Possible involvement of abscisic acid in the induction of secondary somatic embryogenesis on seed-coat-derived carrot somatic embryos

Ogata, Yumiko; Iizuka, Misato; Nakayama, Daisuke; Ikeda, Miho; Kamada, Hiroshi; Koshiba, Tomokazu

doi:10.1007/s00425-004-1449-5

Cited by 24 publications

(20 citation statements)

References 29 publications

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“…In current study secondary embryos were identified in maturation medium. Some studies have correlated the formation of secondary somatic embryos depending on the concentration of exogenous ABA (Iida et al 1992, Ogata et al 2005, Aman and Afrasiab 2014 or the types of somatic embryos placed in MM with ABA (Lee et al 1998, Calic et al 2012.…”

Section: Maturation Of Somatic Embryosmentioning

confidence: 99%

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

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The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heartshape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L -1 ) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus -1 ) and the lowest percentage of early germination (4%).

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Section: Maturation Of Somatic Embryosmentioning

confidence: 99%

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

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“…9,11,13 and in dicots (Daucus carota, Hevea). 8,10,12 Our experiment in chicory established that 50 mM ABA promoted the ISE whereas 100 mM had a slightly negative effect. Thus, our results are in agreement with results already described in the literature.…”

Section: Discussionmentioning

confidence: 72%

“…[5][6][7] In various models of SE, the exogenous supply of abscisic acid (ABA), a well-known stress hormone of plants, also led to increase the ISE. [8][9][10][11][12] Also, the report of an extracellular matrix connecting cell wall-plasmalemma-cytoskeleton led to the hypothesis that the cell wall could be sensitive to osmotic stresses. [13][14][15][16] Supporting this possibility, several groups reported that treatments with plasmolysing agents led to increase the ISE of gymnosperms and Eleutherococcus.…”

Section: Introductionmentioning

confidence: 99%

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Couillerot

Windels

Vazquez

et al. 2012

Plant Signaling & Behavior

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Somatic embryogenesis (SE) in Cichorium involves dedifferentiation and redifferentiation of single cells and can be induced by specific in vitro culture conditions. We have tested the effect of various treatments on the incidence of SE (ISE) of an interspecific embryogenic hybrid (C. endivia x C. intybus) and of different commercial chicories (C. endivia and C. intybus) that are typically recalcitrant to SE in standard culture conditions. We found that the ISE of the hybrid is significantly increased by pretreatment of tissues by submersion in solutions of glycerol, abscisic acid, spermine, putrescine or of combinations of these compounds. Interestingly, the most efficient of these pretreatments also had an unexpectedly high effect on the ISE of the C. intybus cultivars. The ISE of the hybrid and of the commercial chicories were increased when explants were co-cultured with highly embryogenic chicory explants or when they were cultured in conditioned medium. These observations established that unidentified SE-promoting factors are released in the culture medium. HPLC analyses of secreted Arabino-Galactan Proteins (AGPs), which are known to stimulate SE, did not allow identifying a fraction containing differentially abundant AGP candidates. However, pointing to their role in promoting SE, we found that the hybrid had a drastically higher ISE when amino sugars and L-Proline, the putative precursors of secreted AGPs, were both added to the medium.

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“…The most effective method seems to be somatic embryogenesis (SE) (Gude and Dijkema 1997), which enables large-scale propagation of many geophytes (Stefaniak 1994; Nhut et al 2006;Bakhshaie et al 2010;Gao et al 2010;Subotić et al 2010). Somatic embryogenesis also makes it possible to investigate factors involved in the process (Find 1997;Senger et al 2001) and to study the physiological, biological, and molecular events occurring during embryo development (Zimmerman 1993;Ogata et al 2005;Quiroz-Figueroa et al 2006). To induce somatic organogenesis, the culture medium is supplemented mainly with an auxin, which determines the initiation of somatic embryos (Zimmerman 1993;Dodeman et al 1997;Gude and Dijkema 1997;Raghavan 1997, Bach andPtak 2001).…”

Section: Introductionmentioning

confidence: 99%

“…BAP (Malik 2008;Maślanka and Bach 2010). Initiated somatic embryos can develop into whole plants under appropriate conditions (Ogata et al 2005). For proper maturation and conversion, somatic embryos must first achieve morphological maturity and then-by undergoing a desiccation period-physiological maturity (Stasolla and Yeung 2003).…”

Section: Introductionmentioning

confidence: 99%

Endogenous ABA content in relation to maturation of somatic embryos in Tulipa (L.) ‘Apeldoorn’ cultures

2016

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The aim of the present study was to estimate the endogenous abscisic acid (ABA) content in tulip 'Apel-doorn' torpedo and mature somatic embryos. Moreover, the effect of exogenous ABA and/or its inhibitor fluridone on somatic embryo maturation and conversion into plantlets was investigated. Torpedo-stage somatic embryos were subcultured on media containing 5 lM of picloram and 1 lM of 6-benzyl-aminopurine (BAP)-control, and combinations of ABA (0 or 10 lM) and/or fluridone (0 or 30 lM) for 1 week. Then, the torpedo embryos were transferred to a maturation medium containing 0.25 lM of a-naphthaleneacetic acid (NAA) and 2.5 lM of BAP, without ABA and fluridone treatment, and cultivated under darkness or light for ten weeks. Endogenous ABA content (first time measured in tulip somatic embryos) was evaluated by ELISA test. The obtained results revealed that the highest level of endogenous ABA, at 17.45 nmol g-1 dry weight (DW), was recorded in torpedo-stage of tulip embryo development, only after 1 week of ABA treatment, and was nearly 10 times higher in comparison with the control. Simultaneous addition of ABA and fluridone to the medium resulted in the lowering of the ABA concentration to 9.58 nmol g-1 DW. During ten weeks of maturation of the embryos, the endogenous ABA content in mature tissue of tulip somatic embryo considerably decreased to an amount 0.87-1.33 nmol g-1 DW (irrespective of ABA and fluridone treatment) and did not differ significantly from control (0.59 nmol g-1 DW). Exogenous ABA and fluridone significantly decreased the growth value of fresh weight (FW) of the tulip torpedo-shaped and mature embryos under light conditions. Percentage of the DW of the torpedo embryos treated with exogenous ABA was significantly higher (15.43-17.02) in comparison with the control (10.87). Three to three and a half times more malformed mature embryos were noted under light conditions than in darkness, irrespective of ABA and fluridone treatment. The highest percentage of mature embryos forming shoots (conversion) was observed under light conditions in the control and after fluridone treatment (26 and 20%, respectively).

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Possible involvement of abscisic acid in the induction of secondary somatic embryogenesis on seed-coat-derived carrot somatic embryos

Cited by 24 publications

References 29 publications

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Endogenous ABA content in relation to maturation of somatic embryos in Tulipa (L.) ‘Apeldoorn’ cultures

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