Possibility of culturing foetal cells at early stages of pregnancy

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Chromosome studies in 172 cases 01 induced abortions are reported. The material comprising 212 cultures war obtained from fetal skin, placenta, amnion, mid amniotic fluid cells. Six fetuses with aberrations (3.48 %) were detected, namely two 47,XX, +C, one 47,XX,+E, one 47,XY,+G, one. 46,XX/47,XX,+C, and one 46,XX/47,XX, +G. The average maternal age for the total material was 26.5 years and for the aberrations 33.6 years. The average gestational age for the total material was 12 1/2 weeks and for the aberrations 10 1/2 weeks. The sex ratio was 0.88 for the abortions of the first trimester and 1.30 for those of the second trimester.

Section: Methodsmentioning

confidence: 99%

Chromosome studies in induced abortions

Hahnemann¹

1973

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“…Details of culturing and harvesting techniques for amniotic fluid cells have been described in a previous report (Hahnemann 1972). The other tissues were cultured by the same method, but after initial preparation of a cell suspension by mincing and trypsinization.…”

Section: Methodsmentioning

confidence: 99%

Antenatal genetic diagnosis in a kindred with a 15p+ chromosome

Hahnemann¹,

Eiberg²

1973

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Karyotype studies arc reported in 15 members of a kindred, a 15p+ marker chromosome being present in six of the members, including the proband as well as her mother and aunt. The proband was a Down's syndrome patient with a regular trisomy‐21 besides the marker chromosome. The marker chromosome could represent a Y/15 translocation, as judged from morphology and an intensive fluorescence of the extra material, or it could possibly be interpreted as a giant satellite. In relation to a case of genetic counseling in the aunt, including prenatal diagnosis, the question whether the marker chromosome may imply an increased risk of pathologic offspring is considered.

“…The optimal time for amniocentesis is about the 15th. week of pregnancy (Valenti & Kehaty 1969, Nadler & Gerbie 1970, Ferguson-Smith et al 1971, Milunsky et al 1972, Hahnemann 1972. Diagnostic amniocentesis earlier in pregnancy has seldom been attempted.…”

mentioning

confidence: 99%

Early prenatal diagnosis; A study of biopsy techniques and cell culturing from extraembryonic membranes

Hahnemann¹

1974

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The study concerns the diagnosis of fetal condition early in pregnancywithin the first trimester. An instrument was designed to allow biopsy of the extraembryonic membranes under visual control. The instrument was tested in 95 pregnant women prior to legal termination of pregnancy. In 28 cases, the biopsies were taken in the outpatient clinic and the patients were observed for 8 d before the pregnancy was terminated. Thirty-eight per cent of the biopsies were successful. Causes of failure were puncture or biopsy of the amniotic membrane, and bleeding which obscured vision. Biopsies were nonetheless obtained in all but four cases, and 60% contained chorionic villi. Target biopsy of chorion as chorionic villi is, however, possible, and the optimal time seems to be in the tenth week of pregnancy. Culture of biopsy material and karyotyping was successful in virtually all instances. When fetal tissue was identified in the abortion products, and cultured after subsequent termination (24 instances) karyotype determination confirmed the finding from biopsy culture cells. No maternal complications followed the procedure. The possibility is discussed that the present approach may be developed for clinical use as an alternative to transabdominal amniocentesis in the 15th. week.