Plasmids in Bacteria 1985
DOI: 10.1007/978-1-4613-2447-8_58
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Post-Transcriptional Regulation of Chloramphenicol Acetyl Transferase

Abstract: Volume 155, no. 2, p. 452, column 1, line 10: "labeled W7 cells" should read "labeled E. coli 5509 cells."

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Cited by 16 publications
(20 citation statements)
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“…Vector pJM116 (5) contains the promoterless spoVG-lacZ gene between two fragments of the B. subtilis amyE gene and carries the cat gene from pC194 (4). The integration vector pCM160 was constructed by cloning an 819-bp-long PCR fragment of the citM promoter region, PcitM, into the multiple cloning site of pJM116.…”
Section: Methodsmentioning
confidence: 99%
“…Vector pJM116 (5) contains the promoterless spoVG-lacZ gene between two fragments of the B. subtilis amyE gene and carries the cat gene from pC194 (4). The integration vector pCM160 was constructed by cloning an 819-bp-long PCR fragment of the citM promoter region, PcitM, into the multiple cloning site of pJM116.…”
Section: Methodsmentioning
confidence: 99%
“…The complementation studies were begun assuming that the pTl81 tet region contains two ORFs, as mentioned above. In these studies we used pC194 as a vector for cloning the 3' end of the tet region, since this plasmid is compatible with pT181 and carries a selectable chloramphenicol resistance (Cmr) marker (6). pRN8008 (a high-copy-number mutant of pTl81 containing an intact tetracycline resistance region) (7), was digested with RsaI and HindIll, and a 1,466-bp fragment containing the 3' half of the tet region (Fig.…”
mentioning
confidence: 99%
“…A model for translational attenuation has been proposed for the regulation of the tet genes of pT181, pTHT15, and pNS1981 and for the ermC (ribosomal meth- ylase)-mediated erythromycin resistance encoded by plasmid pE194 (17,18,20,29,37,40). Posttranscriptional regulation has also been hypothesized for chloramphenicol resistance systems (2,6,14).…”
mentioning
confidence: 99%
“…The discrepancy between our results and those' reported previously (2) prompted us to introduce a control by assaying a constitutive mRNA in the extracts. RNA was extracted from strains QB128 and QB136 which were transformed with pLG131 (the integrated plasmid codes for a constitutive cat mRNA [4]). The filters were successively hybridized with probes complementary to sacB mRNA and cat mRNA.…”
mentioning
confidence: 99%