Post-column fluorometric detection of reducing sugars in high performance liquid chromatography using arginine.

Mikami, Hirohisa; Ishida, Yasuo

doi:10.2116/bunsekikagaku.32.6_e207

Cited by 180 publications

(84 citation statements)

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“…The cell wall peptidoglycan is based on D-ornithine or D-hydroxyornithine (type B2P of Schleifer and Kandler bers of isoprene units (10,11,12,13, and 14 isoprene units). The polar lipids are diphosphatidylglycerol, phosphatidylglycerol, and unidentified glycolipids.…”

Section: Resultsmentioning

confidence: 99%

Proposal of Six New Species in the Genus Aureobacterium and Transfer of Flavobacterium esteraromaticum Omelianski to the Genus Aureobacterium as Aureobacterium esteraromaticum comb. nov.

Yokota

Takeuchi²,

Sakane³

et al. 1993

International Journal of Systematic Bacteriology

121

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Twelve strains placed in the genera Flavobacterium, Pseudomonas, and Aureobacterium, including soil isolates, were characterized taxonomically. On the basis of morphological, physiological, and chemotaxonomic data, as well as DNA-DNA hybridization data, we propose that 11 of these strains should be classified in the genus Aureobacterium as new combinations or new species, as follows: Aureobacterium esteraromaticum comb. nov. ( The genus Aureobacterium was proposed by Collins et al. (2), and the following six species have been described previously (8): Aureobacterium liquefaciens, Aureobacterium jlavescens, Aureobacterium terregens, Aureobacterium saperdae, Aureobacteriurn barkeri, and Aureobacterium testaceurn.During the course of a taxonomic study of the Flavobacterium strains in the Institute for Fermentation at Osaka (IFO) culture collection, we found that the aromatic compound producers Flavobacterium esteraromaticum (18, 23) and "Flavobacterium suaveolens" (24) and the keratan sulfate endo-P-galactosidase (keratanase) producer Pseudomonas sp. strain IF0 14344= (T = type strain) (16, 17) were members of the genus Aureobacterium (26). To determine the taxonomic position of these organisms, we examined their physiological and chemotaxonomic characteristics, as well as the characteristics of some soil isolates and strains designated Aureobacterium sp., and compared our results with data for previously described species of the genus Aureobacterium.In this paper we describe the characteristics of 12 strains of Aureobacterium species. We propose six new species and one new combination in the genus Aureobacterium for these strains on the basis of morphological, physiological, biochemical, and DNA-DNA hybridization data. MATERIALS AND METHODSBacterial strains and culture conditions. The bacterial strains which we studied are listed in Table 1. Biomass for the chemical analyses was prepared by growing the strains at 28°C with aerobic shaking in PY medium supplemented with Bacto brain heart infusion (Difco Laboratories, Detroit, Mich.) (PY-BHI medium), which contains 1% peptone, 0.2% yeast extract, 0.2% brain heart infusion, 0.2% NaCl, and * Corresponding author. 5550.2% D-glucose (pH 7.0). Cells were harvested by centrifugation, washed with water, and lyophilized.Morphological, physiological, and biochemical characteristics. Unless otherwise indicated, all of the tests were carried out at 28°C. Cell morphology was determined by examining cells grown on PY-BHI agar. Motility was determined by the hanging drop method. Catalase activity was determined by bubbling in a 3% hydrogen peroxide solution. Oxidase activity was determined by the oxidation of 1% tetramethylp-phenylenediamine on filter paper. Acid production from carbohydrates was studied in a medium containing 0.3% peptone, 0.25% NaC1, 0.003% bromcresol purple, and 0.5% carbohydrate (pH 7.2) (31). Assimilation of organic acids was studied in a medium containing 0.5% organic acid (sodium salt), 0.02% D-glucose, 0.01% yeast extract, 0.01% Trypticase (BBL), 0.1% ...

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Section: Resultsmentioning

confidence: 99%

Proposal of Six New Species in the Genus Aureobacterium and Transfer of Flavobacterium esteraromaticum Omelianski to the Genus Aureobacterium as Aureobacterium esteraromaticum comb. nov.

