Post-transcriptional regulation of glyceraldehyde-3-phosphate-dehydrogenase gene expression in rat tissues

Piechaczyk, Marc; Blanchard, Jean‐Marie; Marty, L.; Dani, Christian; Panabieres, F.; Sabouty, S. El; Fort, Philippe; Jeanteur, Philippe

doi:10.1093/nar/12.18.6951

Cited by 453 publications

(220 citation statements)

References 32 publications

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“…It seems more probable that regulation was occurring at the translational level or subsequent to it in these tissues, a result in keeping with the preliminary observations of Piechaczyk et al [7] on LDH A mRNA levels in a number of different rat tissues. The nature of this regulatory process remains quite unknown at the present time.…”

Section: Discussionsupporting

confidence: 82%

Rat lactate dehydrogenase A and B subunit concentrations are not regulated by mRNA abundance in liver and heart

Skidmore

Beebee

1990

FEBS Letters

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RNA was isolated from rat liver and heart tissues at various times up to 12 weeks after birth, and probed on slot blots with lactate dehydrogenase A and B cDNA probes. Although the relative abundances of LDH A in liver and LDH B in heart increased substantially in the 12 weeks after birth, mRNAs for both isoenzymes remained remarkably stable in both tissues over the same period. The implications of these observations for the regulation of constitutive gene expression are discussed.

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Section: Discussionsupporting

confidence: 82%

Rat lactate dehydrogenase A and B subunit concentrations are not regulated by mRNA abundance in liver and heart

Skidmore

Beebee

1990

FEBS Letters

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“…As early as 1975, 18S rRNA was reported to increase in expression with cytomegalovirus infection. 12 In 1984, Piechaczyk et al 13 reported that while GAPDH transcription occurred at a similar rate in different rat tissues, they contained very different amounts of mRNA. In 1985, HPRT was reported to be constitutively expressed at low levels in most human tissues but was elevated in certain parts of the central nervous system 14 and in 1987 b-actin mRNA was reported to be differentially expressed in different leukaemia patient tumour samples.…”

Section: Normalisation; Reference Genesmentioning

confidence: 99%

Real-time RT-PCR normalisation; strategies and considerations

et al. 2005

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Real-time RT-PCR has become a common technique, no longer limited to specialist core facilities. It is in many cases the only method for measuring mRNA levels of vivo low copy number targets of interest for which alternative assays either do not exist or lack the required sensitivity. Benefits of this procedure over conventional methods for measuring RNA include its sensitivity, large dynamic range, the potential for high throughout as well as accurate quantification. To achieve this, however, appropriate normalisation strategies are required to control for experimental error introduced during the multistage process required to extract and process the RNA. There are many strategies that can be chosen; these include normalisation to sample size, total RNA and the popular practice of measuring an internal reference or housekeeping gene. However, these methods are frequently applied without appropriate validation. In this review we discuss the relative merits of different normalisation strategies and suggest a method of validation that will enable the measurement of biologically meaningful results.

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“…The filters were exposed overnight using X-Omat XAR film (Kodak) at k70 mC. Filters were also probed with a mouse MT-I probe [22] and a glyceraldehyde-3-phosphate dehydrogenase cDNA clone [23].…”

Section: Rna Isolation and Northern Blotsmentioning

confidence: 99%

Decreased carbonic anhydrase III levels in the liver of the mouse mutant ‘toxic milk’ (tx) due to copper accumulation

Grimes

Paynter

Walker

et al. 1997

Biochemical Journal

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The mouse mutant ‘toxic milk‘ (tx) is characterized by marked hepatic accumulation of copper, similar to that found in patients with the genetic disorder of copper transport, Wilson disease. In addition, lactating tx females produce copper-deficient milk. To characterize further the biochemical basis of this defect, Western blots of tissue extracts from normal and tx mice were probed with various heavy-metal radioisotopes (63Ni, 65Zn and 64Cu). A 30 kDa Ni/Zn-binding polypeptide was found to be markedly decreased in the livers of the tx mice. This protein was isolated from normal adult mice using a procedure based on Ni-chelation chromatography. The amino acid sequences of two CNBr peptides were identical with portions of the mouse skeletal muscle carbonic anhydrase III (CAIII) sequence. Two other peptides sequenced had closely related sequences to that of CAIII, but with two differences in 45 amino acids. These two peptides may be derived from a novel CAIII isoform, which we term CAIIIB to distinguish it from the published form, CAIIIA. We isolated a cDNA clone corresponding to CAIIIA and used this to show that CAIIIA mRNA was also decreased in the mutant liver, but not in muscle. Copper loading of normal mice also decreased hepatic CAIIIA mRNA, suggesting that the decrease in CAIII mRNA in the tx mouse liver is a secondary consequence of the high copper levels in the liver.

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Post-transcriptional regulation of glyceraldehyde-3-phosphate-dehydrogenase gene expression in rat tissues

Cited by 453 publications

References 32 publications

Rat lactate dehydrogenase A and B subunit concentrations are not regulated by mRNA abundance in liver and heart

Rat lactate dehydrogenase A and B subunit concentrations are not regulated by mRNA abundance in liver and heart

Real-time RT-PCR normalisation; strategies and considerations

Decreased carbonic anhydrase III levels in the liver of the mouse mutant ‘toxic milk’ (tx) due to copper accumulation

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