Possible role of carbonic anhydrase, V–H+–ATPase, and Cl−/HCO3− exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus

Genovese, Griselda; Ortiz, Nicolás; Urcola, Matías R.; Luquet, Carlos Marcelo

doi:10.1016/j.cbpa.2005.08.024

Cited by 48 publications

(40 citation statements)

References 49 publications

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“…This implies a possible involvement of other enzymes in osmoregulation. In the bimodal crab, C. granulatus, HA was more important than NKA in a DSW environment (Genovese et al, 2005). This was also supported by research on the other two freshwater crabs, Dilocarcinus pagei (Onken and McNamara, 2002) and Eriocheir sinensis (Onken and Putzenlechner, 1995;Morris, 2001).…”

Section: Discussionmentioning

confidence: 75%

“…Furthermore, apical HA may play an important role in freshwater osmoregulation of crabs from the marine environment onto land (Weihrauch et al, 2004;Genovese et al, 2005). Several recent studies have focused on the osmoregulatory role of HA in some euryhaline or freshwateracclimated crabs, such as Carcinus maenas (Weihrauch et al, 2002), Dilocarcinus pagei (Weihrauch et al, 2004), Chasmagnathus granulatus (Genovese et al, 2005) and Eriocheir sinensis (Onken and Putzenlechner, 1995;Riestenpatt et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Several recent studies have focused on the osmoregulatory role of HA in some euryhaline or freshwateracclimated crabs, such as Carcinus maenas (Weihrauch et al, 2002), Dilocarcinus pagei (Weihrauch et al, 2004), Chasmagnathus granulatus (Genovese et al, 2005) and Eriocheir sinensis (Onken and Putzenlechner, 1995;Riestenpatt et al, 1995). Nevertheless, only a few studies have measured HA activity.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, only a few studies have measured HA activity. Most of them determined the functions of HA using electrophysiological methodology (Onken and McNamara, 2002;Genovese et al, 2005) or mRNA expression . A direct measurement of HA activity and its cytological distribution in the gill would be powerful support for the correlation of function, distribution and adaptation.…”

Section: Introductionmentioning

confidence: 99%

“…According to the distribution of HA, these 13 species were classified into three groups for further analyses on the RSRs of NKA. An upregulation of NKA is an indication of the individual's dependence on NKA to overcome hypotonic stress (Genovese et al, 2005). If the crab maintains stable NKA activity, HA activity is expected to change.…”

Section: Introductionmentioning

confidence: 99%

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V-type H+-ATPase and Na+,K+-ATPase in the gills of 13 euryhaline crabs during salinity acclimation

Tsai

Lin

2007

Journal of Experimental Biology

127

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SUMMARY Because of their diverse habitats, crabs are excellent experimental species to study owing to the morphological changes and physiological adaptation that occur during their terrestrial invasion. Their hemolymphic osmoregulation in brackish water is crucial for a successful terrestrial invasion. Crabs can actively uptake or excrete ions upon salinity change, and the gills play a major role among the osmoregulatory organs. Several enzymes are involved in the osmoregulatory process, including Na+,K+-ATPase and V-type H+-ATPase (V-H+-ATPase). Na+,K+-ATPase is the driving force in establishing an ion gradient across the epithelial cell membrane in marine crabs. It has been reported that the osmoregulatory mechanisms in freshwater crabs are different from those in marine ones, suggesting that the driving force may come from V-H+-ATPase by generating the H+ ion gradient to facilitate the ion flow. Thirteen crab species from two families were used in this study. These crabs lived in five different habitats, including marine,intertidal, bimodal, freshwater and terrestrial habitats. The distribution of V-H+-ATPase in the 13 euryhaline crabs was revealed by histochemistry. V-H+-ATPase was localized in the apical region in crabs that could survive in the freshwater environment. We found that the freshwater and terrestrial crabs with stable Na+,K+-ATPase activity during salinity changes tended to have an apical V-H+-ATPase, whereas the intertidal ones with varying Na+,K+-ATPase activity showed a cytoplasmic V-H+-ATPase distribution. Finally, in Uca formosensis, a crab that had stable Na+,K+-ATPase activity, a significant difference in V-H+-ATPase activity between salinities was found. In conclusion, the hypothesis that V-H+-ATPase plays a crucial role in the freshwater adaptation of crabs is supported by our systemic investigation on 13 euryhaline crabs.

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Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

V-type H+-ATPase and Na+,K+-ATPase in the gills of 13 euryhaline crabs during salinity acclimation

Tsai

Lin

2007

Journal of Experimental Biology

127

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Hemolymph composition, gene expressions in the gills, and thus the survival of euryhaline crabs are controlled by ambient minor cations according to osmotic condition‐dependent manner

Yamaguchi

Soga

2020

Ecology and Evolution

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Alterations in Hemolymph Ion Concentrations and pH in Adult Daphnia magna in Response to Elevations in Major Ion Concentrations in Freshwater

Morris

Sakarya

Koh

et al. 2020

Enviro Toxic and Chemistry

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Increases in the concentrations of major ions (Na+, K+, Ca2+, Mg2+, Cl−) in freshwater are a growing concern for ecosystem health. These increases may originate from anthropogenic activities such as road deicing, fracking spills, mining, and fertilizer application and have detrimental effects on freshwater organisms through disturbances in ionoregulation and acid–base balance. The cladoceran Daphnia magna is adapted for active ion uptake and reduction of ion loss to maintain osmotic balance, but alterations in ionic composition of the environmental water are associated with toxicity. In the present study, hemolymph ion concentrations were measured using ion‐selective microelectrode techniques. Increases in the hemolymph concentrations of Na+ and K+ correspond to elevations in the concentrations of these ions in ambient water. Water concentrations associated with sustained increases in hemolymph ion concentrations correlate well with median lethal concentration values from previous toxicology studies, indicating that Na+ and K+ concentrations in hemolymph may predict toxicity. When water K+ concentration is increased, a simultaneous increase in water Na+ concentration mitigates the increase in hemolymph K+ concentration, a finding which is consistent with the reported mitigation of K+ toxicity by Na+. When ambient concentrations of K+, Na+, and Cl− are increased, not only is there a rise in hemolymph ion concentration but hemolymph pH is altered and pH regulation appears to be prioritized over regulation of hemolymph Na+, K+, and Cl− in D. magna. Environ Toxicol Chem 2021;40:366–379. © 2020 SETAC

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Possible role of carbonic anhydrase, V–H+–ATPase, and Cl−/HCO3− exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus

Cited by 48 publications

References 49 publications

V-type H+-ATPase and Na+,K+-ATPase in the gills of 13 euryhaline crabs during salinity acclimation

V-type H+-ATPase and Na+,K+-ATPase in the gills of 13 euryhaline crabs during salinity acclimation

Hemolymph composition, gene expressions in the gills, and thus the survival of euryhaline crabs are controlled by ambient minor cations according to osmotic condition‐dependent manner

Alterations in Hemolymph Ion Concentrations and pH in Adult Daphnia magna in Response to Elevations in Major Ion Concentrations in Freshwater

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