Post-modified porous hollow nanospheres incorporating multiple strategies for comprehensive phosphoproteomics analysis of serum of Alzheimer's disease

Du, Juan; Tian, Haihua; Meng-Yao, FU; Yan, Yinghua; Wang, Chuang; Ding, Chuan‐Fan

doi:10.1016/j.micromeso.2022.112066

Cited by 8 publications

(5 citation statements)

References 41 publications

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“…Reproduced with permission. [90] Copyright 2022, Elsevier. C) The analysis of proteins in lung cancer personalized coronas formed on Gd@C 82 (OH) 22 NPs to discover potential biomarkers for disease diagnosis.…”

Section: Specific Enrichment Of Proteinmentioning

confidence: 99%

“…Ultimately, the platform identified multiple phosphoproteins and phosphopeptides in serum and revealed several peptides and proteins correlated with Alzheimer's disease. [90] Glycoproteins are another essential and most structurally complex protein modification that profoundly affects numerous biological and pathological processes. [114] The aberrant glycosylation of proteins has been proven to be associated with many human diseases, such as blood disorders, neurodegenerative diseases, and even cancer.…”

Section: Specific Enrichment Of Proteinmentioning

confidence: 99%

“…Ultimately, the platform identified multiple phosphoproteins and phosphopeptides in serum and revealed several peptides and proteins correlated with Alzheimer's disease. [ 90 ]…”

Section: Application Of Proteomics and Metabolomics Analysis For Ivdmentioning

confidence: 99%

mentioning

confidence: 99%

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Designed Nanomaterials‐Assisted Proteomics and Metabolomics Analysis for In Vitro Diagnosis

Wang,

Li,

Shu

et al. 2023

Small Methods

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In vitro diagnosis (IVD) is pivotal in modern medicine, enabling early disease detection and treatment optimization. Omics technologies, particularly proteomics and metabolomics, offer profound insights into IVD. Despite its significance, omics analyses for IVD face challenges, including low analyte concentrations and the complexity of biological environments. In addition, the direct omics analysis by mass spectrometry (MS) is often hampered by issues like large sample volume requirements and poor ionization efficiency. Through manipulating their size, surface charge, and functionalization, as well as the nanoparticle‐fluid incubation conditions, nanomaterials have emerged as a promising solution to extract biomolecules and enhance the desorption/ionization efficiency in MS detection. This review delves into the last five years of nanomaterial applications in omics, focusing on their role in the enrichment, separation, and ionization analysis of proteins and metabolites for IVD. It aims to provide a comprehensive update on nanomaterial design and application in omics, highlighting their potential to revolutionize IVD.

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Section: Specific Enrichment Of Proteinmentioning

confidence: 99%

Section: Specific Enrichment Of Proteinmentioning

confidence: 99%

“…Ultimately, the platform identified multiple phosphoproteins and phosphopeptides in serum and revealed several peptides and proteins correlated with Alzheimer's disease. [ 90 ]…”

Section: Application Of Proteomics and Metabolomics Analysis For Ivdmentioning

confidence: 99%

mentioning

confidence: 99%

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Designed Nanomaterials‐Assisted Proteomics and Metabolomics Analysis for In Vitro Diagnosis

Wang,

Li,

Shu

et al. 2023

Small Methods

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“…ulation of phosphorylation may result in some serious diseases such as degenerative diseases and cancers [5][6][7][8][9]. For example, one of the biomarkers of Alzheimer's disease is the highly heterogeneous phosphorylation of Tau protein [10][11][12]. Therefore, systematic and in-depth study of protein phosphorylation contributes to understanding the pathogenesis of diseases.…”

Section: Introductionmentioning

confidence: 99%

Combination of click chemistry and Schiff base reaction: Post‐synthesis of covalent organic frameworks as an immobilized metal ion affinity chromatography platform for efficient capture of global phosphopeptides in serum with chronic obstructive pulmonary disease

Xu,

Guo,

Wang

et al. 2024

J of Separation Science

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Reasonable design and construction of functionalized materials are of great importance for the enrichment of global phosphopeptides. In this work, Ti4+ functionalized hydrophilic covalent organic frameworks by introducing glutathione (GSH) and 2,3,4‐trihydroxy benzaldehyde (THBA) via click chemistry and Schiff base reaction (COF‐V@GSH‐THBA‐Ti4+) was constructed and applied for selective enrichment of phosphopeptides in serum. Benefit from the high surface area, excellent hydrophilicity as well as regular mesoporous structure, COF‐V@GSH‐THBA‐Ti4+ displayed high selectivity (molar ratio of 2000:1), low limit of detection (0.5 fmol), high load capacity (100.0 mg/g) and excellent size‐exclusion effect (1:10000) for enrichment of phosphopeptides. For actual bio‐sample analysis, 15 phosphopeptides assigned to 10 phosphoproteins with 16 phosphorylated sites and 33 phosphopeptides assigned to 25 phosphoproteins with 34 phosphorylated sites were detected from the serum of patients with chronic obstructive pulmonary disease (COPD), and normal controls. Biological processes and molecular functions analysis further disclosed the difference of serums with phosphoproteomics between COPD and normal controls.

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Ti4+ functionalized zirconium metal-organic frameworks with polymer brushes for specific identification of phosphopeptides in human serum and skimmed milk

Chen,

Wang,

Wang

et al. 2024

Anal Bioanal Chem

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Post-modified porous hollow nanospheres incorporating multiple strategies for comprehensive phosphoproteomics analysis of serum of Alzheimer's disease

Cited by 8 publications

References 41 publications

Designed Nanomaterials‐Assisted Proteomics and Metabolomics Analysis for In Vitro Diagnosis

Designed Nanomaterials‐Assisted Proteomics and Metabolomics Analysis for In Vitro Diagnosis

Combination of click chemistry and Schiff base reaction: Post‐synthesis of covalent organic frameworks as an immobilized metal ion affinity chromatography platform for efficient capture of global phosphopeptides in serum with chronic obstructive pulmonary disease

Ti4+ functionalized zirconium metal-organic frameworks with polymer brushes for specific identification of phosphopeptides in human serum and skimmed milk

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