Post-translational Glycoprotein Modifications Regulate Colon Cancer Stem Cells and Colon Adenoma Progression in Apcmin/+ Mice through Altered Wnt Receptor Signaling

Guo, Hua-Bei; Nagy, Tamás; Pierce, Michael

doi:10.1074/jbc.m114.602680

Cited by 57 publications

(51 citation statements)

References 70 publications

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“…Breast cancer progression and metastasis induced by a viral oncogene in transgenic mice is markedly suppressed in Mgat5-deficient background 73 . Moreover, GnT-V-mediated glycosylation regulates the colon cancer stem cell compartment and tumour progression through WNT signalling 74 .…”

Section: Sialylationmentioning

confidence: 99%

Glycosylation in cancer: mechanisms and clinical implications

2015

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Despite recent progress in understanding the cancer genome, there is still a relative delay in understanding the full aspects of the glycome and glycoproteome of cancer. Glycobiology has been instrumental in relevant discoveries in various biological and medical fields, and has contributed to the deciphering of several human diseases. Glycans are involved in fundamental molecular and cell biology processes occurring in cancer, such as cell signalling and communication, tumour cell dissociation and invasion, cell-matrix interactions, tumour angiogenesis, immune modulation and metastasis formation. The roles of glycans in cancer have been highlighted by the fact that alterations in glycosylation regulate the development and progression of cancer, serving as important biomarkers and providing a set of specific targets for therapeutic intervention. This Review discusses the role of glycans in fundamental mechanisms controlling cancer development and progression, and their applications in oncology.

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Section: Sialylationmentioning

confidence: 99%

Glycosylation in cancer: mechanisms and clinical implications

2015

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“…1 Qualitative and/or quantitative deviations from “normal state” are attributes of numerous human diseases. 2 In particular, aberrations in protein N-glycosylation patterns 3–5 are observed due to different cancer conditions and their clinical stages. 6–7 These changes have been emphasized by several research groups which have demonstrated significant changes to the healthy serum glycomic profiles for a number of cancers.…”

Section: Introductionmentioning

confidence: 99%

Complementary Glycomic Analyses of Sera Derived from Colorectal Cancer Patients by MALDI-TOF-MS and Microchip Electrophoresis

et al. 2016

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Colorectal cancer is the fourth most prevalent cancer in the United States, yet there are no reliable non-invasive early screening methods available. Serum-based glycomic profiling has the necessary sensitivity and specificity to distinguish disease states and provide diagnostic potential for this deadly form of cancer. We applied microchip electrophoresis and MALDI-TOF-MS-based glycomic procedures to 20 control serum samples and 42 samples provided by patients diagnosed with colorectal cancer. Within the identified glycans, the position of fucose units was located to quantitate possible changes of fucosyl isomeric species associated with the pathological condition. MALDI-MS data revealed several fucosylated tri- and tetra-antennary glycans which were significantly elevated in their abundance levels in the cancer samples and distinguished the control samples from the colorectal cancer cohort in the comprehensive profiles. When compared to other cancers studied previously, some unique changes appear to be associated with colorectal cancer, being primarily associated with fucosyl isomers. Through MS and microchip electrophoresis-based glycomic methods, several potential biomarkers were identified to aid in the diagnosis and differentiation of colorectal cancer. With its unique capability to resolve isomers, microchip electrophoresis can yield complementary analytical information to MS-based profiling.

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“…Recently, we have confirmed that ␤1,4GalTV could regulate the self-renewal of glioma-initiating cells (19). N-acetylglucosaminyltransferase V promotes the self-renewal and tumorigenicity of colon cancer stem cells (20). The N-glycan inhibitor tunicamycin inhibits the self-renewal ability and tumorigenesis of glioma stem-like cells (21).…”

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confidence: 76%

β1,4-Galactosyltransferase V activates Notch1 signaling in glioma stem-like cells and promotes their transdifferentiation into endothelial cells

Cui

Chen

Liu

et al. 2018

Journal of Biological Chemistry

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Malignant glioblastoma multiforme is one of the most aggressive human cancers, with very low survival rates. Recent studies have reported that glioma stem-like cells transdifferentiate into endothelial cells, indicating a new mechanism for tumor angiogenesis and potentially providing new therapeutic options for glioblastoma treatment. Glioma malignancy is strongly associated with altered expression of -linked oligosaccharide structures on the cell surface. We have previously reported that β1,4-galactosyltransferase V (β1,4GalTV), which galactosylates the GlcNAcβ1-6Man arm of the branched-glycans, is highly expressed in glioma and promotes glioma cell growth and However, the mechanism by which β1,4GalTV stimulates glioma growth is unknown. Here we demonstrate that short hairpin RNA-mediated β1,4GalTV knockdown inhibits the tumorigenesis of glioma stem-like cells and reduces their transdifferentiation into endothelial cells. We also found that β1,4GalTV overexpression increased glioma stem-like cell transdifferentiation into endothelial cells and that this effect required β1,4GalTV galactosylation activity. Moreover, β1,4GalTV promoted β1,4-galactosylation of Notch1 and increased Notch1 protein levels. Of note, ectopic expression of activated Notch1 rescued the inhibitory effect of β1,4GalTV depletion on glioma stem-like cell transdifferentiation. In summary, our findings indicate that β1,4GalTV stimulates transdifferentiation of glioma stem-like cells into endothelial cells by activating Notch1 signaling. These detailed insights shed important light on the mechanisms regulating glioma angiogenesis.

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Post-translational Glycoprotein Modifications Regulate Colon Cancer Stem Cells and Colon Adenoma Progression in Apcmin/+ Mice through Altered Wnt Receptor Signaling

Cited by 57 publications

References 70 publications

Glycosylation in cancer: mechanisms and clinical implications

Glycosylation in cancer: mechanisms and clinical implications

Complementary Glycomic Analyses of Sera Derived from Colorectal Cancer Patients by MALDI-TOF-MS and Microchip Electrophoresis

β1,4-Galactosyltransferase V activates Notch1 signaling in glioma stem-like cells and promotes their transdifferentiation into endothelial cells

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