Post-transcriptional control of virulence gene expression in Staphylococcus aureus

Scornet, Alexandre Le; Redder, Peter

doi:10.1016/j.bbagrm.2018.04.004

Cited by 7 publications

(5 citation statements)

References 64 publications

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“…Previous studies demonstrate that RNase Y directly and indirectly impacts sRNA levels in several important Gram-positive pathogens ( 25 , 36 , 37 ), whereas PNPase promotes the stability of some sRNAs and degrade others in E. coli ( 38 , 39 ). A recent Grad-seq study indicates that S. pneumoniae PNPase binds to several sRNAs, including CcnA, CcnB, CcnC, CcnD, and Spd_sr34 ( 23 ).…”

Section: Resultsmentioning

confidence: 99%

Pivotal Roles for Ribonucleases in Streptococcus pneumoniae Pathogenesis

Sinha

Frick

Clemons

et al. 2021

mBio

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Section: Resultsmentioning

confidence: 99%

Pivotal Roles for Ribonucleases in Streptococcus pneumoniae Pathogenesis

Sinha

Frick

Clemons

et al. 2021

mBio

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“…RNase Y and PNPase mediate the sRNA transcriptome of S. pneumoniae. Previous studies demonstrate that RNase Y directly and indirectly impacts sRNA levels in several important Gram-positive pathogens (25,36,37), whereas, PNPase promotes the stability of some sRNAs and degrade others in E. coli (38,39). A recent Grad-seq study indicates that S. pneumoniae PNPase binds to several sRNAs, including CcnA, CcnB, CcnC, CcnD, and Spd_sr34 (23).…”

Section: Resultsmentioning

confidence: 99%

Pivotal Roles for Ribonucleases inStreptococcus pneumoniaePathogenesis

Sinha

Frick

Clemmons

et al. 2021

Preprint

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RNases perform indispensable functions in regulating gene expression in many bacterial pathogens by processing and/or degrading RNAs. Despite the pivotal role of RNases in regulating bacterial virulence factors, the functions of RNases have not yet been studied in the major human respiratory pathogen Streptococcus pneumoniae (pneumococcus). Here, we sought to determine the impact of two conserved RNases, the endoribonuclease RNase Y and exoribonuclease polynucleotide phosphorylase (PNPase), on the physiology and virulence of S. pneumoniae serotype 2 strain D39. We report that RNase Y and PNPase are essential for pneumococcal pathogenesis as both deletion mutants showed strong attenuation of virulence in murine models of invasive pneumonia. Genome-wide transcriptomic analysis revealed that nearly 200 mRNA transcripts were significantly up-regulated, whereas the abundance of several pneumococcal sRNAs, including the Ccn (CiaR Controlled Noncoding RNA) sRNAs, were altered in the Δrny mutant relative to the wild-type strain. Additionally, lack of RNase Y resulted in pleiotropic phenotypes that included defects in pneumococcal cell morphology and growth in vitro. In contrast, Δpnp mutants showed no growth defect in vitro, but differentially expressed a total of 40 transcripts including the tryptophan biosynthesis operon genes and numerous 5’-cis-acting regulatory RNAs, a majority of which were previously shown to impact pneumococcal disease progression in mice using the serotype 4 strain TIGR4. Altogether our data suggest that RNase Y exerts a global impact on pneumococcal physiology, while PNPase-mediates virulence phenotypes, likely through sRNA regulation.IMPORTANCEStreptococcus pneumoniae is a notorious human pathogen that adapts to conditions in distinct host tissues and responds to host cell interactions by adjusting gene expression. Ribonucleases (RNases) are key players that modulate gene expression by mediating the turnover of regulatory and protein-coding transcripts. Here, we characterized two highly conserved RNases, RNase Y and PNPase, and evaluated their impact on the S. pneumoniae transcriptome for the first time. We show that PNPase influences the levels of a narrow set of mRNAs, but a large number of regulatory RNAs primarily implicated in virulence control, whereas RNase Y has a more sweeping effect on gene expression, altering levels of transcripts involved in diverse cellular processes including cell division, metabolism, stress response, and virulence. This study further reveals that RNase Y regulates expression of genes governing competence by mediating the turnover of CiaR-controlled-noncoding (Ccn) sRNAs.

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“…Therefore, a decrease in RNAIII levels in S. aureus was expected to increase the production of Staphylococcus protein A in the culture supernatant; however, in contrast, it tended to decrease compared with the control. The amount of RNAIII in the cell is not unlimited, especially in the middle of the exponential growth phase when the quorum sensing system is not yet fully activated (Le Scornet and Redder, 2019). This suggests that various target mRNAs must compete for binding to RNAIII.…”

Section: Discussionmentioning

confidence: 99%

Slightly acidic electrolyzed water inhibits inflammation induced by membrane vesicles of Staphylococcus aureus

Shimamura,

Oura,

Tsuchiya

et al. 2024

Front. Microbiol.

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Staphylococcus aureus grows in the skin of patients with atopic dermatitis and the associated symptoms are induced by membrane vesicles (MVs). This study explored the effects of slightly acidic electrolyzed water (SAEW) on the expression of virulence factors of S. aureus and MV-induced inflammation to uncover the potential of SAEW as a new treatment method for atopic dermatitis. Expression levels of genes related to virulence factors in S. aureus was assessed and S. aureus-derived MVs were characterized. Moreover, expression level of MV-induced Type I allergic reaction-related genes in RBL2H3 cells was also assessed. Significantly decreased staphylococcal enterotoxin A production and decreased virulence factor-related gene expression were observed after culturing S. aureus in broth supplemented with SAEW at ratios of 1, 2, and 5 per broth. MVs prepared by culturing S. aureus in SAEW-supplemented broth exhibited altered particle size and markedly reduced staphylococcal enterotoxin A content under all addition conditions; moreover, those obtained at a ratio of 1:5 (broth:SAEW) exhibited a reduction in the expression of several proteins associated with hemolytic activity and free iron uptake. The MVs prepared in SAEW-supplemented broth also exhibited remarkably reduced allergy-related gene expression levels in rat cell lines derived from basophilic leukemia-2H3 cells. Overall, SAEW is expected to suppress atopic dermatitis symptoms through the alteration of the properties of S. aureus-derived MVs.

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Post-transcriptional control of virulence gene expression in Staphylococcus aureus

Cited by 7 publications

References 64 publications

Pivotal Roles for Ribonucleases in Streptococcus pneumoniae Pathogenesis

Pivotal Roles for Ribonucleases in Streptococcus pneumoniae Pathogenesis

Pivotal Roles for Ribonucleases inStreptococcus pneumoniaePathogenesis

Slightly acidic electrolyzed water inhibits inflammation induced by membrane vesicles of Staphylococcus aureus

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