Possible mechanisms of the antimicrobial effects of polypeptide‑enriched Gastrodia elata Blume extracts

Kong, Fange; Cai, X. Y.; Zhai, Siyu; Wang, Ruochen; Zheng, Xiaoyi; Ma, Yue; Bi, Hui; Wang, Di

doi:10.3892/mmr.2019.10706

Cited by 2 publications

(3 citation statements)

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“…Antimicrobial peptides often induce fungal morphology and structure changes ( Vieira Bard et al, 2014 ; Kong et al, 2019 ). To assess the possible mechanism of ToAP2D action, the morphology of S. globosa was observed using a scanning electron microscope (SEM).…”

Section: Resultsmentioning

confidence: 99%

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Antifungal Activity of ToAP2D Peptide Against Sporothrix globosa

Yan

et al. 2021

Front. Bioeng. Biotechnol.

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Improving clinical efficacy and reducing treatment time have been the focus of sporotrichosis therapy. Antimicrobial peptides ToAP2A, ToAP2C, and ToAP2D were synthesized on the basis of ToAP2 (AP02759), a peptide derived from the antimicrobial peptide database by the database filtering technology, and their physicochemical characteristics were analyzed. Compared with template peptide ToAP2, the modified peptides had much shorter length, lower molecular weight but significantly greater stability, which in return resulted in increases in the aliphatic index, hydrophilicity, and protein binding ability. Here, we show that the three derived peptides inhibit the growth of Sporothrix globosa, among which ToAP2D had the strongest anti-fungal activity. ToAP2D showed good serum stability without acute toxicity. The ToAP2D treatment inhibited the growth of S. globosa and enhanced apoptosis, which was evidenced by the upregulation of apoptosis-related protein caspase-3. The scanning electron microscopy analysis revealed deformation and rupture of S. globosa. The levels of mitochondrial membrane potential were decreased and that of the reactive oxygen species (ROS) were increased in S. globosa upon ToAP2D treatment. Moreover, ToAP2D activated metacaspase. In the in vivo study, we further demonstrated that ToAP2D inhibited the S. globosa infection of mice footpads, and its efficiency was nearly comparable to itraconazole. In summary, our results suggest that antimicrobial peptide ToAP2D has the potential for sporotrichosis therapy.

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Section: Resultsmentioning

confidence: 99%

“…Antimicrobial peptides often induce fungal morphology and structure changes (Vieira Bard et al, 2014;Kong et al, 2019).…”

Section: S Globosa Morphological Changes After Toap2d Treatment In Vitromentioning

confidence: 99%

Antifungal Activity of ToAP2D Peptide Against Sporothrix globosa

Yan

et al. 2021

Front. Bioeng. Biotechnol.

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“…As monitored by TEM and ATPase activity, the disturbance of the integrity of the cell envelope by LA and AA leads to severe damage in A. baumannii . The decreased cellular ATPase activity of Na + /K + -ATPase and Ca 2+ /Mg 2+ -ATPase can reduce energy metabolism ( 42 , 43 ), thereafter aggravating the damage to A. baumannii cells. This also leads to an imbalance in osmotic pressure, which triggers the leakage of the cell content, apoptosis, and the disruption of cellular metabolism ( 44 , 45 ).…”

Section: Discussionmentioning

confidence: 99%