Possible involvement of macrophages in embryo–maternal relationships during ovum implantation in the rat

Tachi, Chikashi; Tachi, Sumie; Knyszynski, Ahuva; Lindner, H. R.

doi:10.1002/jez.1402170109

Cited by 39 publications

(18 citation statements)

References 12 publications

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“…Macrophages, well-documented sources of TNF, are present in the uteri of rats, humans, and mice (8,25,(35)(36)(37)(38)(39). TNF gene expression in these cells appears to differ among the three species.…”

Section: Discussionmentioning

confidence: 99%

Normal Distribution of Tumor Necrosis Factor-α Messenger Ribonucleic Acid and Protein in the Uteri, Placentas, and Embryos of Osteopetrotic (op/op) Mice Lacking Colony-Stimulating Factor-11

Hunt¹,

Chen²,

Hu³

et al. 1993

Biology of Reproduction

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In order to map mouse tumor necrosis factor-alpha (TNF) gene expression in detail and to determine whether transcription or translation of the TNF gene is regulated by uterine colony stimulating factor-1 (CSF-1), preimplantation embryos, oviducts, uteri, and uteroplacental units were studied in various strains of mice. These included homozygous osteopetrotic (op/op) female mice, which completely lack CSF-1, and heterozygous (+/op) females, which have normal levels of CSF-1. TNF mRNA was identified in all samples except preimplantation embryos by use of Northern blot hybridization or reverse transcriptase polymerase chain reaction. In situ hybridization and immunocytochemical experiments showed that the TNF gene was expressed in mouse oviduct and uterine epithelial cells, decidual cells, macrophage-like cells, placental trophoblast, and embryos. Despite an absence of CSF-1, TNF gene expression in the uteri, placentas, and embryos of op/op mothers did not differ in any major respect from expression in +/op or other strains of mice. The results of this study therefore indicate that the TNF gene is transcribed and translated in an ordered sequence through mouse gestation, and that maternal CSF-1 is not essential to expression of this cytokine gene. Collectively, these findings are consistent with a major role for TNF in mouse reproduction and development and with a potential compensatory function for this potent polypeptide factor in CSF-1 deficiency.

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Section: Discussionmentioning

confidence: 99%

Normal Distribution of Tumor Necrosis Factor-α Messenger Ribonucleic Acid and Protein in the Uteri, Placentas, and Embryos of Osteopetrotic (op/op) Mice Lacking Colony-Stimulating Factor-11

Hunt¹,

Chen²,

Hu³

et al. 1993

Biology of Reproduction

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“…Macrophages [19] and eosinophils [18] are also present, and they have functions similar to those of neutrophils [43]. Neutrophils, macrophages, and eosinophils can all degrade extracellular matrix components and increase vascular permeability.…”

Section: Discussionmentioning

confidence: 99%

“…Neutrophils are present in rat endometrium around the time of implantation [17][18][19], and in mice an increase in endometrial neutrophil numbers follows the application of Accepted January 16, 1993. Received October 23, 1992 a deciduogenic stimulus to the sensitized uterus [10,20]. The role of neutrophils within the uterus is not known.…”

Section: Introductionmentioning

confidence: 99%

An Investigation into the Role of Neutrophils in Decidualization and Early Pregnancy in the Rat1

Orlando-Mathur

Kennedy

1993

Biology of Reproduction

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Possible roles for neutrophils in the response of sensitized rat uteri to a deciduogenic stimulus were investigated. Ovariectomized rats were sensitized for the decidual cell reaction by the administration of estradiol and progesterone. Sesame oil was injected into one uterine horn as a deciduogenic stimulus on the equivalent of Day 5 of pseudopregnancy. Neutrophil numbers in the endometrium, as determined by microscopy after application of the stimulus, were dependent on time and stimulation. Very few neutrophils were found in the endometrium before stimulation. To determine whether neutrophils were involved in the uterine responses to a deciduogenic stimulus, rats were depleted of circulating neutrophils by prior treatment with either methotrexate (MET) or antineutrophil serum (ANS). The increase in endometrial vascular permeability, as quantified with [125I]-BSA 10 h after stimulation, was not significantly affected by MET or ANS treatment, nor was the extent of decidualization, as indicated by the weight of the uteri 5 days after stimulation. Endometrial neutrophil numbers were significantly lower in the MET- and ANS-treated animals compared to their respective controls. In the uterus, activity of myeloperoxidase, an enzyme found mainly in neutrophils, was significantly reduced in MET-treated animals. To investigate the role of neutrophils in blastocyst implantation, we treated rats with ANS early on Day 5 of pregnancy. On Day 6, neither the numbers of implantation sites nor their weights were affected by ANS treatment. ANS treatment on Day 5 of pregnancy did not attenuate the numbers and weights of placentae and fetuses on Days 19 and 20 of pregnancy.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…This is shown by trauma causing decidualization in the appropriate endocrine milieu [2], the possible role of histamine [3], the mac rophage response in deciduali/ation [4] and the role of prostaglandins [5]. It is known that semen contains lysozymes, prostaglandins [6,7] and excites a macrophage response [8].…”

Section: Introductionmentioning

confidence: 99%

Influence of Insemination on the Implantation of Transferred Rat Blastocysts

Carp

Serr

Mashiach

et al. 1984

Gynecol Obstet Invest

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Embryo transfer probably produces a lower incidence of implantation than the physiological incidence despite all other factors seeming similar. The only factor known to be present physiologically and absent in embryo transfer is the presence of sperm in the uterine cavity. Implantation and deciduahzation are often considered a modified form of inflammatory reaction. Semen contains factors which excite an inflammatory response. This project attempted to determine whether insemination would affect the implantation rate of transferred blastocysts in the rat. The figures showed a significantly increased implantation rate after insemination at day 4 of pseudopregnancy as compared to controls.

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Possible involvement of macrophages in embryo–maternal relationships during ovum implantation in the rat

Cited by 39 publications

References 12 publications

Normal Distribution of Tumor Necrosis Factor-α Messenger Ribonucleic Acid and Protein in the Uteri, Placentas, and Embryos of Osteopetrotic (op/op) Mice Lacking Colony-Stimulating Factor-11

Normal Distribution of Tumor Necrosis Factor-α Messenger Ribonucleic Acid and Protein in the Uteri, Placentas, and Embryos of Osteopetrotic (op/op) Mice Lacking Colony-Stimulating Factor-11

An Investigation into the Role of Neutrophils in Decidualization and Early Pregnancy in the Rat1

Influence of Insemination on the Implantation of Transferred Rat Blastocysts

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