Yokota

Takeuchi²,

Sakane³

et al. 1993

International Journal of Systematic Bacteriology

121

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“…The amino-acid and sugar compositions of fraction-A (Figure 1c; F-A) were analysed by the ninhydrin method (Moore and Stein, 1948) and post-column fluorometric detection (Mikami and Ishida, 1983), respectively.…”

Section: Amino-acid and Sugar-composition Analysesmentioning

confidence: 99%

Extracellular polysaccharide-protein complexes of a harmful alga mediate the allelopathic control it exerts within the phytoplankton community

et al. 2009

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The goal of this study was to examine the significance of allelopathy by the raphidophyte Heterosigma akashiwo in a multispecies phytoplankton community in the field. Towards this aim, we sought allelochemicals of H. akashiwo, which had allelopathic effect both in laboratory experiments and in the field. As an initial step, we showed that the allelopathic effects of H. akashiwo filtrate were both species-specific and dependent upon the cell density of the target species. Secondly, we found for the first time that extracellular, high-molecular weight allelochemicals [that is, polysaccharideprotein complexes (APPCs)] were produced by a marine phytoplankton species, H. akashiwo. Thirdly, we indicated that the purified APPCs selectively inhibited the growth of the diatom Skeletonema costatum that is a major competitor of H. akashiwo, and thereby tended to promote the formation of monospecific H. akashiwo blooms. Furthermore, we demonstrated that the inhibitory effect of APPCs on the growth of the diatoms was determined by binding to the cell surface of the target species. Finally, we succeeded in the detection of APPCs in the field samples at concentrations exceeding their experimentally determined action threshold during the H. akashiwo bloom. Strategies for ecosystem control, including mitigation of harmful algal blooms (HABs), should take into account that red-tide organisms like H. akashiwo are already part of complex webs involving inter-specific allelopathic inhibition and ecosystem control during their dense blooms.

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“…The amounts of amino sugars were determined by amino acid analyzer after hydrolysis with 4 M HC1 at 100°C for 4 h. After hydrolysis with 2.5 M trifluoroacetic acid at 100°C for 6 h [28], neutral sugars were analyzed together with N-acetylglucosamine by high-performance liquid chromatography (HPLC) using a Shimadzu ISA-O7/S2504 column [29]. AChR was hydrolyzed with 0.1 M trifluoroacetic acid at 80 "C for 1 h and was subjected to the 2-thiobarbituric acid method for the assay of sialic acid [30].…”

Section: Carbohydrate Compositionmentioning

confidence: 99%

Carbohydrate structures of acetylcholine receptor from Torpedo californica and distribution of oligosaccharides among the subunits

et al. 1986

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The structure of carbohydrates in acetylcholine receptor (AChR) from Torpedo californica is reported. Oligosaccharides released quantitatively from the whole molecule by N-oligosaccharide glycopeptidase digestion were fractionated by thin-layer chromatography and further purified by high-performance liquid chromatography. We show that more than 70% of the total oligosaccharide chains in Torpedo AChR are of the high-mannose type with the structures (Man)8(GlcNAc)z and (Man)g(GlcNAc),. The structure of these oligosaccharides were determined by proton nuclear magnetic resonance spectroscopy. These two types of oligosaccharides were shown to be distributed in different proportions in all subunits of Torpedo AChR. We also show that several kinds of complex-type oligosaccharides comprising the rest of the carbohydrate in the protein exist mainly in the y and 6 subunits. The structure of the carbohydrate moiety that is distributed on the four subunits of AChR was also examined by susceptibility to endo-P-N-acetylglucosaminidase and sialidase and by binding affinity to lectins, e.g. concanavalin A, leucoagglutinating phytohemagglutinin, and wheat germ agglutinin.The nicotinic acetylcholine receptor (AChR) is distributed in mammalian skeletal muscles and electroplaques of electric fishes [l-31. It is known that AChR is composed of four different homologous glycosylated subunits (a, P, y, and 8) in a molar stoichiometry of aZPy6.

show abstract

Post-column fluorometric detection of reducing sugars in high performance liquid chromatography using arginine.

Cited by 180 publications

References 0 publications

Proposal of Six New Species in the Genus Aureobacterium and Transfer of Flavobacterium esteraromaticum Omelianski to the Genus Aureobacterium as Aureobacterium esteraromaticum comb. nov.

Proposal of Six New Species in the Genus Aureobacterium and Transfer of Flavobacterium esteraromaticum Omelianski to the Genus Aureobacterium as Aureobacterium esteraromaticum comb. nov.

Extracellular polysaccharide-protein complexes of a harmful alga mediate the allelopathic control it exerts within the phytoplankton community

Carbohydrate structures of acetylcholine receptor from Torpedo californica and distribution of oligosaccharides among the subunits

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