Post-Thaw Viability of European Bison (Bison Bonasus) Semen Frozen With Extenders Containing Egg Yolk or Lipids of Plant Origin and Examined With a Heterologous in Vitro Fertilization Assay

Pérez-Garnelo, S. S.; Oter, M.; Borque, C.; Talavera, C.; Delclaux, M.; Martínez‐Nevado, Eva; Palasz, A.T.; Fuente, J. de la

doi:10.1638/05-039.1

Cited by 18 publications

(19 citation statements)

References 30 publications

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“…Moreover, in both cases, parameters of embryonic development were consistent with the traditional values resulting from IVF in cattle [13,26]. Our findings and the results of other studies [10] suggest that wisent male gametes are capable of recognizing specific carbohydrate sequence of bovine oocyte pellucid zone glycoprotein and overcome the species barrier, and the acrosome enzymes contribute to sperm penetration into xenogenous oocytes. In addition, the fact of two-cell hybrid embryos formation and their development to the blastocyst stage indicates the similarity in the mechanisms of oocyte activation and embryonic development in cow and wisent females.…”

Section: Extracorporeal Fertilization Of Cow (Bossupporting

confidence: 89%

“…In an earlier study, heterogeneous fertilization of bovine oocytes was used to assess the European bison sperm fertility. The effectiveness of insemination was determined by the penetration of sperm through the oocyte PZ and pronuclei formation [10]. However, embryo formation and development is the best evidence of successful gamete interaction.…”

Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

“…In publications, there is evidence of their in vitro fertilization using semen of gaur [5], antelope [6], horse [7], sheep [8], and donkey [9]. Сlose relationship between the species, the lack of specific antigens against wisent spermatozoa in the pellucid zone (PZ) of cow oocytes [10], and the previously described possibility to obtain a hybrid offspring by crossing [11] are the prerequisites for extracorpo- …”

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confidence: 99%

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IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

Сингина¹,

Багиров²,

Danch³

et al. 2016

S-h. biol.

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A b s t r a c t European bison or wisent (Bison bonasus)is a rare species that is endangered. The technology for producing in vitro embryos using oocytes of cows and wisent semen can be an effective tool for conservation of genetic resources of this species and their rational use in basic research on the physiology of development and to create the new animals' types. However, the methods for production of in vitro embryos using the above-mentioned germ cells are currently not described in the literature. In this work, we first attempted to obtain the hybrid cow-wisent embryos in vitro using the IVM/IVF/IVC protocol, developed for domestic cattle (Bos taurus). Frozen epididymal sperm derived from wisent was thawed and prepared by the swim-up procedure. Matured oocytes were coincubated during 18 h together with prepared sperm in Fert-Talp medium, which was replaced then by embrional medium where the embryos were cultured until the blastocyst state. Additionally, for positive control, a part of matured bovine oocytes were fertilized with frozen/thawed ejaculated bull sperm. Fertilizing capacity of male cells of both species was assessed according to the nature of spermatozoa-egg interactions, as well as the capacity of fertilized bovine oocytes to subsequent embryonic development. It was found that the rate of oocytes with sing polyspermy was higher for fertilization with wisent sperm comparing to allogenic insemination (21.6 vs. 8.5 %, P < 0.05). There were no differences between two types of fertilizations nether for the sperm penetration rate (90.0±0.3 vs. 93.3±1.7 % for bull and wisent, respectively), nor for the rate of normal fertilization (78.3±1.7 % vs. 73.2±2.3 %). The similar cleavage and blastocyst formation rates were observed for two fertilization types. Our data indicate the similarity in the mechanisms of oocyte activation and embryonic development in cow and wisent females. In addition, it is obvious that IVM/IVF/IVP protocol, in general, allows in vitro producing cattle-wisent hybrid embryos, however, high levels of polyspermy observed in heterogeneous fertilization, indicates the necessity to adjust the method.Keywords: cattle oocytes, the European bison sperm, fertilization in vitro, hybrid embryos European bison, or wisent, (Bison bonasus) is a rare endangered species. Current European bison population is strongly inbred. All modern wisents come from 12 individuals kept in the zoos and reserves in the early XX century [1]. Low genetic variability is one of the main threats to the long-term maintenance of the species. Assisted reproductive technology such as production of in vitro embryos using oocytes of domestic cattle and wisent semen will not only help to maintain the existing genetic resources of the species, but also to involve this species in basic research on the physiology of development and use in creations of new bred forms [2][3][4].Cow oocytes are a universal object for interspecific hybridization. In publications, there is evidence of their in vitro fertilization using semen of gaur...

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Section: Extracorporeal Fertilization Of Cow (Bossupporting

confidence: 89%

Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

mentioning

confidence: 99%

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IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

Сингина¹,

Багиров²,

Danch³

et al. 2016

S-h. biol.

