Possible Disease-Link Genetic Pathways Constructed by Hierarchical Clustering and Conditional Probabilities of Ovarian Carcinoma Microarray Data

Ko, Hsiao-Han; Tseng, Kuo-Jin; Wei, Limin; Tsai, Meng‐Hsiun

doi:10.1109/iihmsp.2010.8

Cited by 1 publication

(1 citation statement)

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“…ADCY6 encodes adenylyl cyclase 6, which is a membrane-associated enzyme catalyzing the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). The differential expression of this gene has also been reported in breast cancer [ 20 ] and ovarian carcinoma by microarray analysis [ 21 ]. By microarray analysis, ADCY6 expression was found to be decreased by 2-fold more in the 81-fold gemcitabine-resistant variant MiaPaCa2-RG than in the gemcitabine-sensitive cell line MiaPaCa2 [ 22 ], suggesting that high ADCY6 expression is associated with gemcitabine sensitivity.…”

Section: Discussionmentioning

confidence: 94%

RNA Sequencing Uncovers Molecular Mechanisms Underlying Pathological Complete Response to Chemotherapy in Patients with Operable Breast Cancer

Liu

Tang

et al. 2017

Med Sci Monit

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BackgroundThis study aimed to identify key genes contributing to pathological complete response (pCR) to chemotherapy by mRNA sequencing (RNA-seq).Material/MethodsRNA was extracted from the frozen biopsy tissue of patients with pathological complete response and patients with non-pathological complete response. Sequencing was performed on the HiSeq2000 platform. Differentially expressed genes (DEGs) were identified between the pCR group and non-pCR (NpCR) group. Pathway enrichment analysis of DEGs was performed. A protein-protein interaction network was constructed, then module analysis was performed to identify a subnetwork. Finally, transcription factors were predicted.ResultsA total of 673 DEGs were identified, including 419 upregulated ones and 254 downregulated ones. The PPI network constructed consisted of 276 proteins forming 471 PPI pairs, and a subnetwork containing 18 protein nodes was obtained. Pathway enrichment analysis revealed that PLCB4 and ADCY6 were enriched in pathways renin secretion, gastric acid secretion, gap junction, inflammatory mediator regulation of TRP channels, retrograde endocannabinoid signaling, melanogenesis, cGMP-PKG signaling pathway, calcium signaling pathway, chemokine signaling pathway, cAMP signaling pathway, and rap1 signaling pathway. CNR1 was enriched in the neuroactive ligand-receptor interaction pathway, retrograde endocannabinoid signaling pathway, and rap1 signaling pathway. The transcription factor-gene network consists of 15 transcription factors and 16 targeted genes, of which 5 were downregulated and 10 were upregulated.ConclusionsWe found key genes that may contribute to pCR to chemotherapy, such as PLCB4, ADCY6, and CNR1, as well as some transcription factors.

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Section: Discussionmentioning

confidence: 94%