Post-Injury and Resolution Response to Repetitive Inhalation Exposure to Agricultural Organic Dust in Mice

Warren, Kristi J.; Wyatt, Todd A.; Romberger, Debra J.; Ailts, Isaak; West, William W.; Nelson, Amy; Nordgren, Tara M.; Staab, Elizabeth; Heires, Art J.; Poole, Jill

doi:10.3390/safety3010010

Cited by 19 publications

(18 citation statements)

References 49 publications

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“…All populations were gated by forward- and side-scatter properties characteristic of lymphocytes (SSC-FSC + ) and granulocytes (SSC + FSC lo ) and antibody-specific staining fluorescence intensity. The gating strategy after removal of debris, doublets, and selection of CD45 + cells was CD3 + CD19 − T cells, CD3 − CD19 + B cells, and CD3 − CD19 − CD11c hi conventional DC, SSC + Autoflourescence + CD11c + macrophages, and CD3 − NK1.1 + natural killer cells (NK cells), CD11c − CD11b + Siglec − F + eosinophils, CD11c − Ly6G + neutrophils as previously published [27, 28]. Group 2 innate lymphoid cells (ILC2) were gated as lineage (LIN) negative (CD3 − CD19 − CD11b − CD11c − NK1.1 − ) and inclusion of ICOS + ST2 + , and ILC3 were gated as LIN − NKp46 + .…”

Section: Methodsmentioning

confidence: 99%

Ovalbumin-sensitized mice have altered airway inflammation to agriculture organic dust

et al. 2019

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Agriculture exposures are associated with reducing the risk of allergy and asthma in early life; yet, repeated exposures later in life are associated with chronic bronchitis and obstructive pulmonary diseases. The objective of this study was to investigate the airway inflammatory response to organic dust extract (ODE) in mice with established ovalbumin (OVA)-induced experimental asthma. C57BL/6 mice were either OVA sensitized/aerosol-exposed or saline (Sal) sensitized/aerosol-challenged. Both groups were then subsequently challenged once with intranasal saline or swine confinement ODE to obtain 4 treatment groups of Sal-Sal, Sal-ODE, OVA-Sal, and OVA-ODE. Airway hyper-responsiveness (AHR) to methacholine, bronchiolar lavage fluid, lung tissues, and serum were collected. Intranasal inhalation of ODE in OVA-treated (asthmatic) mice (OVA-ODE) increased AHR and total cellular influx marked by elevated neutrophil and eosinophil counts. Flow cytometry analysis further demonstrated that populations of CD11c hi dendritic cells (DC), CD3 + T cells, CD19 + B cells, and NKp46 + group 3 innate lymphoid cells (ILC3) were increased in lavage fluid of OVA-ODE mice as compared to ODE or OVA alone. Alveolar macrophages, DC, and T cells were significantly increased with co-exposure to OVA-ODE as compared to OVA alone. Lung ILC2 and ILC3 were only increased in OVA-Sal mice. Cytokine/chemokine levels varied with exposure to OVA-ODE reflecting an additive mixture of the pro- and allergic-inflammatory profiles. Collectively, ODE increased airway inflammatory cells and chemotactic mediator release in allergic (OVA) sensitized mice to suggest that persons with allergy/asthma be identified and warned prior to the occupational exposure of potentially worsening airway disease. Electronic supplementary material The online version of this article (10.1186/s12931-019-1015-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Ovalbumin-sensitized mice have altered airway inflammation to agriculture organic dust

et al. 2019

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“…Lung lymphocytes were isolated from homogenized lung tissue for the intracellular staining experiments using the method described previously (33). For the IL-33 and IL-23 cell culture experiments, lung ILC2 sorting was conducted as previously published (34), and pan innate lymphoid cells were enriched using StemCell pan ILC enrichment kit (Tempe, AZ). Brie y, lung tissue was dissociated, processed, and labeled with antibodies to common immune lineage markers, as described in the previous section.…”

Section: Lung Lymphoid Cell Isolation and Activationmentioning

confidence: 99%

Neutralization of IL-33 Modifies the Type 2 and Type 3 Inflammatory Signature of Viral Induced Asthma Exacerbation.

