Possible Protective Effects of Lutein against Ciprofloxacin Induced Bone Marrow Toxicity in Rats

Khudhair, Alaa Radhi; Al-Shawi, Nada N.

doi:10.31351/vol30iss1pp233-239

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…Twenty-four hours after DOX injection (i.e., at day 15), rats were anaesthetised using diethyl ether, blood samples were collected from the Jugular vein in non-heparinized tubes and were left to clot at room temperature, then centrifuged for 20 min at 4000 rpm to obtain serum and stored at -20°C for biochemical analysis; the animals were sacrificed by cervical dislocation, and the rats' femoral bone marrow (BM) was harvested and processed for genotoxicity evaluations (17).…”

Section: Animals and Experimental Designmentioning

confidence: 99%

Protective Effect of Dimethyl Fumarate on Doxorubicin-induced Genotoxicity in Rats

Al_kenany

Al-Shawi

2023

Preprint

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Background and Purpose Doxorubicin is a broad-spectrum antineoplastic agent; however, however, its genotoxic/cytotoxic effects limit its clinical application. Dimethyl fumarate (DMF) is an FDA-approved oral drug shown to have antioxidant, anti-inflammatory and antimutagenic effects via activating Nrf2 antioxidant pathway. The present study aimed to investigate the possible protective effect of DMF against doxorubicin-induced chromosomal and DNA damage in rat bone marrow cells. Experimental Approach Wistar Albino rats of both sexes were administered DMF orally (15mg/kg once daily for 14 days) alone or with doxorubicin which was injected as a single dose (90 mg/kg at day 14) to induce toxicity. The blood samples were collected 24 hours after doxorubicin’s injection from all groups to measure the serum levels of MDA, GSH, SOD, and GPx1 and bone marrow was harvested to assess chromosomal aberration, micronucleus, and comet assays. Key Results The rats in the doxorubicin-only group exhibited a significant decrease in mitotic index and depleted GSH and antioxidants enzymes serum levels with a significant elevation in MDA serum level, % DNA in Tail, micronucleus appearance and chromosomal aberrations compared to the control group; DMF pretreatment prior to doxorubicin exposure, significantly-reduced % DNA in Tail, micronucleus appearance, and chromosomal aberrations, improved mitotic index, restored GSH level and antioxidant enzymes activity compared doxorubicin-only group. Conclusion and Implication This study revealed that DMF alone has no DNA-damaging or clastogenic activities; DMF has protective effects against the genotoxicity induced by doxorubicin; thus, DMF might be a potential chemoprotective agent against doxorubicin-induced toxicity in cancer chemotherapy

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Section: Animals and Experimental Designmentioning

confidence: 99%

Protective Effect of Dimethyl Fumarate on Doxorubicin-induced Genotoxicity in Rats

Al_kenany

Al-Shawi

2023

Preprint

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“…Group IV: Each rat was orally-administered cafestol (5mg/kg/day) for 14 consecutive days, then a single dose of DOX (90mg/kg) was IP-injected-injected 1hour after the last cafestol treatment on day 14. Twenty-four hours after DOX injection (i.e., at day 15), rats were anaesthetized using diethyl ether and then sacrificed by cervical dislocation and the rats' femoral bone marrow (BM) was harvested and processed for genotoxicity evaluations (23) (24) .…”

Section: Animals and Experimental Designmentioning

confidence: 99%

Protective Effect of Cafestol on Doxorubicin-induced Genotoxicity in Rats

Sara A. Al-Kenany,

N. Al-Shawi

2023

IJPS

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Doxorubicin is an efficient antineoplastic agent that has a broad antitumour spectrum; however, its genotoxic adverse effects on normal cells can be produced through oxidative damage, and this limits its clinical application. Cafestol is a naturally-occurring diterpene in unfiltered coffee with noteworthy antioxidant, antimutagenic and anti-inflammatory activities. The present study aimed to investigate the possible protective effect of cafestol against doxorubicin-induced chromosomal and DNA damage in rat bone marrow cells. Wistar Albino rats of both sexes were administered cafestol (5mg/kg body weight once daily for 14 consecutive days) by oral gavage alone or with doxorubicin which was injected as a single dose (90 mg/kg intraperitoneally at day 14) to induce toxicity. The bone marrow was harvested 24 hours after doxorubicin’s injection in all groups for the assessment of structural chromosomal aberration, micronucleus, and comet assays. The result showed that rats in the doxorubicin-only group exhibited a significant decrease (P<0.05) in mitotic index with a significant elevation (P<0.05) in the % DNA in Tail, micronucleus appearance and total structural chromosomal aberrations compared to those of the negative control group; while oral administration of cafestol 14 days prior to doxorubicin, significantly-reduced the % DNA in Tail, micronucleus appearance, and the total number of chromosomal aberrations (P<0.05), and improved the mitotic index compared to rats intraperitoneally-injected with doxorubicin alone. This study revealed that cafestol has protective effects against the genotoxicity induced by doxorubicin.

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تقييم نشاط التئام الجروح وتحفيز موت الخلايا المبرمج لمشتقات الكينازولينون الجديدة

Oleiwi,

Zalzala,

Khudhair

et al. 2024

Al-Rafidain J Med Sci

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Background: Chemotherapeutic medication treatment for cancer is typically used in conjunction with other techniques as part of a routine regimen. It is well established that the capacity of different chemotherapeutic drugs to induce apoptosis is correlated with their anticancer efficacy. Quinazolinone-based drugs have demonstrated excellent responses from several cancer cell types. These substances have a lot of potential for use as building blocks in the creation of apoptosis inducers. Objective: To assess the new quinazolinone derivatives (M1 and M2) that were recently synthesized for their potential to halt wound healing and to use the acridine orange/propidium iodide (AO/PI) double stain to assess their capacity to induce apoptosis in the chosen cancer cell lines. Methods: Using the breast carcinoma cell line (MCF-7) and the lung adenocarcinoma cell line (A549), two quinazolinone derivatives (M1 and M2) were investigated for their capacity to inhibit wound healing and induce apoptosis. Results: In both cell lines, the chemicals were found to be effective inducers of apoptosis and to considerably limit wound healing. Conclusions: In cancer cell lines (MCF-7 and A549), compounds M1 and M2 efficiently inhibited wound repair and triggered apoptosis.

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Possible Protective Effects of Lutein against Ciprofloxacin Induced Bone Marrow Toxicity in Rats

Cited by 3 publications

References 22 publications

Protective Effect of Dimethyl Fumarate on Doxorubicin-induced Genotoxicity in Rats

Protective Effect of Dimethyl Fumarate on Doxorubicin-induced Genotoxicity in Rats

Protective Effect of Cafestol on Doxorubicin-induced Genotoxicity in Rats

تقييم نشاط التئام الجروح وتحفيز موت الخلايا المبرمج لمشتقات الكينازولينون الجديدة

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