Possible Effects of Vitamin D3 and Levofloxacin on Selected Hematology Parameter of Rats

Abstract

Vitamin D is a fat-soluble vitamin with antioxidant and DNA protecting properties , Levofloxacin is a member of the fluoroquinolone drug class, Its broad-spectrum bactericidal effect affects both Gram-positive and Gram-negative bacteria.
 The goal of the study is to analyze the haematology analysis in rats received levofloxacin and show the preventive impact of vitamin D3 by analyzing the haematology parameters: packed cell volume (PCV), mean corpuscular hemoglobin concentration(MCHC),haemoglobin (HB), red blood cell (RBC), mean corpuscular volume (MCV),meancorpuscular haemoglobin(MCH), WBC ,differential WBC, and Platelets.
 The study included 42 rats divided into 6 groups each group 7 rats. group I negative control : healthy animals received normal saline 0.9%, group II: received levofloxacin LFX dose of 50mg/kg/day (IP) for fourteen days, group III : received LFX 100mg/kg/day (IP) for fourteen days, group IV : received vitamin D3 500 IU/day orally by oral gavage for twenty one days, group V received vitamin D3 500 IU/day orally for twenty one days and levofloxacin 50 mg/kg/day IP injected at day 8 for fourteen days, group VI received vitaminD3 500IU/day for twenty one days orally and levofloxacin 100 mg/kg/day IP injected at day 8 for fourteen days.
 Blood samples taken from rats treated with levofloxacin, showed a decrease in the values of RBC, HB, PCV, MCH, MCHC, as well as a decrease in total white blood cells, then returned approximately to normal levels in the groups IV ,group V and group VI .
 from the result of the study conclude that some haematological changes caused by levofloxacin ameliorated by vitamin D3 may be due to indirect effect of vitamin D3 on haematology parameters.

Study the Relationship between polymorphisms of Vitamin D Receptor (VDR FokI and VDR ApaI) Genes and colorectal cancer in some Iraqi population

“…It was not statistically significant for either the genotype distribution of ApaI or the polymorphism of FokI. Compared to the healthy group and benign cases, whereas statistically significant variation was discovered in the median VD blood levels when compared serum levels between benign and cancer cases [48,49,50] Polymorphisms in the VD receptor gene vary by ethnicity. Because the examined SNPs had no significant association in our group, their link with other functionally recognized gene polymorphisms may play an important role in CRC.…”