Possible Involvement of Insulin-like Growth Factor-I in Mediating the Stimulatory Effect of Recombinant Bovine Growth Hormone on Maturation of Bovine Oocytes In Vitro.

Iga, Kosuke; Niwa, K.; Bartke, Andrzej

doi:10.1262/jrd.44.243

Cited by 5 publications

(9 citation statements)

References 36 publications

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“…When bovine COCs were cultured in the presence of GH and anti-IGF-I antibodies, conflicting results were obtained. Iga et al showed that the stimulatory effects of GH are mediated via IGF-I [29], whereas Izadyar et al concluded that GH-induced oocyte maturation is not mediated by IGF-I [12], but is mediated by the cAMP signal transduction pathway [30]. In order to elucidate the mechanism of GH-induced nuclear maturation in equine and porcine oocytes, we first investigated whether equine and porcine COCs contain GH receptor.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Growth Hormone (GH) on In Vitro Nuclear and Cytoplasmic Oocyte Maturation, Cumulus Expansion, Hyaluronan Synthases, and Connexins 32 and 43 Expression, and GH Receptor Messenger RNA Expression in Equine and Porcine Species1

Marchal

Caillaud

Martoriati

et al. 2003

Biology of Reproduction

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The aim of this study was to investigate the role of growth hormone (GH) on in vitro cumulus expansion and oocyte maturation in equine and porcine cumulus-oocyte complexes (COCs), and to approach its way of action. Equine COCs were cultured in a control medium (TCM199, 5 mg/ml BSA, 1 g/ml estradiol, and antibiotics) supplemented with either 0.5 g/ml equine GH or 5 g/ml equine LH. Porcine COCs were cultured in a basal medium (TCM199 with 570 M cysteamine) supplemented with 0, 0.1, 0.5, or 1 g/ml porcine GH or in a control medium (basal medium with 10 ng/ml epidermal growth factor and 400 ng/ml FSH) supplemented with 0 or 0.5 g/ml porcine GH. After culture, cumulus expansion and nuclear stage were assessed. The cytoplasmic maturation of porcine oocytes was evaluated by in vitro fertilization and development for 7 days. The modifications of the expression of proteins implicated in cumulus expansion were analyzed in equine COCs by SDS-PAGE with antibodies against connexins 32 and 43 and hyaluronan synthases (Has) 1, 2, and 3. The expression of GH receptor mRNA was studied in oocytes and cumulus cells of the two species using reverse transcription-polymerase chain reaction with specific primers. The addition of GH in maturation medium increased cumulus expansion in equine but not porcine COCs. It improved nuclear maturation in equine and porcine, but had no effect on porcine fertilization and embryo development. The GH receptor mRNA was detected in equine and porcine oocytes and cumulus cells. GH did not influence the expression of Has 1, Has 3, and connexin 43 in equine cumulus cells. cumulus cells, gamete biology, growth hormone, oocyte development, ovum INTRODUCTIONGonadotropins are the major regulators of ovarian function. In the last few years, however, there has been evidence that growth factors and metabolic hormones are also involved in ovarian regulation. The role of growth hormone (GH) in ovarian functions, follicular growth, and steroidogenesis is well known [1, 2 for review], and some evidence shows a positive effect of GH on oocyte maturation.

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Section: Discussionmentioning

confidence: 99%

“…In the rat, GH effects are mediated by IGF-I [21]. In bovine, some studies showed that GH acts via IGF-I [29], while others showed that GH acts via a cAMP-mediated pathway without mediation of IGF-I [12,30]. To our knowledge, no studies have been published in other species.…”

Section: Introductionmentioning

confidence: 96%

Effect of Growth Hormone (GH) on In Vitro Nuclear and Cytoplasmic Oocyte Maturation, Cumulus Expansion, Hyaluronan Synthases, and Connexins 32 and 43 Expression, and GH Receptor Messenger RNA Expression in Equine and Porcine Species1

Marchal

Caillaud

Martoriati

et al. 2003

Biology of Reproduction

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“…GH appears to play a relevant role in regulating oocyte growth and maturation as a positive relationship between GH concentrations in the follicular fluid and oocyte maturation has been observed (Mendoza et al, 1999; Bevers and Izadyar, 2002). This activity is reportedly mediated by insulin‐like growth factor 1 (IGF‐I; Iga et al, 1998), although other authors (Izadyar et al, 1997a, b) have suggested that GH activates the cAMP pathway without the involvement of IGF‐I.…”

