Possible regulation of genes associated with intracellular signaling cascade in rat liver regeneration

Abstract

ObjectiveThe importance of signal transduction in cell activities has been generally accepted. The purpose of this study was to analyze the regulatory effect of intracellular signaling cascade-associated genes on rat liver regeneration (LR) at transcriptional level.Material and methodsThe associated genes were originally obtained through a search of the databases and related scientific publications; their expression profiles were then checked in rat LR using the Rat Genome 230 2.0 array. The LR-associated genes were identified by comparing the discrepancy in gene expression changes between the partial hepatectomy (PH) group and the sham operation (SO) group.ResultsA total of 566 genes associated with the intracellular signaling cascade were LR related. The genes involved in nine signaling pathways including intracellular receptor-, second messenger-, nitric oxide-, hormone-, carbohydrate-mediated, protein kinase, small GTPase, ER-nuclear and target of rapamycin (TOR) signaling pathways were detected to be enriched in a cluster characterized by up-regulated expression in LR. According to their expression similarity and time relevance, they were separately classified into 5 and 5 groups.ConclusionsIt is presumed that following PH, the second messenger-mediated signaling pathway inhibits the inflammatory response, while the protein kinase cascade and small GTPase-mediated signal transduction stimulate the immune response; the intracellular receptor-, second messenger-, small GTPase-mediated signal transduction and protein kinase cascade coordinately control cell replication; the intracellular receptor-, second messenger-mediated and ER-nuclear signaling pathways facilitate cell differentiation; the MAPK cascade and small GTPase-mediated signal transduction play a role in cytoskeletal reconstruction and cell migration; the second messenger-, small GTPase-mediated and IκB kinase/NFκB cascades take care of protein transport, etc., in LR.

Effect of leucine on NF-κB pathway in liver regeneration after partial hepatectomy in rats, as determined by miniarray analysis

“…Though the role played by angiotensinogen (AGT) after PH is not well known, it was reported to peak at 6th hour after PH and to maintain its expression up to 24 hours (25). Xu et al revealed a 5-fold increase of AGT expression (37). This is consistent with our fi nding showing a 5-fold increase of AGT in the Group II than that in the Group I.…”

Lipocalin-2 gene expression during liver regeneration after partial hepatectomy in rats

“…29 The regulation of genes associated with intracellular signaling cascades is highly suspected to be involved in the mechanism of liver regeneration. 30 The ratio of remnant liver weight to body weight increased to near 90% of the original liver weight at 72 h after PH. As shown by the BrdU proliferation ELISA assay, proliferation of the isolated hepatocytes was markedly induced by PH on day 3 and day 4.…”

<p>Knockdown of SLC35F2 Inhibits the Proliferation and Metastasis of Bladder Cancer Cells</p>

“…Inhibitors and monoclonal antibodies can target the EGFR and TGF-β signaling pathways in breast cancer therapy 22. Small GTPase-mediated signal transduction can stimulate the immune response, control cell replication, play a role in cytoskeletal reconstruction and cell migration, and participate in protein transport 23. In the KEGG pathway analysis, the coexpressed genes were mainly enriched in BC, the calcium signaling pathway, the cAMP signaling pathway, the GnRH signaling pathway, arachidonic acid metabolism and the Rap1 signaling pathway.…”