Possible Errors in the Analysis of Lactic Acid and Volatile Fatty Acids in the Gastrointestinal Tracts of Pigs and Chickens

Clayton, Edward; Blake, Robert R.

doi:10.1128/aem.71.4.2206-2207.2005

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…phenolic absorbance at 630 nm was measured using a spectrophotometer [20]. Gas chromatography (GC-2010, Shimadzu, Japan) was used to measure the VFA concentration in the sheep rumen fluid [21]. And 4-methyl valaric was used as internal standard.…”

Section: Rumen Nh 3 -N and Vfas Measurementmentioning

confidence: 99%

Effect of dietary gossypol supplement on fermentation characteristics and bacterial diversity in the rumen of sheep

Wang

Maitisaiyidi

et al. 2020

PLoS ONE

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The tolerance of ruminants to gossypol, a natural phenolic compound derived from the cotton plant, is greater than that of monogastric animals, partially because of the gossypoldegrading bacteria in the rumen of the ruminants. In this study, we aimed to examine the effect of gossypol supplementation on fermentation characteristics, bacterial α-diversity and community structure in the rumen fluid of sheep to analyse the change of bacterial in response to gossypol. 8 sheep with permanent fistula were randomly divided into 2 groups, a control and gossypol acetate supplementation groups. Sheep in the latter group were supplemented with gossypol acetate at the levels of 600 mg and 1,200 mg/animal per day during the first (S1, days 1 to 27) and subsequent (S2, days 28 to 47) stages. Gossypol supplementation significantly increased the molar proportion of acetate, and decreased the molar proportion of isobutyric acid, butyric acid, and isovaleric acid in the rumen fluid. Gossypol supplementation have no significant effect on bacterial diversity in the rumen fluid. At the phylum level, gossypol had no effect on bacterial community. At the genus level, gossypol supplementation significantly increased the relative abundance of Treponema_2. However, there were no significant differences in the relative abundance of dominant bacterial genera. In conclusion, gossypol supplementation had an effect on molar proportion of acetate, isobutyric acid, butyric acid, and isovaleric acid, but had no significant effect on the bacterial diversity and relative abundance of dominant bacteria in rumen fluid of sheep.

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Section: Rumen Nh 3 -N and Vfas Measurementmentioning

confidence: 99%