Background: The present study aims to investigate the effects of patient safety culture on the prevention of transmitting bloodborne pathogens among nurses at a general hospital. Methods: The participants were 284 nurses working at a general hospital located in P city, and the data were collected between April 26 and May 15, 2019. Questionnaires on patient safety culture and prevention of bloodborne pathogens were used, and the data were examined on SPSS version 22.0 using frequency analysis, percentages, mean, standard deviation, t-test, ANOVA, Pearson’s correlation, and hierarchical regression analysis. Results: The results showed that the following factors affected the prevention of bloodborne pathogens: experience of needle stick and sharp injuries (β=-0.94), teamwork (β=0.41), knowledge and attitude toward patient safety (β=0.34), leadership (β=0.15), and priority of patient safety (β=0.14). The model's explanatory power was 53% (F=32.26, p=<0.001).Conclusions:In order to increase the compliance of general hospital nurses with the practices that promote prevention of bloodborne pathogens, it is necessary to actively prevent needle stick and sharp injuries.It is also necessary to prioritize patient safety, and to develop and verify the effects of various programs that emphasize factors of patient safety culture, such as leadership, teamwork, knowledge, and attitude.