Possible Mechanisms of Damage Which Predispose Heart Valves to Endocarditis

Cooper, M D; Jeffery-Wiseman, Christine; Bortner, Carol A.; Wheat, L. Joseph; Wadström, Torkel

doi:10.1007/978-1-4471-3454-1_48

Pathogenesis of Wound and Biomaterial-Associated Infections 1990

DOI: 10.1007/978-1-4471-3454-1_48

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Possible Mechanisms of Damage Which Predispose Heart Valves to Endocarditis

M D Cooper¹,

Christine Jeffery-Wiseman²,

Carol A. Bortner³

et al.

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1993

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A method for the enumeration of bacterial adhesion to epithelial cells using image analysis

Evans-Hurrell

Adler

Denyer

et al. 1993

FEMS Microbiology Letters

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Computerised image analysis was utilised to enumerate the attachment of Staphylococcus epidermidis to HEp2 cell monolayers. A differential staining technique was employed such that individual staphylococcal cells stood out in sharp contrast against the uneven cell surface and granular contents of the epithelial cells. The primary image analysis operation involved subtracting an out‐of‐focus image from an in‐focus image of the bacteria on the monolayer, thereby accentuating the bacterial image. Enumeration, using a particle counting routine, was rapid and reproducible, facilitating counting in excess of 700 bacteria per field at ×500 magnification. The computerised programme compared favourably with manual counting and would provide a rapid, objective and morphologically discriminatory method for evaluating bacterial attachment to various tissues.

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A method for the enumeration of bacterial adhesion to epithelial cells using image analysis

Evans-Hurrell

Adler

Denyer

et al. 1993

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

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Possible Mechanisms of Damage Which Predispose Heart Valves to Endocarditis

Cited by 1 publication

References 12 publications

A method for the enumeration of bacterial adhesion to epithelial cells using image analysis

A method for the enumeration of bacterial adhesion to epithelial cells using image analysis

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