Possible correlations of polypeptides and Balbiani rings in the salivary glands of Drosophila auraria Peng.

Abstract

Salivary glands of various stocks of Drosophila auraria and some of its close relatives were examined with a variety of electrophoretic techniques both from larval and prepupal stages, and after ecdysterone treatments, in an effort to detect possible translation products of the two Balbiani rings (BR1 and BR2) found in the salivary gland chromosomes of these species. Two polypeptides (P2 and P1), with molecular weights of 12,000 and 53,000, respectively, have been detected, the appearance of which coincides with the presence of BR2. The results do not allow the correlation of BR1 action with any specific polypeptide(s).

An ultrastructural and histochemical developmental study of Drosophila auraria salivary gland cells during the third-instar period

“…We suspect that the number of secretory proteins is related to the structure of the secretory granules: the larger the number of secretory proteins, the more complex the structure. This is probably the case in the salivary gland cells of D. auraria as well: the secretory granules are simple, homogeneous, and filled with a granular material; only two main secretory polypeptides have been found in their secretion (Manousis and Kastritsis 1987). Two populations of secretory granules were found in each cell.…”

“…~a v r a~a n i -~s i~i d o u -and ~a s t r i t s i s 1986; Kastritsis et al 1986) and biochemical (Manousis and Kastritsis 1987) research has already been done on the salivary glands during its larval development.…”

Μορφολογική ιστοχημική και ανοσοκυτταροχημική μελέτη της επίδρασης θερμικού shock στη λεπτή δομή προνυμφικών σιαλογόνων αδένων της Drosophila auraria

“…Αρκετές μελέτες αποδεικνύουν την είσοδο των πρωτεϊνών θερμικού shock, και ιδιαίτερα των πρωτεϊνών της οικογένειας Hsp70, στον πυρηνίσκο μετά το θερμικό shock (Welch & Suhan, 1985, Ohtsuka et al, 1986, Morcillo et al, 1997 (Beermann, 1952), όπου αντιπροσωπεύουν γονίδια που κωδικοποιούν εκκριτικές πρωτεΐνες μεταξιού των προνυμφικών σιαλογόνων αδένων (Beermann, 1961, Rydlander & Edström, 1980. Στη D. auraria δεν επαληθεύεται εάν τα πολυπεπτίδια για τα οποία είναι υπεύθυνοι οι δακτύλιοι Balbiani, βρίσκονται στο τελικό εκκριτικό προϊόν κόλλας των εκκριτικών κοκκίων (Manousis & Kastritsis, 1987).…”