Warren

Poole

Sweeter

et al. 2021

Preprint

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Background: Respiratory viral infections are one of the leading causes of need for emergency care and hospitalizations in asthmatic individuals, and airway-secreted cytokines are released within hours of viral infection to initiate these exacerbations. IL-33, specifically, contributes to these allergic exacerbations by amplifying type 2 inflammation. We hypothesized that blocking IL-33 in RSV-induced exacerbation would significantly reduce allergic inflammation. Methods: Sensitized BALB/c mice were challenged with aerosolized ovalbumin (OVA) to establish allergic inflammation, followed by RSV-A2 infection to yield four treatment groups: Saline only (Saline), RSV-infected alone (RSV), OVA alone (OVA), and OVA-treated with RSV infection (OVA-RSV). Lung outcomes included lung mRNA and protein markers of allergic inflammation, histology for mucus cell metaplasia and lung immune cell influx by cytospin and flow cytometry. Results: While thymic stromal lymphopoietin (TSLP) and IL-33 were detected 6 hours after RSV infection in the OVA-RSV mice, IL-23 protein was uniquely upregulated in RSV-infected mice alone. OVA-RSV animals varied from RSV- or OVA-treated mice as they had increased lung eosinophils, neutrophils, group 2 innate lymphoid cells (ILC2) and group 3 innate lymphoid cells (ILC3) detectable as early as 6 hours after RSV infection. Neutralized IL-33 significantly reduced ILC2 and eosinophils, and the prototypical allergic proteins, IL-5, IL-13, CCL17 and CCL22 in OVA-RSV mice. Numbers of neutrophils and ILC3 were also reduced with anti-IL-33 treatment in both RSV and OVA-RSV treated animals as well. Conclusions: Taken together, our findings indicate a broad reduction in allergic-proinflammatory events mediated by IL-33 in RSV-induced asthma exacerbation.

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“…The unprotected exposure to organic dust, which severely damages the respiratory system [48][49][50], is frequent in most cotton ginning operations in small agro-industries of developing countries in the 21st century. In this operation, the roller mills [51] are still used, operating similarly to the ones of 19th-century cotton farms.…”

Section: Occupational Hygiene In Modern Slaverymentioning

confidence: 99%

Identifying Modern Slavery through Occupational Hygiene Conditions of Nineteenth-Century Slaves

Garreto¹,

Baptista²,

Mota³

et al. 2019

Preprint

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The Brazilian economy, the rural in particular, was until the end of the 19th century based on slave labour. In this research, it was intended to obtain, through a review of historical descriptive studies, a detailed picture of the occupational hygiene conditions related to the slaves’ work and their interactions with climatic and environmental conditions. The search was done in the following databases: Science Direct, Scopus, Web of Science, Criminal Justice, Ebsco, Business Source Supplement, as well as original historical documents. Descriptive studies, without the restriction of language, were selected that involved the rural work of slaves in colonial and imperial Brazil. Working environmental conditions have been evaluated: environmental and occupational hygiene conditions to which the captive workers were exposed, as well as their accommodation and clothing. The analysed studies registered the existence of accommodation and similar dresses all over Brazil, regardless of the region's climate. In addition to these accommodation conditions, slaves were still exposed in a similar way to physical, chemical and biological agents throughout the country. Finally, it was also possible to identify a clear similarity with the occupational exposure conditions of the modern slaves in the 21st century.

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Post-Injury and Resolution Response to Repetitive Inhalation Exposure to Agricultural Organic Dust in Mice

Cited by 19 publications

References 49 publications

Ovalbumin-sensitized mice have altered airway inflammation to agriculture organic dust

Ovalbumin-sensitized mice have altered airway inflammation to agriculture organic dust

Neutralization of IL-33 Modifies the Type 2 and Type 3 Inflammatory Signature of Viral Induced Asthma Exacerbation.

Identifying Modern Slavery through Occupational Hygiene Conditions of Nineteenth-Century Slaves

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