Possible role of non-bilayer lipids in the structure of mitochondria. A freeze-fracture electron microscopy study

Venetië, R.; Verkleij, Arie J.

doi:10.1016/0005-2736(82)90390-x

Cited by 120 publications

(46 citation statements)

References 26 publications

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“…Such structures have also been found in the adrenal cells and the retinal ganglia cells in which liposidosis is chemically induced [62]. They can be induced in vitro in the matrix of isolated mitochondria upon addition of Mn 2÷ [63] and can be found between thylakoid membranes upon heating above 45°C [64]. Although the biological relevance of these structures is as yet unknown, it indicates that the bilayer organization of lipids in biomembranes is not a stable one.…”

Section: Va Hi1 Phasementioning

confidence: 99%

“…Biochemical evidence supporting this proposal comes from studies on the ATP-ADP translocation [75] and the transport of lipid molecules from outer to inner mitochondrial membrane in the presence of Ca 2+ [76]. Freeze-fracture studies [63] have shown that conditions which favour Hil phase formation of lipids (including the addition of Ca 2 +) induce a patchwork-like structure, as a result of a frequent jumping of the fracture plane between the outer and inner mitochondrial membranes (Fig. 9).…”

Section: Vb Lipidic Particles and Related Structuresmentioning

confidence: 99%

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Lipidic intramembranous particles

Verkleij

1984

Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

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411

166

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Section: Va Hi1 Phasementioning

confidence: 99%

Section: Vb Lipidic Particles and Related Structuresmentioning

confidence: 99%

Lipidic intramembranous particles

Verkleij

1984

Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

Self Cite

411

166

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“…3 Structure of VDAC-ANT complex in the contact site. (A) Two dimers of VDAC and ANT are connected by non bilayer cardiolipin arranged as inverted micelle according to [100] (shown in B) or as opening to the cytosol. This arrangement would explain how inner membrane cardiolipin (diphosphatidyl glycerol DPG) could come to the mitochondrial surface and cytochrome c could be detached from its binding sites in the contact sites by external Bax without forming pores.…”

Section: Cholesterol and Cardiolipin Influence Vdac Structure/functionmentioning

confidence: 99%

“…3. According to the model of Van Venetie and Verkleij [100] cardiolipin may form inverted micelles or arrange in other non bilayer structures. The authors postulated formation of contact sites by pure non bilayer phospholipid arrangement induced by Ca 2+ .…”

Section: Cholesterol and Cardiolipin Influence Vdac Structure/functionmentioning

confidence: 99%

The permeability transition pore in cell death

2007

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The permeability transition pore (PT-pore) is a multi-component protein aggregate in mitochondria that comprises factors in the inner as well as in the outer mitochondrial membrane. This complex has two functions: firstly, it regulates the integration of oxidative phosphorylation into the cellular energy household and secondly, it induces cell death when converted into an unspecific channel. The latter causes a collapse of the mitochondrial membrane potential and activates a chain of events that culminate in the demise of the cell. It has been controversial for some time whether the PT-pore is causative for or only amplifies a signal of cell death but novel results confirm a central role of this protein complex for cell death induction. While a considerable body of data exist on its subunit composition, recent genetic knock-out experiments suggest that the identity of the core factors of the PT-pore is still unresolved. Moreover, accumulating evidence point to a much more complex composition of this protein complex than anticipated. Here, we review the current knowledge of its subunit composition, the evidence of a role in cell death, and we propose a model for the activation of the PT-pore for cell death. The role of the PT-pore in apoptosisThe permeability transitionThe permeability transition (PT) is a sudden and sustained increase of the permeability of the inner mitochondrial membrane for solutes smaller than 1.5 kD. This has catastrophic consequences for this organelle. The mitochondrial membrane potential m , which relies on the im-permeability of the inner membrane for protons, breaks down and with it the ability of the cell to synthesize ATP. The ensuing blockade of the respiratory chain leads to the generation of reactive oxygen intermediates (ROIs), most likely via the direct transfer of electrons to molecular oxygen. Also, the high concentration of solutes in the mitochondrial matrix generates an osmotic pressure, which drives the influx of water molecules and the expansion of the extensively folded inner membrane and eventually disrupts the outer membrane leading to the release of factors that execute the suicide programme of apoptosis. Thus, the self-destruction of mitochondria via the PT is followed by the demise of the cell as a whole. This process of mitochondrial destruction was first observed in vitro but was dismissed at the time as an artefact because, among other reasons, it was inconceivable why mitochondria should dispose of a process to dismantle themselves. This changed, however, when it was shown that the PT is mediated by a protein complex, the so-called permeability transition pore (PT-pore), and that various physiological and pharmacological reagents can act on this pore and modify the deadly response of mitochondria. Finally, the realisation that cells can mount an active suicide response that is mediated in large part by mitochondria placed the PT-pore firmly on the map of researchers. In particular, the use of cyclosporine A (CsA) that can inhibit the PT-pore subunit cyc...

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“…The Hn phase was first observed in the X-ray diffraction studies of Luzzati and coworkers (Luzzati & Reiss-Hudson, 1962;Luzzati & Reiss-Hudson, 1966;Luzzati et al, 1968;Luzzati & Tardieu, 1974) and the technique has since been employed by many others (Rand et al, 1971;Harlos & Eible, 1981;Gruner et al, 1988). The Hn phase has also be detected by 31 P-NMR (Cullis & de Kruijff, 1978;Cullis & de Kruijff, 1979); 2H-NMR (Tilcock et al, 1982); freeze-fracture electron microscopy (Deamer et al, 1970;de Kruijff et al, 1979;Van Venetie & Verkleij, 1981); fluorescence (Ellens et al, 1986a;Bentz et al, 1987;Hong et al, 1988); IR spectroscopy (Mantsch et al, 1981); ESR spectroscopy (Hardman, 1982); and differential scanning 44 calorimetry (Harlos & Eible, 1981;Seddon et al, 1983a;Epand, 1985;Ellens et al, 1986a;Ellens et al, 1986b). Hn phases have been directly visualized by both freeze fracture electron microscopy (Deamer et al, 1970;Hui & Stewart, 1981) and cryo-transmission electron microscopy Talmon et al, 1990).…”

Section: Ib3 Nonlamellar Phase Transitionsmentioning

confidence: 97%

Photoinduced Fusion of Liposomes

Bennett¹,

O’Brien²

1994

J. Am. Chem. Soc.

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This manuscript ,has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissenation copies are in typewriter face, while others may be from any type of computer printer.The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction.In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note wiD indicate the deletion.Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continning from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book.

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Possible role of non-bilayer lipids in the structure of mitochondria. A freeze-fracture electron microscopy study

Abstract: The possible role of non-bilayer phospholipids on the structure of isolated rat liver mitochondria has been morphologically studied.

Cited by 120 publications

References 26 publications

Lipidic intramembranous particles

Lipidic intramembranous particles

The permeability transition pore in cell death

Photoinduced Fusion of Liposomes

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