Post-mating effects in the grasshopper, Gomphocerus rufus L. mediated by the spermatheca

Hartmann, Rüdiger; Loher, Werner

doi:10.1007/s003590050330

Cited by 45 publications

(39 citation statements)

References 20 publications

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“…The present immunoassays on virgin female tissue extracts of H. armigera revealed a weak non-spe- The permeable nature of the female Bu-St, enabling the translocation of seminal peptides to female HL has been highlighted in many insect species (Chen, 1984;Hartmann and Loher, 1999;Gillott, 2003;Lay et al, 2004). The processing of seminal peptides in the female genital tract for the transfer into the circulatory system has also been demonstrated in insect species such as locust (Lay et al, 2004) and D. melanogaster Ram et al, 2005).…”

Section: Drmsp-ir In Female Tissuesmentioning

confidence: 70%

Female sex pheromone suppression and the fate of sex‐peptide‐like peptides in mated moths of Helicoverpa armigera

Nagalakshmi

Applebaum

Azrielli

et al. 2007

Arch Insect Biochem Physiol

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Insect males produce accessory gland (MAG) factors that are transferred in the seminal fluid to females during copulation, and elicit changes in the mated female's behavior and physiology. Our previous studies showed that the injection of synthetic Drosophila melanogaster sex-peptide (DrmSP) into virgin females of the moth Helicoverpa armigera causes a significant inhibition of pheromone production. In this and other moth species, pheromone production, correlated with female receptivity, is under neuroendocrine control due to the circadian release of the neuropeptide PBAN. In this study, we show that PBAN, present in the hemolymph during the scotophase in females, is drastically reduced after mating. We also identify 4 DrmSP-like HPLC peaks (Peaks A, S1, S2, and B) in MAGs, with increasing levels of DrmSP immunoreactivity during the scotophase, when compared to their levels observed during the photophase. In H. armigera MAGs, a significant reduction in the pheromonostatic peak (Peak B) was already evident after 15 min of copulation, and depletion of an additional peak (Peak S2) was evident after complete mating. Peak A is also detected in female brains, increasing significantly 1 h after mating, at which time inhibition of pheromone biosynthesis also occurs. However, changes corresponding to the other MAG peaks were not detected in mated female tissues.

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Section: Drmsp-ir In Female Tissuesmentioning

confidence: 70%

Female sex pheromone suppression and the fate of sex‐peptide‐like peptides in mated moths of Helicoverpa armigera

Nagalakshmi

Applebaum

Azrielli

et al. 2007

Arch Insect Biochem Physiol

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“…An unequivocal answer brought the method of artificial secretion transfer. Small amounts of radioactive WS-secretions were injected under hydraulic pressure into the spermatheca with a plastic tube, whose tip fitted the entrance to the spermathecal duct (Hartmann and Loher, 1999). The results proved to be identical to those obtained through WS transfer during insemination.…”

Section: Ws In the Hemocoelmentioning

confidence: 76%

“…Thus, epithelial gland cells transport substances in both directions. In the locust and in Gomphocerus rufus (Hartmann and Loher 1999), the main pathway for the transport of resorptive material such as the male's insoluble or partially soluble WS is indicated by strong antibody affinity of the coarse secretory granules. They were traced from the spermathecal lumen into and along the cuticular channels, which connect the lumen with the extracellular cavities of the gland cells.…”

Section: Discussionmentioning

confidence: 99%

“…Daily injections of 3 H amino acids amounted to 2 ´ 15 mCi (one injection of 15 mCi every 12 h for 5 days), and for 14 C amino acids, the daily amount was 2 ´ 3.75 mCi (one injection of 3.75 mCi every 12 h for 6 days). Radioactive WS were transferred to the spermatheca via a spermatophore or by artificial transfer described in detail by Hartmann and Loher (1999).…”

Section: Isotope Technologymentioning

confidence: 99%

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Pathways and destination of some male gland secretions in female Locusta migratoria migratorioides (R&F) after insemination

Lay¹,

Loher²,

Hartmann³

2003

Arch Insect Biochem Physiol

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The white secretions (WS) from the tubules of the male accessory glands (AG) of Locusta migratoria are composed of peptides and proteins. The WS are transferred during mating to the female's spermatheca. They have been followed to their destinations with immunological and radioactive marker techniques. In the spermatheca, peptides are split off from WS-protein complexes, permeate the spermathecal epithelium via glandular cells, enter the hemolymph and attach to other proteins in various target organs such as the dorsal fat body, the preterminal/terminal oocytes, and the follicular cells. In developing eggs, they concentrate at the posterior pole where sperm enters the egg, and in early embryogenesis they are found in the germ band. These results extend the functions of the spermatheca and the role of the male during the reproductive process.

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“…65 In this insect spermathecal gland cells digest and resorb seminal secretions, and the neural pathway between the spermatheca and the ventral nerve cord is required to maintain sexual refractoriness. 66 Recently, it has been shown in Drosophila that spermathecal secretory cells attract sperm into the female sperm storage organ and participate in modulating sperm motility and stimulating oviposition. 67 A possible role of spermathecal cells in Anopheles post-mating behavior needs to be fully established.…”

Section: Sperm Effect In Female Post-mating Responsesmentioning

confidence: 99%

Function and composition of male accessory gland secretions inAnopheles gambiae: a comparison with other insect vectors of infectious diseases

Baldini

Gabrieli

Rogers

et al. 2012

Pathogens and Global Health

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Post-mating effects in the grasshopper, Gomphocerus rufus L. mediated by the spermatheca

Cited by 45 publications

References 20 publications

Female sex pheromone suppression and the fate of sex‐peptide‐like peptides in mated moths of Helicoverpa armigera

Female sex pheromone suppression and the fate of sex‐peptide‐like peptides in mated moths of Helicoverpa armigera

Pathways and destination of some male gland secretions in female Locusta migratoria migratorioides (R&F) after insemination

Function and composition of male accessory gland secretions inAnopheles gambiae: a comparison with other insect vectors of infectious diseases

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