Post-hatch Changes in the Immunoexpression of Desmin, Smooth Muscle Actin and Vimentin in the Testicular Capsule and Interstitial Tissue of the Japanese quail (Coturnix coturnix japonica)

Abstract

Summary The post‐hatch development of immunoreactivity to desmin, smooth muscle actin (SMA) and vimentin in the testicular capsule and interstitial tissue of day‐old to adult quails was described in this study. The tunica albuginea of the testicular capsule was composed mainly of myoid cells. A zonal arrangement of desmin and SMA immunostaining was observed in myoid cells of the tunica albuginea in 1‐ to 24‐day‐old quails. Immunostaining for SMA and desmin was uniform in the tunica albuginea of adult birds. Vimentin immunostaining in the testicular capsule was demonstrated in mesothelial cells, endothelial cells and fibroblasts. The interstitial tissue contained mesenchymal cells, peritubular myoid cells, Leydig cells and fibroblasts. Desmin‐immunopositive mesenchymal cells were present in the interstitial tissue of 1‐ to 17‐day‐old quails. Peritubular myoid cells expressed strong desmin immunostaining in all developmental stages, while the intensity of SMA immunostaining increased with testicular maturation. Vimentin was demonstrated in Leydig cells and fibroblasts, while the peritubular myoid cells displayed strong vimentin immunostaining only in adult birds. Strong vimentin immunostaining was demonstrated in the endothelial cells of capsular and interstitial blood vessels. The tunica media of these blood vessels displayed desmin and SMA immunostaining. The results of the study have established that variability exists in the distribution and intensity of desmin, SMA and vimentin immunostaining in the testicular capsule and interstitial tissue of the post‐hatch Japanese quail.

Comparison of Formation of Capsule Among Different Breast Silicone Implants

“…High levels of Vimentin and alpha-SMA expression indicate that more collagen was generated during this period. The capsule can be thick, more likely to shrink, and finally cause capsular contracture (32,34). α-SMA is a differentiated myofibroblast maker whose expression in the capsule is measured using IF staining.…”

Biofunctionalization of silicone rubber with microgroove-patterned surface and carbon-ion implantation to enhance biocompatibility and reduce capsule formation

“…High levels of α-SMA and vimentin usually lead to capsular contracture. 26 We found that α-SMA and vimentin expression gradually increased with time for all implant materials studied, but the expression with PC-SR was lower than with the other materials. It appears that the combination of C-ion implantation and surface microgroove patterning of silicone rubber reduces inflammatory cell infiltration and collagen deposition, delaying the occurrence of capsular contracture, although the underlying mechanism needs further indication.…”

Seasonal cycle of leydig cells in the Japanese Jungle Crow (Corvus macrorhynchos)

“…Concomitant with the atrophy of the exhausted generation, a new generation of juvenile interstitial cells begins to arise, presumably by their differentiation from the fibroblast-like progenitors (Nicholls and Graham, 1972), and gradually begins to mature. An immunohistochemical study demonstrates a similarity between fibroblasts of interstitium and the Leydig cells in such a way that vimentin is expressed both in Leydig cells and in fibroblasts during maturation of Japanese quails (Coturnix coturnix japonica) ((Madekurozwa, 2013).Concluding, seasonal testicular regression in seasonally breeding birds is mediated by apoptosis (Young et al, 2001). The seasonal replacement by the new interstitial cells at the end of each breeding period is not unique to avian testicular cycles.…”