Possible involvement of &lt;i&gt;Pseudomonas fluorescens&lt;/i&gt; and Bacillaceae in structural modifications of &lt;i&gt;Tuber borchii&lt;/i&gt; fruit bodies

Citterio, Barbara; Malatesta, Manuela; Battistelli, Serafina; Marcheggiani, F.; Baffone, Wally; Saltarelli, Roberta; Stocchi, Vilberto; Gazzanelli, Giancarlo

doi:10.1139/cjm-47-3-264

Cited by 27 publications

(19 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More comprehensive studies on characterizing the bacterial communities using next generation sequencing methods have focused of truffles and similar ascomycetes with belowground fruitbodies (e.g., Antony-Babu et al, 2014; Benucci and Bonito, 2016). Bacteria may have several symbiotic functions in mushrooms, such as inhibiting pathogens and antagonists (Tsukamoto et al, 2002; Frey-Klett et al, 2007), improving the distribution of spores (Citterio et al, 2001; Splivallo et al, 2014) or providing vitamins and growth regulators (Rangel-Castro et al, 2002a; Riedlinger et al, 2006). Several fungal-associated bacteria are also known to fix nitrogen (Jayasinghearachchi and Seneviratne, 2004; Paul et al, 2007; Barbieri et al, 2010; Hoppe et al, 2014), although there is yet no evidence that the fungus directly benefits from that ability of the associated bacteria.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Communities in Boreal Forest Mushrooms Are Shaped Both by Soil Parameters and Host Identity

2017

View full text Add to dashboard Cite

Despite recent advances in understanding the microbiome of eukaryotes, little is known about microbial communities in fungi. Here we investigate the structure of bacterial communities in mushrooms, including common edible ones, with respect to biotic and abiotic factors in the boreal forest. Using a combination of culture-based and Illumina high-throughput sequencing, we characterized the bacterial communities in fruitbodies of fungi from eight genera spanning four orders of the class Agaricomycetes (Basidiomycota). Our results revealed that soil pH followed by fungal identity are the main determinants of the structure of bacterial communities in mushrooms. While almost half of fruitbody bacteria were also detected from soil, the abundance of several bacterial taxa differed considerably between the two environments. The effect of host identity was significant at the fungal genus and order level and could to some extent be ascribed to the distinct bacterial community of the chanterelle, representing Cantharellales—the earliest diverged group of mushroom-forming basidiomycetes. These data suggest that besides the substantial contribution of soil as a major taxa source of bacterial communities in mushrooms, the structure of these communities is also affected by the identity of the host. Thus, bacteria inhabiting fungal fruitbodies may be non-randomly selected from environment based on their symbiotic functions and/or habitat requirements.

show abstract

Section: Introductionmentioning

confidence: 99%

Bacterial Communities in Boreal Forest Mushrooms Are Shaped Both by Soil Parameters and Host Identity

2017

View full text Add to dashboard Cite

show abstract

“…These interact with the truffle mycelium in four kinds of environments: soil hyphae, ectomycorrhizae, ascocarps and stromata. Most currently available information was obtained from ascocarps (Bedini et al ., ; Citterio et al ., ) and ectomycorrhizae (Sbrana et al ., ). The data from soil are rather rare (Napoli et al ., ; Mello et al ., ) and information on microbes associated with truffle stromata (Pargney and Jalade, ) is still missing.…”

Section: Introductionmentioning

confidence: 99%

A quest for indigenous truffle helper prokaryotes

Gryndler

Soukupová

Hršelová

et al. 2012

Environ Microbiol Rep

View full text Add to dashboard Cite

Tuber aestivum is the most common European truffle with significant commercial exploitation. Its production originates from natural habitats and from artificially inoculated host tree plantations. Formation of Tuber ectomycorrhizae in host seedling roots is often inefficient. One possible reason is the lack of indigenous associative microbes. Here we aimed at metagenetic characterization and cultivation of indigenous prokaryotes associated with T. aestivum in a field transect cutting through the fungus colony margin. Several operational taxonomic units (OTUs) showed close association with the T. aestivum in the ectomycorrhizae and in the soil, but there was no overlap between the associative prokaryotes in the two different habitats. Among those positively associated with the ectomycorrhizae, we identified several bacterial genera belonging to Pseudonocardineae. Extensive isolation efforts yielded many cultures of ectomycorrhizae-associative bacteria belonging to Rhizobiales and Streptomycineae, but none belonging to the Pseudonocardineae. The specific unculturable Tuber-associated prokaryotes are likely to play important roles in the biology of these ectomycorrhizal fungi, including modulation of competition with other symbiotic and saprotrophic microbes, facilitation of root penetration and/or accessing mineral nutrients in the soil. However, the ultimate proof of this hypothesis will require isolation of the microbes for metabolic studies, using novel cultivation approaches.

show abstract

“…Bacteria belonging to Pseudomonadales, associated to Tuber borchii ascocarps, have been isolated and characterised. Some strains were shown to restrict growth of phytopathogenic fungi, to possess cellulolytic, chitinolytic and proteolytic activities in vitro and to release metabolites, both medium‐diffusible and volatiles, affecting T. borchii mycelial growth and morphogenesis in culture [21–25].…”

Section: Introductionmentioning

confidence: 99%

Diversity of culturable bacterial populations associated toTuber borchiiectomycorrhizas and their activity onT. borchiimycelial growth

Sbrana

Agnolucci

Bedini

et al. 2002

FEMS Microbiology Letters

View full text Add to dashboard Cite

Isolation and physiological and molecular characterisation of culturable bacterial strains belonging to actinomycetes, pseudomonads and aerobic spore-forming bacteria were carried out on mycorrhizal root tips of Quercus robur var. peduncolata infected by Tuber borchii. Cellular density of the three bacterial groups in ectomycorrhizal root tips was estimated to be 1.3+/-0.11 x 10(6) cfu g(-1) dry weight for total heterotrophic bacteria and 1.08+/-0.6 x 10(5) (mean+/-S.E.), 1.3+/-0.3 x 10(5) and 1.4+/-0.2 x 10(5) cfu g(-1) dry weight for pseudomonads, actinomycetes and spore-forming bacteria respectively. Identification of pseudomonads by the Biolog system indicated, besides the most represented species Pseudomonas fluorescens (biotypes B, F and G), the occurrence of strains belonging to Pseudomonas corrugata. Amplified ribosomal DNA restriction analysis of actinomycetes and spore formers revealed at least three and six different groups of patterns, respectively. Many bacterial isolates were able to induce variations in growth rates of T. borchii mycelium; among these, 101 strains showed antifungal activity, whereas 17 isolates, belonging to spore formers, were able to increase mycelial growth up to 78% when compared to uninoculated mycelial growth. The potential role of these populations in the development and establishment of mycorrhizas is discussed.

show abstract

Possible involvement of <i>Pseudomonas fluorescens</i> and Bacillaceae in structural modifications of <i>Tuber borchii</i> fruit bodies

Cited by 27 publications

References 0 publications

Bacterial Communities in Boreal Forest Mushrooms Are Shaped Both by Soil Parameters and Host Identity

Bacterial Communities in Boreal Forest Mushrooms Are Shaped Both by Soil Parameters and Host Identity

A quest for indigenous truffle helper prokaryotes

Diversity of culturable bacterial populations associated toTuber borchiiectomycorrhizas and their activity onT. borchiimycelial growth

Contact Info

Product

Resources

About