullulans LABIOTEC 01 produced in a culture medium containing olive mill wastewater. Esterase purification was evaluated using different strategies, namely the enzyme recovery by PEG-salt aqueous twophase systems (ATPSs); and the enzyme precipitation with ammonium sulfate, acetone, and ethanol. The best purification factor (18 ± 2) was obtained when the ATPS composed of 20% (w/w) polyethylene glycol (PEG) 6000 and 5.8% (w/w) potassium phosphate buffer (PPB) pH 8.0 was combined with acetone precipitation. The partially purified enzyme presented an optimum pH of 5.0, although it remained active in the pH range of 4.5 to 7.5 (≥ 50% relative activity). The optimum temperature was found to be 60°C. Furthermore, the addition of salts such as FeCl 3 , CuSO 4 and MnCl 2 promoted an increase in the enzymatic activity (above 100%). The enzyme was found to be stable and showed high activity when exposed to polar solvents such as dimethyl sulfoxide, dimethylformamide, and methanol. The use of an integrated strategy of purification combining simple purification methods such as ATPS and precipitation was herein reported for the first time for esterase. This strategy proved to be an interesting approach to partially purify the esterase produced under submerged fermentation by A. pullulans. Furthermore, the enzyme showed potential to be applied in industrial biocatalytic processes using high temperature and different salts or solvents. Also, the production of esterase using olive mill wastewater as substrate demonstrated to be a suitable alternative to reduce and valorize agro-industrial residues.

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Section: Partial Purification Of Esterasementioning

confidence: 99%

Section: Partial Purification Of Esterasementioning

confidence: 99%

Integrated strategy for purification of esterase from Aureobasidium pullulans

Lemes

Silvério

Rodrigues

et al. 2019

Separation and Purification Technology

View full text Add to dashboard Cite

show abstract

“…Depending on the biological source, different bioproducts can be easily recovered and even purified by a proper choice of the ABS. As an example, it is possible to extract monoclonal antibodies, antibodies and viruses from CHO cells or monoclonal antibodies, , and antibodies, from hybridoma cells. From plants and plant cells, it is also possible to recover monoclonal antibodies, as well as pigments,, enzymes, and other proteins , , .…”

Section: Introductionmentioning

confidence: 99%

Liquid–liquid extraction of biopharmaceuticals from fermented broth: trends and future prospects

Santos

Santos-Ebinuma

Pessoa

et al. 2017

J of Chemical Tech & Biotech

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Biopharmaceuticals are one of the most important groups of biotechnological products, representing one-quarter of all pharmaceutical sales and providing suitable and efficient medical care for many previously untreatable diseases. However, their production and purification usually require complex processes, resulting in extremely expensive final products. Thus, the development of newer, simpler and cheaper methods for biosynthesis and purification of these biocompounds, as well as proper integration between these stages, are crucial to reduce their commercial costs and to increase the scale of biopharmaceuticals' medical use. One solution for this concern relies on the proper integration between upstream and downstream processing applying liquid-liquid extraction systems as an intermediate stage. Recently, several works reported that a proper choice of liquid-liquid systems such as aqueous biphasic systems (ABS) can be a suitable platform to simplify, reduce the number of the downstream stages, improve the recovery and purification yields and, consequently, decrease biopharmaceuticals' manufacturing costs. This review will explain the general concept of biopharmaceuticals, their main production and purification methods, particularly, exploring the use of ABS as effective and integrative platforms for their recovery and purification, and providing further insights into the future trends and prospects in the field.

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“…, in recent years, compounds such as low molecular weight alcohols, surfactants, and ionic liquids have also been used in the formation of ATPS . The main advantages of this methodology include a very fast to reach partition equilibrium, low cost, low toxicity and recyclability of the raw materials, scale‐up potential, easiness of process integration, capability of continuous application leading to a reduction of process time and costs at increasing yields, and a high biocompatibility with the molecules being separated …”

Section: Introductionmentioning

confidence: 99%

“…). Nowadays, ATPS are being used extensively for the recovery of valuable biopharmaceuticals such as monoclonal antibodies, growth factors and hormones among others.…”

Section: Introductionmentioning

confidence: 99%

Novel aspects and future trends in the use of aqueous two‐phase systems as a bioengineering tool

González‐Valdez

Mayolo‐Deloisa

Rito‐Palomares

2017

J of Chemical Tech & Biotech

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Traditionally, aqueous two-phase systems (ATPS) have been used as a liquid-liquid extraction technique for the primary recovery and purification of biological samples. The enormous potential of their usage comes with great economical and technical advantages mainly due to the mild physicochemical environment. Nowadays, the use of ATPS as a bioengineering technique is approaching an era where new possibilities are being explored to maximize their use and implementation in the development of novel practical applications and tools. In this context, 'intelligent' polymers are being used as phase forming chemicals in ATPS in route to process integration. Extractive fermentation in ATPS is being re-evaluated with the aim of effectively growing microorganisms while recovering their fermentation products in different phases. ATPS are also being used as a tool for refolding of proteins. There are also several innovative efforts being made towards implementing this bioengineering tool as a continuous process moving away from traditional batch operations. In general, the possibilities of implementing ATPS in different bioprocessing aspects are growing considerably and gaining importance. This review aims to present the novel trends in the use and development of ATPS strategies as complete bioengineering tools and to provide a full perspective of their possibilities in the near future.

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Possibilities to intensify and integrate aqueous two-phase extraction for IgG purification

Cited by 17 publications

References 30 publications

Integrated strategy for purification of esterase from Aureobasidium pullulans

Integrated strategy for purification of esterase from Aureobasidium pullulans

Liquid–liquid extraction of biopharmaceuticals from fermented broth: trends and future prospects

Novel aspects and future trends in the use of aqueous two‐phase systems as a bioengineering tool

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