Post-ischemic Infusion of Atrial Natriuretic Peptide Attenuates Warm Ischemia–Reperfusion Injury in Rat Lung

Aoyama, Akihiro; Chen, Fengshi; Fujinaga, Takuji; Sato, Susumu; Tsuruyama, Tatsuaki; Zhang, Jitian; Tanaka, Shōji; Sakai, Hiroaki; Nakamura, Takayuki; Date, Hiroshi; Wada, Hiromi; Bando, Toru

doi:10.1016/j.healun.2009.03.015

Cited by 14 publications

(8 citation statements)

References 47 publications

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“…All the lungs were reperfused successfully in 80 min. Thus, consistent with previous reports [12–17], our isolated rat lung perfusion model remained stable throughout the observation period.…”

Section: Resultssupporting

confidence: 91%

Therapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model

Ohsumi¹,

Chen²,

Nakajima³

et al. 2012

Transplant International

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Summary Warm ischemia‐reperfusion injury related to donation after cardiac death donors is a crucial and inevitable issue. As surfactant function is known to deteriorate during warm ischemia, we hypothesized that surfactant inhalation during warm ischemia would mitigate warm ischemia‐reperfusion injury. We used an isolated rat lung perfusion model. The rats were divided into three groups: sham, control, and surfactant. In the control and surfactant groups, cardiac arrest was induced by ventricular fibrillation. Ventilation was restarted 110 min later; subsequently, the lungs were flushed, and heart and lung block was recovered. In the surfactant group, a natural bovine surfactant Surfacten® was inhaled for 3 min at the end of warm ischemia. Then, the lungs were reperfused for 80 min. Surfactant inhalation significantly improved graft functions, effectively increased lung tissue ATP levels, and significantly decreased mRNA levels of IL‐6 and IL‐6/IL‐10 ratio at the end of reperfusion. Histologically, lungs in the surfactant group showed fewer signs of interstitial edema and hemorrhage, and significantly less neutrophilic infiltration than those in the control group. Our results indicated that surfactant inhalation in the last phase of warm ischemia maintained lung tissue energy levels and prevented cytokine production, resulting in the alleviation of warm ischemia‐reperfusion injury.

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Section: Resultssupporting

confidence: 91%

Therapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model

Ohsumi¹,

Chen²,

Nakajima³

et al. 2012

Transplant International

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“…Studies have shown that ANP can not only protect the myocardium from damage caused by IRI (Kousholt, 2012) but can also have a protective effect on the lungs and liver. The mechanism of the protective effect of ANP is related to reduction in oxidative stress, inhibition of inflammation and inflammatory cell infiltration, and protection of endothelial function (Dodd-o et al, 2008;Aoyama et al, 2009;Yamada et al, 2013). A recent study on renal IRI reported that ANP also plays a protective role in ischemic renal injury (Koga et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of lyophilized recombinant human brain natriuretic peptide on renal ischemia/reperfusion injury in mice

et al. 2015

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ABSTRACT. Brain natriuretic peptide (BNP) has a protective effect on acute injury of the heart, brain, and lung. However, its role in acute kidney injury (AKI) remains unclear. The aim of this study was to investigate the effect of lyophilized recombinant human BNP (lrh-BNP) on AKI and the underlying molecular mechanisms. An experimental model for AKI was established using an ischemia/reperfusion (I/R) procedure. Healthy adult BALB/c mice were randomized to the sham, I/R, and lrh-BNP-treated post-I/R (BNP + I/R) groups. Post-operatively, the BNP + I/R group was subcutaneously injected with lrh-BNP (0.03 µg·kg -1 ·min -1 ), whereas the other groups received saline at the same dose. Serum creatinine (Scr) and blood urea nitrogen levels were examined; tissue staining was performed to evaluate the degree of I/R injury (IRI). Ki67 positive staining of renal tubular epithelial cells was observed using immunofluorescence confocal laser scanning to assess the effect of BNP on cell proliferation after IRI. Inflammatory factor expression levels were detected to evaluate the effect of BNP on renal inflammation. Compared with the sham group, the I/R 13301 Effect of BNP on renal IRI ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 13300-13311 (2015) group showed increased Scr levels, severe tubular injury of the renal outer medulla, increased Kim-1 mRNA expression, an increased number of infiltrative macrophages in the renal interstitium, and increased TNF-α, IL-1β, IL-6, MCP-1, and HIF-1α mRNA expression. BNP delivery significantly reduced all pathological changes in the I/R group. The protective role of BNP in murine renal IRI may be associated with its inhibition of renal interstitial inflammation and hypoxia and its promotion of renal tubule repair.

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“…[19] revealed that administration of ANP infusion at the onset of lung reperfusion attenuates reperfusion injury.…”

Section: Discussionmentioning

confidence: 99%

The effect of atrial natriuretic peptide infusion on intestinal injury in septic shock

Elbaradey

Elshmaa

Hodeib

2016

J Anaesthesiol Clin Pharmacol

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Background and Aims:The aim of this study is to assess the effect of atrial natriuretic peptide (ANP) on intestinal ischemia-reperfusion injury in septic shock.Material and Methods:A prospective randomized controlled, observer-blinded study was carried out in surgical Intensive Care Unit (ICU), University Hospital. Forty adult patients in septic shock were randomly divided into two groups, control group (Group C) received normal saline and ANP group (Group A) patients received ANP in the form of 1.5 mg vial added to 250 ml solvent in plastic bag (1 ml = 6 micg) given at 2 mcg/kg intravenous bolus over 1 min followed by 0.01 mcg/kg/min for 24 h. The primary outcome measurements were blood marker of intestinal hypoperfusion in form of intestinal fatty acid binding protein (I-FABP), malondialdehyde (MDA), myloperoxidase enzyme activity (MPO), protein carbonyl (PC), and glutathione peroxidase activity (GPA) measured before start of ANP infusion, 6 h, 12 h, and 24 h after start of infusion. The secondary outcome measurements were the duration of noradrenaline infusion, duration of ICU stay, hospital mortality rate, and complications related to ANP.Results:In comparison with Group C, Group A showed a significant decrease (P < 0.05) in serum level of MPO, MDA, PC, and I-FABP, with a significant increase (P < 0.05) in serum level of GPA, 6 h, 12 h, and 24 h after the start of ANP infusion. There was significant decrease (P < 0.05) in mean duration of noradrenaline infusion, the length of ICU stay and mortality rate in Group A in comparison with Group C. In Group A, seven patients had mean arterial blood pressure < 65 mmHg but respond to volume resuscitation, three patients serum sodium was 125–130 mmol/L.Conclusion:In cases of septic shock, concomitant administration of ANP with noradrenaline may have a protective effect against intestinal injury through a decrease in the level of intestinal hypoperfusion owing to its anti-inflammatory and antioxidant effect.

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Post-ischemic Infusion of Atrial Natriuretic Peptide Attenuates Warm Ischemia–Reperfusion Injury in Rat Lung

Abstract: Our results indicate that ANP administered at the onset of reperfusion increases cGMP in lung tissue and attenuates warm ischemia-reperfusion injury in isolated perfused rat lung.

Cited by 14 publications

References 47 publications

Therapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model

Therapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model

Protective effect of lyophilized recombinant human brain natriuretic peptide on renal ischemia/reperfusion injury in mice

The effect of atrial natriuretic peptide infusion on intestinal injury in septic shock

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