Possibility of uncoupling protein 1 expression in bovine fast-twitch muscle          fibers

Diao, Zhicheng; Shimokawa, Fumie; YOSHIOKA, Hidetugu; Itoyama, Erina; Matsumura, Manami; Murakami, Masaru; KITAMURA, Shoko; NAGASE, Hiroshi; Matsui, Tohru; FUNABA, Masayuki

doi:10.1292/jvms.23-0057

The Journal of Veterinary Medical Science

2023

DOI: 10.1292/jvms.23-0057

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Possibility of uncoupling protein 1 expression in bovine fast-twitch muscle fibers

Zhicheng Diao

Fumie Shimokawa

Hidetugu YOSHIOKA

et al.

Abstract: Uncoupling protein 1 (UCP1) is responsible for non-shivering thermogenesis in brown/beige adipocytes in humans and rodents. Previously, we showed unexpected expression of UCP1 in bovine skeletal muscles. Here we evaluated Ucp1 mRNA levels in the muscle tissue of Japanese Black steers. Expression of Ucp1 was higher in 30-month-old cattle than in 26-month-old cattle. Levels of myosin heavy chain (Myh)1, an MYH predominantly expressed in fast-twitch muscles, were also significantly higher in cattle aged 30 months… Show more

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2024

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A possibility of uncoupling protein 1 induction with the enhancement of myogenesis related to ruminal fermentation

Diao,

Jia,

Itoyama

et al. 2024

Sci Rep

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The expression of uncoupling protein 1 (UCP1), which regulates energy expenditure, is limited to brown/beige adipocytes in most mammals; however, it is also detected in the skeletal muscles of cattle. We previously observed a positive relationship between Ucp1 and fast-twitch myosin heavy chain ( Myh ) expression in bovine skeletal muscles. In the present study, we explored the regulatory expression of Ucp1 in bovine myogenic cells using cell culture. Vitamin C and high-dose capsaicin, which induce the formation of fast-twitch myotubes in murine myogenic cells, did not stimulate myogenesis in bovine myosatellite cells. Treatment with 4-phenylbutyric acid (PBA), a histone deacetylase inhibitor that enhances histone acetylation, upregulates the expression of all myogenic regulatory factors (MRFs), except Myog , in bovine myogenic cells. Consistent with this, PBA increased the expression levels of acetylated lysine 27 of histone 3 (H3K27), the fast-twitch component MYH1/2, and Ucp1 in bovine myogenic cells. SB203580, an inhibitor of p38 MAP kinase, blocked PBA-induced myogenesis and Ucp1 upregulation. PBA is a butyric acid-related molecule, and cattle produce large amounts of volatile fatty acids (VFAs), including acetic acid, propionic acid, and butyric acid, through ruminal fermentation. Propionic acid treatment stimulated H3K27 acetylation, myogenesis, and Ucp1 induction. Thus, the upregulation of muscular Ucp1 may be related to myogenic stimulation through the modulation of histone acetylation status in cattle; we propose that the cattle-specific expression of muscular UCP1 results from VFA production through ruminal fermentation.

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A possibility of uncoupling protein 1 induction with the enhancement of myogenesis related to ruminal fermentation

Diao,

Jia,

Itoyama

et al. 2024

Sci Rep

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Differential Expression of Skeletal Muscle Sites and Fast and Slow Muscle Fibers in Mongolian Horses

Jia,

Bou,

Ding

et al. 2024

Russ J Genet

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Research Progress on the Regulating Factors of Muscle Fiber Heterogeneity in Livestock: A Review

Wang,

Zhang,

Liu

2024

Animals

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The type of muscle fiber plays a crucial role in the growth, development, and dynamic plasticity of animals’ skeletal muscle. Additionally, it is a primary determinant of the quality of both fresh and processed meat. Therefore, understanding the regulatory factors that contribute to muscle fibers’ heterogeneity is of paramount importance. Recent advances in sequencing and omics technologies have enabled comprehensive cross-verification of research on the factors affecting the types of muscle fiber across multiple levels, including the genome, transcriptome, proteome, and metabolome. These advancements have facilitated deeper exploration into the related biological questions. This review focused on the impact of individual characteristics, feeding patterns, and genetic regulation on the proportion and interconversion of different muscle fibers. The findings indicated that individual characteristics and feeding patterns significantly influence the type of muscle fiber, which can effectively enhance the type and distribution of muscle fibers in livestock. Furthermore, non-coding RNA, genes and signaling pathways between complicated regulatory mechanisms and interactions have a certain degree of impact on muscle fibers’ heterogeneity. This, in turn, changes muscle fiber profile in living animals through genetic selection or environmental factors, and has the potential to modulate the quality of fresh meat. Collectively, we briefly reviewed the structure of skeletal muscle tissue and then attempted to review the inevitable connection between the quality of fresh meat and the type of muscle fiber, with particular attention to potential events involved in regulating muscle fibers’ heterogeneity.

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Possibility of uncoupling protein 1 expression in bovine fast-twitch muscle fibers

Cited by 3 publications

References 21 publications

A possibility of uncoupling protein 1 induction with the enhancement of myogenesis related to ruminal fermentation

A possibility of uncoupling protein 1 induction with the enhancement of myogenesis related to ruminal fermentation

Differential Expression of Skeletal Muscle Sites and Fast and Slow Muscle Fibers in Mongolian Horses

Research Progress on the Regulating Factors of Muscle Fiber Heterogeneity in Livestock: A Review

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