Possible Metabolic Pathways of Ethanol Responsible for Oxidative DNA Damage in Human Peripheral Lymphocytes

Yu, Yanying; Yang, Jingyu; Mou, Yanhua; Wang, Lihui; Zhang, Hong; Wu, Chunfu

doi:10.1111/j.1530-0277.2010.01316.x

Cited by 70 publications

(97 citation statements)

References 36 publications

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“…Our finding that DLMO by Method 1 was correlated with amount of drinking prior to the study suggests that perhaps there is a direct pathophysiological link between alcohol and circadian function, extending preclinical studies in mice that showed that forced ethanol consumption affects circadian activity in a dose-dependent manner (Brager et al 2010, 2011). Brager et al 2011 also found that acute alcohol consumption inhibits the phase resetting response of the mouse circadian clock to non-photic (serotonergic) phase resetting properties.…”

Section: Discussionsupporting

confidence: 70%

Dim Light Melatonin Onset in Alcohol-Dependent Men and Women Compared with Healthy Controls

Conroy

Hairston

Arnedt

et al. 2012

Chronobiology International

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Background Sleep disturbances in alcohol-dependent (AD) individuals may persist despite abstinence from alcohol and can influence the course of disorder. Although the mechanisms for their sleep disturbances are not well understood and some evidence suggests dysregulation of circadian rhythms, dim-light melatonin onset (DLMO) has not previously been assessed in AD vs. healthy control (HC) individuals in a sample that varied by sex and race. Methods Fifty-two AD participants (mean age 36.0 ± 11.0 years, 10 women) who were 3–12 weeks since their last drink (mean abstinence 57.9 ± 19.3 days) and 19 age- and sex-matched HCs (mean age 34.4 ± 10.6 years, 5 women) participated. Following a 23:00 – 06:00 h at-home sleep schedule for at least 5 days, and screening/baseline nights in the sleep laboratory, participants underwent a 3-hr extension of wakefulness (02:00 h bedtime) during which salivary melatonin samples were collected every 30 minutes beginning at 19:30 h. The time of DLMO was the primary measure of circadian physiology and was assessed with two commonly used methodologies. Results There was a slower rate of rise and a lower maximal amplitude in the AD group. DLMO varied by methodology used. Using 3 pg/ml as a threshold, no significant differences between the AD and HC groups were found. Using two standard deviations above the mean of the first 3 samples, AD DLMO occurred later 21:02 (SD=0:41) than HC 20:44 (SD=0:21) t=-2.4, (p=.02). Conclusions While melatonin in the AD group appears to have a slower rate of rise, using well-established criteria to assess salivary DLMO did not reveal differences between AD and HC participants. Only when capturing melatonin when it is already rising was DLMO significantly delayed by a mean 18 min in ADs. Future circadian analyses on alcoholics should account for these methodological caveats

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Section: Discussionsupporting

confidence: 70%

Dim Light Melatonin Onset in Alcohol-Dependent Men and Women Compared with Healthy Controls

Conroy

Hairston

Arnedt

et al. 2012

Chronobiology International

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show abstract

“…The cells were treated with 160 μM CoCl 2 for different times (15,30,60,120,180 and 360 min), then lysed with cold lysis buffer as described previously [24]. Cell lysate were electrophoresed on a 10% SDSPAGE gel, and transferred onto polyvinylidene difluoride membranes (Millipore Corporation, Bedford, MA).…”

Section: Western Blotting Analysismentioning

confidence: 99%

Effects of cobalt chloride on nitric oxide and cytokines/chemokines production in microglia

Mou¹,

Yang²,

Cui³

et al. 2012

International Immunopharmacology

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“…This shows an efficient repair mechanism of damaged DNA [55]. Further, it was found that DNA damage and intracellular 8-oxoguanine levels induced by ethanol can be 'auto-repaired' in human peripheral lymphocytes during a recovery phase [56]. It may be speculated that similar repair mechanisms are available in Caco-2 cells.…”

Section: Caco-2 Cellsmentioning

confidence: 95%

Genotoxic effects of camphorquinone and DMT on human oral and intestinal cells

et al. 2015

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Possible Metabolic Pathways of Ethanol Responsible for Oxidative DNA Damage in Human Peripheral Lymphocytes

Cited by 70 publications

References 36 publications

Dim Light Melatonin Onset in Alcohol-Dependent Men and Women Compared with Healthy Controls

Dim Light Melatonin Onset in Alcohol-Dependent Men and Women Compared with Healthy Controls

Effects of cobalt chloride on nitric oxide and cytokines/chemokines production in microglia

Genotoxic effects of camphorquinone and DMT on human oral and intestinal cells

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