Section: Discussionmentioning

confidence: 99%

[Ca²⁺]_i rise at in vitro maturation in bovine cumulus–oocyte complexes

Silvestre

Fissore

Tosti

et al. 2012

Molecular Reproduction Devel

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An intracellular calcium ([Ca(2+)]i) rise has been described in cumulus-oocyte complexes (COCs) following luteinizing hormone (LH) exposure. Together with cAMP, Ca(2+) is a candidate signal for resumption of meiosis. Here, we analyzed if the most common hormones involved in oocyte maturation can induce the same Ca(2+) signal. In addition, we characterized the source of this signal. Immature, in vitro-matured, and roscovitine-meiotically arrested COCs were loaded with Fluo-4 AM, stimulated with hormones/growth factors, and tested for [Ca(2+)](i) variations in cumulus cells. Reagents known to inhibit or stimulate [Ca(2+)](i) rises were used to characterize these [Ca(2+)](i) dynamics. Finally, expression of LH receptors (LHRs) in COCs was analyzed by immunofluorescence. In immature COCs, follicle-stimulating hormone (FSH) elicited a single [Ca(2+)](i) rise that was higher than those induced by LH and growth hormone (GH), whereas epithelial growth factor failed to induce any changes in [Ca(2+)](i). The [Ca(2+)](i) rise induced by FSH was higher in immature COCs; was reduced in roscovitine-arrested, immature COCs; and was negligible in gonadotropin-induced, in vitro-matured COCs. In the case of spontaneous- and GH-matured COCs, however, FSH stimulation caused a lower [Ca(2+)](i) rise. The hormone-induced [Ca(2+)](i) rise was due to: (i) external Ca(2+) entry; (ii) intercellular communication; and (iii) intracellular Ca(2+) stores. Immunofluorescence revealed that LHRs were expressed throughout the cumulus cells. The above results show that: (i) gonadotropins and GH cause a [Ca(2+)](i) rise in cumulus cells; (ii) this [Ca(2+)](i) rise results from extra-, inter-, and intra-cellular cumulative Ca(2+) fluxes; and (iii) LHRs are distributed on either outer or inner cumulus cells.

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“…1997), accelerates nuclear maturation (Izadyar et al. 1996a; Iga et al. 1998a,b) and increases maturation rate, followed by embryonic development until blastocyst stage (Iga et al.…”

Section: Introductionmentioning

confidence: 99%

“…1996a; Iga et al. 1998a,b) and increases maturation rate, followed by embryonic development until blastocyst stage (Iga et al. 1998a,b).…”

Section: Introductionmentioning

confidence: 99%

Addition of Recombinant Human Growth Hormone to In Vitro Maturation Medium of Bovine Oocytes

Pozzobon¹,

Lagares²,

Brum³

et al. 2005

Reprod Domestic Animals

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The aim of this study was to increase the bovine embryonic development rate, adding recombinant human growth hormone (rhGH) to maturation medium of bovine oocytes. Oocytes were matured for 24 h in TCM 199 Earle's salts and five treatments were developed: T1, 0.01 IU/ml of recombinant human follicle stimulating hormone (rhFSH); T2, 0.01 IU/ml of rhFSH + 100 ng/ml of rhGH; T3, 0.01 IU/ml of rhFSH + 1000 ng/ml of rhGH; T4, 100 ng/ml of rhGH; and T5, 1000 ng/ml of rhGH at 39 degrees C and 5% of CO(2) in air and saturated humidity. In vitro fertilization from cumulus-oocyte complexes was conducted in TALP-Fert medium (18-22 h) and spermatozoa were selected by Percoll gradient. Zygotes were incubated in SOFaaci medium in 5% of CO(2) in air, 5% of O(2) at 39 degrees C and saturated humidity for 11 days. There was no statistical difference in cleavage rate and embryo production on day 7 and day 9 among treatments. However, the hatching rate increased significantly in the T4 and T5 treatments (11.0 and 12.8%, respectively), compared with the T1 treatment (4.6%) (p < 0.05). Therefore, the rhGH addition to the oocyte maturation medium showed beneficial effects on the hatching rate of in vitro-produced bovine embryos.

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Possible Involvement of Insulin-like Growth Factor-I in Mediating the Stimulatory Effect of Recombinant Bovine Growth Hormone on Maturation of Bovine Oocytes In Vitro.

Cited by 5 publications

References 36 publications

Effect of Growth Hormone (GH) on In Vitro Nuclear and Cytoplasmic Oocyte Maturation, Cumulus Expansion, Hyaluronan Synthases, and Connexins 32 and 43 Expression, and GH Receptor Messenger RNA Expression in Equine and Porcine Species1

Effect of Growth Hormone (GH) on In Vitro Nuclear and Cytoplasmic Oocyte Maturation, Cumulus Expansion, Hyaluronan Synthases, and Connexins 32 and 43 Expression, and GH Receptor Messenger RNA Expression in Equine and Porcine Species1

[Ca²⁺]_i rise at in vitro maturation in bovine cumulus–oocyte complexes

Addition of Recombinant Human Growth Hormone to In Vitro Maturation Medium of Bovine Oocytes

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Possible Involvement of Insulin-like Growth Factor-I in Mediating the Stimulatory Effect of Recombinant Bovine Growth Hormone on Maturation of Bovine Oocytes In Vitro.

Cited by 5 publications

References 36 publications

Effect of Growth Hormone (GH) on In Vitro Nuclear and Cytoplasmic Oocyte Maturation, Cumulus Expansion, Hyaluronan Synthases, and Connexins 32 and 43 Expression, and GH Receptor Messenger RNA Expression in Equine and Porcine Species1

Effect of Growth Hormone (GH) on In Vitro Nuclear and Cytoplasmic Oocyte Maturation, Cumulus Expansion, Hyaluronan Synthases, and Connexins 32 and 43 Expression, and GH Receptor Messenger RNA Expression in Equine and Porcine Species1

[Ca2+]i rise at in vitro maturation in bovine cumulus–oocyte complexes

Addition of Recombinant Human Growth Hormone to In Vitro Maturation Medium of Bovine Oocytes

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[Ca²⁺]_i rise at in vitro maturation in bovine cumulus–oocyte complexes