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“…Similar developments are being observed with the wisent (Pérez‐Garnelo et al. ). Cell culture and banking in combination with SCNT has been gaining acceptance as an alternative for embryo production and genetic preservation for non‐domestic and endangered species (Holt et al.…”

Section: Introductionsupporting

confidence: 85%

In Vitro Development of Bison Embryos Using Interspecies Somatic Cell Nuclear Transfer

Alexander

et al. 2013

Reprod Domestic Animals

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Interspecies somatic cell nuclear transfer (interspecies SCNT) has been explored in many domestic and non-domestic animal species. However, problems arise during the development of these embryos, which may be related to species-specific differences in nuclear-cytoplasmic communication. The objectives of this study were to investigate the possibility of producing bison embryos in vitro using interspecies SCNT and assess the developmental potential of these embryos. Treatment groups consisted of cattle in vitro fertilization (IVF) and cattle SCNT as controls and wood bison SCNT, plains bison SCNT and wisent SCNT as experimental groups. Cleavage and blastocyst rates were assessed, and blastocyst quality was determined using total cell number, apoptotic incidence and relative quantification of mitochondria-related genes NRF1, MT-CYB and TFAM. These results indicate that embryos can be produced by interspecies SCNT in all bison species/subspecies (13.34-33.54% blastocyst rates). Although increased incidence of apoptosis was observed in bison SCNT blastocysts compared to cattle SCNT controls (10.45-12.69 vs 8.76, respectively) that corresponded with significantly lower cell numbers (80-87 cells vs >100 cells, respectively), no major differences were observed in the expression of NRF1, MT-CYB and TFAM. This study is the first to report the production of bison embryos by interspecies SCNT. Blastocyst development in all three bison species/subspecies was greater than the rates obtained in previous studies by IVF, which supports the potential role of SCNT for in vitro embryo production in this species. Yet, further investigation of developmental competence and the factors influencing blastocyst quality and viability is required.

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“…LP1 ℗ lipid vesicles‐based extender and HSPM extender have been successfully used in the European Bison and in the bull species (Pérez‐Garnelo et al. ; Szczesniak‐Fabianczyk et al. ; Tamargo et al.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cryopreservation on the Sperm DNA Fragmentation Dynamics of the Bottlenose Dolphin (Tursiops truncatus)

Sánchez‐Calabuig

López‐Fernández

Johnston

et al. 2015

Reprod Domestic Animals

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Sperm DNA fragmentation is one of the major causes of infertility; the sperm chromatin dispersion test (SCDt) evaluates this parameter and offers the advantage of species-specific validated protocol and ease of use under field conditions. The main purpose of this study was to evaluate sperm DNA fragmentation dynamics in both fresh and post-thaw bottlenose dolphin sperm using the SCDt following different cryopreservation protocols to gain new information about the post-thaw differential sperm DNA longevity in this species. Fresh and cryopreserved semen samples from five bottlenose dolphins were examined for sperm DNA fragmentation dynamics using the SCDt (Halomax(®)). Sperm DNA fragmentation was assessed immediately at collection and following cryopreservation (T0) and then after 0.5, 1, 4, 8, 24, 48 and 72 h incubation at 37°C. Serially collected ejaculates from four dolphins were frozen using different cryopreservation protocols in a TES-TRIS-fructose buffer (TTF), an egg-yolk-free vegetable lipid LP1 buffer (LP1) and human sperm preservation medium (HSPM). Fresh ejaculated spermatozoa initially showed low levels of DNA fragmentation for up to 48 h. Lower Sperm DNA fragmentation (SDF) was found in the second fresh ejaculate compared to the first when more than one sample was collected on the same day (p < 0.05); this difference was not apparent in any other seminal characteristic. While there was no difference observed in SDF between fresh and frozen-thawed sperm using the different cryopreservation protocols immediately after thawing (T0), frozen-thawed spermatozoa incubated at 37°C showed an increase in the rate of SDF after 24 h. Sperm frozen in the LP1(℗) buffer had higher levels (p < 0.05) of DNA fragmentation after 24- and 48-h incubation than those frozen in TTF or HSPM. No correlation was found between any seminal characteristic and DNA fragmentation in either fresh and/or frozen-thawed samples.

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Post-Thaw Viability of European Bison (Bison Bonasus) Semen Frozen With Extenders Containing Egg Yolk or Lipids of Plant Origin and Examined With a Heterologous in Vitro Fertilization Assay

Cited by 18 publications

References 30 publications

IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

In Vitro Development of Bison Embryos Using Interspecies Somatic Cell Nuclear Transfer

Effect of Cryopreservation on the Sperm DNA Fragmentation Dynamics of the Bottlenose Dolphin (Tursiops truncatus)

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