Post-Irradiation Changes in Sertoli Cells

Kochar, Nirmal K.; Bateman, A. J.

doi:10.1530/jrf.0.0180265

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…Earlier studies found Sertoli cell death following irradiation, but these were all studies that used higher irradiation doses [45,46]. SMA staining of PTM cells did not suggest any histological changes in the PTM cells.…”

Section: Discussionmentioning

confidence: 76%

Transcriptome profiling of mice testes following low dose irradiation

Belling

Tanaka

Dalgaard

et al. 2013

Reprod Biol Endocrinol

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BackgroundRadiotherapy is used routinely to treat testicular cancer. Testicular cells vary in radio-sensitivity and the aim of this study was to investigate cellular and molecular changes caused by low dose irradiation of mice testis and to identify transcripts from different cell types in the adult testis.MethodsTranscriptome profiling was performed on total RNA from testes sampled at various time points (n = 17) after 1 Gy of irradiation. Transcripts displaying large overall expression changes during the time series, but small expression changes between neighbouring time points were selected for further analysis. These transcripts were separated into clusters and their cellular origin was determined. Immunohistochemistry and in silico quantification was further used to study cellular changes post-irradiation (pi).ResultsWe identified a subset of transcripts (n = 988) where changes in expression pi can be explained by changes in cellularity. We separated the transcripts into five unique clusters that we associated with spermatogonia, spermatocytes, early spermatids, late spermatids and somatic cells, respectively. Transcripts in the somatic cell cluster showed large changes in expression pi, mainly caused by changes in cellularity. Further investigations revealed that the low dose irradiation seemed to cause Leydig cell hyperplasia, which contributed to the detected expression changes in the somatic cell cluster.ConclusionsThe five clusters represent gene expression in distinct cell types of the adult testis. We observed large expression changes in the somatic cell profile, which mainly could be attributed to changes in cellularity, but hyperplasia of Leydig cells may also play a role. We speculate that the possible hyperplasia may be caused by lower testosterone production and inadequate inhibin signalling due to missing germ cells.

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Section: Discussionmentioning

confidence: 76%

Transcriptome profiling of mice testes following low dose irradiation

Belling

Tanaka

Dalgaard

et al. 2013

Reprod Biol Endocrinol

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“…Neonatal [47] or pubertal exposure to a single dose of γ-ray did not result in significantly reduced testis weight, except for testes collected from 60 d/o mice irradiated at 25 day old (Table 2). Other studies have reported reduced testes weights after irradiation of fetal [48,49], pubertal [50] and adult mice [51,52], with IR doses ranging from 0.25 Gy to 6.0 Gy. The different mouse stains (C57BL/6 in the present study vs. CBA/P [52]), animal ages (prepuberty vs. fetal [48,49] or adult [51,52]) could partly explain the discrepancy.…”

Section: Discussionmentioning

confidence: 98%

Recovery of a low mutant frequency after ionizing radiation-induced mutagenesis during spermatogenesis

Intano

McCarrey

et al. 2008

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Humans are exposed to ionizing radiation (IR) under various circumstances, e.g. cosmic radiation, diagnostic X-rays and radiotherapy for cancer. It has been shown that IR can impair spermatogenesis and can cause mutations in germ cells. However, the mutagenic responses of germ cells exposed to IR at different stages of testicular maturation have not been examined by directly assessing the mutant frequency in defined spermatogenic cell types. This study was performed to address whether preadult exposure to IR can increase mutations in adult germ cells that could in turn have a major impact on adult reproductive function and the health of ensuing offspring. Male Lac I transgenic mice were irradiated with a single dose of 2.5 Gy of γ-ray at different ages before adulthood, reflecting different stages of testicular maturation, and then mutant frequency (MF) was determined directly in spermatogenic cell types emanating from the irradiated precursor cells. The results showed that (1) preadult exposure to IR did not significantly increase MF in adult epididymal spermatozoa; (2) spermatogenic stages immediately following the irradiated stage(s) displayed an elevated mutant frequency, but (3) the mutant frequency was restored to unirradiated levels in later stages of spermatogenesis. These findings provide evidence that there is a mechanism(s) to prevent spermatogenic cells with elevated mutant frequencies from progressing through spermatogenesis.

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“…The effect of X-rays on the germinal epithelium of the testis has been observed by numerous investigators (Bergonié & Tribondeau, 1906; Kivy, 1951;Shaver, 1953;Oakberg, 1955a, b;Bateman, 1956;Leidl, 1964;Mandi, 1964;Kochar & Bateman, 1969), but their effect on the interstitial tissue and its production of steroid hormones is limited.…”

Section: Introductionmentioning

confidence: 94%

The Effect of X-Rays on Lipids, Phospholipids and Cholesterol of the Mouse Testis

Kochar¹,

Harrison²

1971

Reproduction

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Summary. The testes of mature male A2G mice were exposed to a single dose of 1000 r of X-rays, and examined at varying intervals up to 146 days after irradiation. Lipids, phospholipids and cholesterol were studied in the control and irradiated mice using histochemical techniques, and estimated quantitatively by the paper weight technique.The relative proportion of lipids per unit area of testis decreased 2 and 6 days after exposure, but increased dramatically from 20 to 72 days; by 146 days, it had nearly returned to the normal level.The relative proportion per unit area of tissue containing phospholipid decreased 2 and 10 days after exposure, steadily rose at 12 days and after until 103 days, and returned to normal at 146 days. The relative proportion of cholesterol increased markedly after 20 days but at 103 and 146 days, it fell to the control level. The significance of these findings is discussed.

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Post-Irradiation Changes in Sertoli Cells

Cited by 6 publications

References 9 publications

Transcriptome profiling of mice testes following low dose irradiation

Transcriptome profiling of mice testes following low dose irradiation

Recovery of a low mutant frequency after ionizing radiation-induced mutagenesis during spermatogenesis

The Effect of X-Rays on Lipids, Phospholipids and Cholesterol of the Mouse Testis

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