Possible antigenic sub-divisions within the variola/vaccinia subgroup of poxviruses

Baxby, Derrick

doi:10.1007/bf01314386

Cited by 16 publications

(22 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It produced typical localized pox lesions and the virus was reisolated from these lesions. A similar observation was reported by Baxby (1972) using the CM-02 camelpox virus isolated in Iran.…”

Section: Discussionsupporting

confidence: 67%

“…The pathogenicity of these viruses to man is not well known. It is possible that these viruses may establish themselves in a less immune human population with subsequent re-evolution to cause smallpox or smallpox-like diseases (Baxby, 1975). Only minor differences in the antigenic structure of camelpox virus and smallpox virus were reported by Gispen & Brand-Saathof (1974).…”

Section: Discussionmentioning

confidence: 99%

“…Some of the isolated camelpox viruses were found to be similar to those of variola strains isolated from East Africa (Bedson, Dumbell & Thomas, 1963;Baxby, 1972). The camelpox virus was identified and classified as a virus of separate identity in the Orthopoxvirus group of the Family Poxviridae (Mahnel & Bartenbach, 1973;Mahnel, 1974;Baxby, 1975).…”

mentioning

confidence: 98%

“…The virus was identified and characterized using the conventional methods described by Baxby (1972), Marennikova et al (1974) and Davies et al (1975). The biological criteria used for virus characterization were the following: the pock morphology on the CAM, the ceiling temperature, the histopathology of the infected CAM, the lethality to chicken embryo, the growth in culture of chick embryo fibroblasts (CEF), the plaque morphology in CEF cells, the haemagglutinating activity using RBCs of human type 0, chicken and other animal species and the pathogenicity to laboratory animals (rabbit, guinea-pig, mice, 3-day-old chick and monkey).…”

Section: Virus Isolation and Characterizationmentioning

confidence: 99%

“…The camelpox virus was identified and classified as a virus of separate identity in the Orthopoxvirus group of the Family Poxviridae (Mahnel & Bartenbach, 1973;Mahnel, 1974;Baxby, 1975). Other outbreaks of 'camelpox' have been caused by vaccinia virus and a virus resembling Orf (Krupenko, 1972;Roslyakov, 1972).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Isolation, identification and characterization of camelpox virus in Iraq

Falluji

Tantawi

Shony

1979

J. Hyg.

View full text Add to dashboard Cite

summaryA virus was isolated from pox-like lesions in camels during an outbreak of camelpox disease which occurred in December 1977 in an area near the Iraqi–Iranian border. It was identified serologically as a virus of the Orthopoxvirus group. The biological properties of the isolate indicated that it was probably identical with strains of camelpox virus isolated from Iran, Egypt, Kenya and the U.S.S.R.

show abstract

Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 98%

Section: Virus Isolation and Characterizationmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Isolation, identification and characterization of camelpox virus in Iraq

Falluji

Tantawi

Shony

1979

J. Hyg.

View full text Add to dashboard Cite

show abstract

Serological relatedness of monkeypox, variola, and vaccinia viruses

Esposito

Obijeski

Nakano

1977

Journal of Medical Virology

View full text Add to dashboard Cite

Closely related human and monkey orthopoxviruses were differentiated by serologic techniques. Antiviral sera were tested by immunodiffusion for reactivity against six different viral antigens prepared from either infected cell cultures or infected chorioallantoic membranes (CAMs) of embryonated eggs. Portions of each antiserum were separately absorbed with heterologous antigens from infected CAMs to remove common reactivity. The absorbed sera formed immunodiffusion precipitates with both types of antigen preparation and revealed specific-character differences that made it possible to classify the viruses as variola, vaccinia, or monkeypox. Cross-complement fixation tests were also used to examine the immunologic reactivities of antisera to detergent-treated, purified preparations of three orthopoxviruses. Only common reactivities were detected by this method, however, and differentiating reactivities were not observed.

show abstract

Microarray assay for detection and discrimination of Orthopoxvirus species

Ryabinin

Shundrin

Kostina

et al. 2006

Journal of Medical Virology

View full text Add to dashboard Cite

A microarray method was developed for simultaneous detection and identification of six species of Orthopoxvirus (OPV) including Variola, Monkeypox, Cowpox, Camelpox, Vaccinia, and Ectromelia viruses. The method allowed us to discriminate OPV species from varicella-zoster virus (VZV), Herpes Simplex 1 virus (HSV-1), and Herpes Simplex 2 virus (HSV-2) that cause infections with clinical manifestations similar to OPV infections. The nucleotide sequences of the C23L/B29R and the B19R genes identified for 86 and 72 different OPV strains, respectively, were used to design species-specific microarray oligonucleotide probes (oligoprobes). The microarray also contained several oligoprobes selected from the ORF31, US4, and US5 genes of VZV, HSV-1, and HSV-2, respectively. The samples (from HSVs or OPVs) of ssDNAs for analyses were prepared by using asymmetric PCR followed by chemical labeling of ssDNA with Cy3 dye. DNA from 52 samples of various OPV species, two isolates of VZV, two of HSV-1, and three of HSV-2 were tested using the developed microarray assay; all tested viruses were accurately identified. To ensure the robustness of the microarray assay, three additional unrelated variola virus strains with unknown sequences of the C23L/B29R and the B19R genes were tested. In each instance the microarray unambiguously identified them as Variola virus species. The results obtained in this study demonstrated that this new microarray method is a valuable tool for the rapid and accurate detection and differentiation of these important viral pathogens.

show abstract

Possible antigenic sub-divisions within the variola/vaccinia subgroup of poxviruses

Abstract: SummaryThe members of the variola/vaccinia subgroup of poxviruses have been allocated to three sub-divisions based on the effect on them of antiserum to heated vaccinia virus.

Cited by 16 publications

References 7 publications

Isolation, identification and characterization of camelpox virus in Iraq

Isolation, identification and characterization of camelpox virus in Iraq

Serological relatedness of monkeypox, variola, and vaccinia viruses

Microarray assay for detection and discrimination of Orthopoxvirus species

Contact Info

Product

Resources

About